A5019919335- Phytochemistry and Bioactive Compounds
- Phytochemical compounds biological activities
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Natural product bioactivities and synthesis
- Carbohydrate Chemistry and Synthesis
- Protein Hydrolysis and Bioactive Peptides
- Transgenic Plants and Applications
- Biochemical and Structural Characterization
- Toxin Mechanisms and Immunotoxins
- Phytochemistry Medicinal Plant Applications
- Legume Nitrogen Fixing Symbiosis
- Insect Pest Control Strategies
- Marine Sponges and Natural Products
- Proteins in Food Systems
- Cynara cardunculus studies
- Pineapple and bromelain studies
- Polysaccharides and Plant Cell Walls
- Vitamin K Research Studies
- Agricultural pest management studies
- Phytochemistry and Bioactivity Studies
- Insect Resistance and Genetics
- African Botany and Ecology Studies
- Essential Oils and Antimicrobial Activity
- Plant-Derived Bioactive Compounds
Universidade Federal do Ceará
2016-2025
Universidade de Fortaleza
2021
Universidad Nacional de La Plata
2016
National University of San Luis
2016
GTx (United States)
2013
Universidade Federal do Piauí
2011
Albert Einstein College of Medicine
2000
The emergence of antibiotic-resistant microbes has stimulated research worldwide seeking new biologically active molecules. In this respect, synthetic antimicrobial peptides (SAMPs) have been suggested to overcome problem. Although there are some online servers that provide putative SAMPs from protein sequences, the choice best peptide sequences for further analysis is still difficult. Therefore, goal paper not launch a tool but friendly workflow characterize and predict potential by...
Abstract Living cells are constantly exchanging momentum with their surroundings. So far, there is no consensus regarding how respond to such external stimuli, although it reveals much about internal structures, motility as well the emergence of disorders. Here, we report that twelve cell lines, ranging from healthy fibroblasts cancer cells, hold a ubiquitous double power-law viscoelastic relaxation compatible fractional Kelvin-Voigt model. Atomic Force Microscopy measurements in time domain...
The antiproliferative activity of lectins Canavalia ensiformis (ConA) and brasiliensis (ConBr) were studied using human leukemia MOLT-4 HL-60 cell lines. It was revealed that both ConA ConBr markedly cytotoxic to cells MTT NAC assays. IC(50) values approximately 3 20 μg/mL for ConBr, respectively, cells. However, in normal peripheral blood lymphocytes, the not cytotoxic, even when tested at concentrations as high 200 μg/ml. Using comet assay, produced a rate DNA damage exceeding 80%...
This study aimed at investigating the structural properties and mechanisms of antifungal action CpOsm, a purified osmotin from Calotropis procera latex. Fluorescence CD assays revealed that CpOsm structure is highly stable, regardless pH levels. Accordingly, inhibited spore germination Fusarium solani in all ranges tested. The content secondary was estimated as follows: α-helix (20%), β-sheet (33%), turned (19%) unordered (28%), RMSD 1%. stable up to 75 °C, thermal denaturation (Tm)...
The latex of Calotropis procera is a rich source proteolytic activity. This known to contain two distinct cysteine peptidases: procerain and B. In this study, new peptidases were purified from C. latex. enzymes by sequential ion-exchange chromatography steps (CM-Sepharose plus Resource S(®)) at pH 5.0 6.0. had molecular mass spectra corresponding CpCP-1=26,213, CpCP-2=26,133 CpCP-3=25,086 Da. These exhibited discrete differences in terms enzymatic activity broad range temperature conditions...
Abstract Photobiomodulation (PBM) is a promising non‐invasive therapy for tissue repair, but its underlying cellular mechanisms are not fully understood. In this study, the biomechanical and proteomic responses of three cell types – keratinocytes (HACAT), fibroblasts (L929), osteoblasts (OFCOLII) exposed to red light (633 nm) investigated using atomic force microscopy (AFM) mass spectrometry‐based analysis. Red absorption resulted in cell‐type‐specific changes viscoelastic properties, with...
Calotropis procera R. Br. (Asclepiadaceae) is a well-known medicinal plant with leaves, roots, and bark being exploited by popular medicine to fight many human animal diseases. This work deals the fractionation of crude latex produced green parts aims evaluate its toxic effects upon egg hatching larval development Aedes aegypti. The whole was shown cause 100% mortality 3rd instars within 5 min. It fractionated into water-soluble dialyzable (DF) non-dialyzable (NDF) rubber-free materials....
Extracts rich in cysteine proteases obtained from fruits of Pseudananas macrodontes (Pm), Bromelia balansae (Bb), and B. hieronymi (Bh) have previously shown an anti-inflammatory effect on animal models. Given the close relationship between hemostasis inflammation, it is attractive to investigate therapeutic agents capable modulating both systems. The aim this work was study Pm, Bb, Bh fibrin(ogen) blood coagulation compared with stem bromelain (Bro). Action fibrinogen electrophoretically...
Latex from Calotropis procera is widely used in folk medicine as a rich source of biologically active compounds capable promoting diverse benefits such control dermal fungal infections, antimicrobial activities and pain relief among other useful properties. The aim this work was to characterize the anti-inflammatory effect non-dialysable protein fraction recovered rubber-free latex using three different experimental models when administrated intravenously. In vivo neutrophil migration...
The latex of Calotropis procera has been used in traditional medicine to treat different inflammatory diseases. anti-inflammatory activity proteins (LP) well documented using models. In this work the protein fraction was evaluated a true process by inducing lethal experimental infection murine model caused Salmonella enterica Subsp. serovar Typhimurium.Experimental Swiss mice were given 0.2 ml LP (30 or 60 mg/kg) intraperitoneal route 24 h before after challenge (0.2 ml) containing 10(6)...