A5025589081- Sperm and Testicular Function
- Glycosylation and Glycoproteins Research
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Phytochemistry and Bioactive Compounds
- Reproductive Biology and Fertility
- Phytochemical compounds biological activities
- Biochemical and Structural Characterization
- Polysaccharides Composition and Applications
- Natural product bioactivities and synthesis
- Agricultural pest management studies
- Enzyme Production and Characterization
- Bioactive natural compounds
- Carbohydrate Chemistry and Synthesis
- Studies on Chitinases and Chitosanases
- Clusterin in disease pathology
- Toxin Mechanisms and Immunotoxins
- Plant Stress Responses and Tolerance
- Biochemical Analysis and Sensing Techniques
- Phytochemistry Medicinal Plant Applications
- Moringa oleifera research and applications
- Proteins in Food Systems
- Enzyme Structure and Function
- Microbial Metabolites in Food Biotechnology
- Neuroscience and Neuropharmacology Research
Universidade de Fortaleza
2016-2025
Universidade Estadual do Ceará
2021-2024
Universidade Federal do Ceará
2001-2024
Unifor
2016
Universidade de São Paulo
2001-2002
Universidade Federal de São Carlos
2001
The present study was conducted to identify the major seminal plasma protein profile of boars and its associations with semen criteria. Semen samples were collected from 12 adult subjected evaluation sperm parameters (motility, morphology, vitality, percent cells intact acrosome). Seminal obtained by centrifugation, analyzed two-dimensional SDS-PAGE, proteins identified mass spectrometry (electrospray ionization quadrupole time-of-flight). We tested regression models using spot intensities...
Mo-CBP3 is a chitin-binding protein from M. oleifera seeds that inhibits the germination and mycelial growth of phytopathogenic fungi. This highly thermostable resistant to pH changes, therefore may be useful in development new antifungal drugs. However, relationship MoCBP3 with known families carbohydrate-binding domains has not been established. In present study, full-length cDNAs encoding 4 isoforms (Mo-CBP3-1, Mo-CBP3-2, Mo-CBP3-3 Mo-CBP3-4) were cloned developing seeds. The polypeptides...
Neem fruit (Azadirachta indica A. Juss.) are popularly used to treat infections, diarrhea, fever, bronchitis, skin diseases, infected burns and hypertension. Although the antinociceptive anti-inflammatory potential of has already been investigated in experimental models pain inflammation mice, current research is first report evaluation capacity ethanolic extract (EtFrNeem) acute attenuation using adult zebrafish (Danio rerio) as an alternative model use rodents. EtFrNeem was submitted...
Abstract Living cells are constantly exchanging momentum with their surroundings. So far, there is no consensus regarding how respond to such external stimuli, although it reveals much about internal structures, motility as well the emergence of disorders. Here, we report that twelve cell lines, ranging from healthy fibroblasts cancer cells, hold a ubiquitous double power-law viscoelastic relaxation compatible fractional Kelvin-Voigt model. Atomic Force Microscopy measurements in time domain...
The latex of Calotropis procera is a rich source proteolytic activity. This known to contain two distinct cysteine peptidases: procerain and B. In this study, new peptidases were purified from C. latex. enzymes by sequential ion-exchange chromatography steps (CM-Sepharose plus Resource S(®)) at pH 5.0 6.0. had molecular mass spectra corresponding CpCP-1=26,213, CpCP-2=26,133 CpCP-3=25,086 Da. These exhibited discrete differences in terms enzymatic activity broad range temperature conditions...
Acute lymphoblastic leukemia is the most common malignant cancer in childhood. The signs and symptoms of childhood are difficult to recognize, as it not first diagnosis be considered for nonspecific complaints, leading potential uncertainty diagnosis. aim this study was perform proteomic analysis serum from pediatric patients with B-cell acute (B-ALL) identify candidate biomarker proteins, use early evaluation treatment. Serum samples were obtained ten at time (B-ALL group) after induction...
The present study evaluated the effects of heat stress on ram seminal plasma proteome. Six Morada Nova rams were scrotal insulated for 8 days. Scrotal circumference, sperm parameters, and fluid proteins before (Day 0) twice during insulation (Days 4 8), weekly until semen parameters returned to preinsulation values (normal). Seminal analyzed by two-dimensional SDS-PAGE mass spectrometry. circumference decreased from 30 ± 0.4 cm Day 0 22.6 0.6 36 (P < 0.05) became equivalent 71. Motile nearly...
Jatropha curcas seed cake is a low-value by-product resulting from biodiesel production. The highly toxic, but it has great potential for biotechnology applications as repository of biomolecules that could be important in agriculture, medicine and industry. To explore this potential, novel trypsin inhibitor called JcTI-I was purified by fractionation the crude extract with trichloroacetic acid (2.5%, v/v) followed affinity chromatography (Trypsin-Sepharose 4B) molecular exclusion (Sephacryl...
The objective of the present study was to describe relationship seminal plasma and total sperm cell proteins with semen freezability parameters Guzerat bulls. Thirteen bulls were subjected breeding soundness evaluation. Semen samples collected, cryopreserved, then post-thawing kinetics assessed, where high (n = 7) low 6) groups defined. Seminal from 2 separated by 2-dimensional SDS-PAGE, spots identified mass spectrometry. post-cryopreservation as follows in groups, respectively: mean...
Candida species are opportunistic pathogens that infect immunocompromised and/or immunosuppressed patients, particularly in hospital facilities, besides representing a significant threat to health increase the risk of mortality. Apart from echinocandins and triazoles, which well tolerated, most antifungal drugs used for candidiasis treatment can cause side effects lead development resistant strains. A promising alternative conventional treatments is use plant proteins. M. oleifera Lam. with...
Compounds exhibiting potent antiviral activity against the novel coronavirus remain insufficiently understood, posing significant challenges in development of effective therapeutic interventions. Among various classes bioactive molecules, terpenes represent a promising group natural compounds with diverse and extensive properties. This study aims to explore, through advanced silico approaches, potential four specific terpenes-α-bisabolol, citral, cis-jasmone, eucalyptol-against spike...
During the COVID-19 pandemic, remote learning demanded rapid adaptation and continuous innovation in educational environment. This paper examines how scientific-technological advances digital tools have been incorporated into teaching through active methodology of "maker culture". A literature review was conducted to evaluate this approach, highlighting Information Communication Technologies (ICT) their impact on basic education. While implementing these technologies offers innovative...
The objective was to describe the profile of membrane proteins from sperm tropically adapted Morada Nova rams (N = 5). Samples protein-enriched fractions ejaculated (containing 400 μg protein) were separated by two-dimensional electrophoresis and respective maps analyzed using PDQuest software (version 7.3.0; Bio-Rad). Proteins identified tandem mass spectrometry. Also, incubated with antibodies against binder protein (BSP) 1 bodhesin 2 (Bdh-2), components vesicular gland secretion. For...
A trypsin inhibitor from Cassia leiandra seeds, named ClTI, was purified, characterized, and its insecticidal activity against Aedes aegypti evaluated. ClTI purified by DEAE-Cellulose trypsin-Sepharose 4B chromatography, with a 15.5-fold purification 2.4% yield. is composed of 19,484 Da polypeptide chain as revealed mass spectrometry, it not glycoprotein, amino acid sequence similar to other Kunitz-type inhibitors, comprises 35% β-sheets, 14% β-turns, 50% disordered secondary structures. an...