A5084357560- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Renal and related cancers
- Sperm and Testicular Function
- Genetic and phenotypic traits in livestock
- Pluripotent Stem Cells Research
- Birth, Development, and Health
- Ovarian function and disorders
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Pregnancy and preeclampsia studies
- Infant Nutrition and Health
- Seed Germination and Physiology
- Ruminant Nutrition and Digestive Physiology
- Bioenergy crop production and management
- Child Nutrition and Water Access
- RNA Interference and Gene Delivery
- Prenatal Screening and Diagnostics
- Reproductive Health and Technologies
- Reproductive System and Pregnancy
- Lipid metabolism and biosynthesis
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic Syndromes and Imprinting
Universidade Federal do Rio Grande do Sul
2016-2025
Universidade do Estado de Santa Catarina
2007-2024
Universidade Federal de São Paulo
2024
Universidade Federal do Rio Grande
2020-2021
Division of Undergraduate Education
2021
Universidade de Fortaleza
2010-2019
University of California System
2018
University of California, Davis
2001-2012
Universidad Nacional de Colombia
2009
University of Guelph
2004
The effects of the embryo production system on growth and transcription rate day 7 16 bovine embryos were investigated. In vivo- (controls) in vitro-produced (IVP) transferred to female recipients development, allowed develop a synchronous uterine environment 16. Embryonic transcripts for insulin-like factors-1 -2 (IGF-1 -2), their receptors (IGF-1r -2r), facilitative glucose transporters-1 -3 (Glut-1 -3), interferon-tau (IFN-tau) determined by real-time quantitative PCR (TaqMan); gender...
Changes in placental development have been associated with foetal abnormalities after vitro embryo manipulations. This study was designed to investigate bovine conceptus and substrate levels plasma fluids vivo- vitro-produced (IVP) concepti neonates. In vivo-produced IVP embryos were derived by established production procedures. Pregnant animals from both groups slaughtered on days 90 or 180 of gestation, allowed go term. Conceptus neonatal physical traits recorded; foetal, maternal blood,...
Animal cloning has been associated with developmental abnormalities, the level of heteroplasmy caused by procedure being one its potential limiting factors. The aim this study was to determine effect fusion hemicytoplasts or aggregation hemiembryos, varying final cytoplasmic volume, on development and cell density embryos produced hand-made (HMC), parthenogenesis in vitro fertilization (IVF). One two enucleated were paired fused skin somatic cell. Activated clone zona-free parthenote...
Assisted reproduction techniques have improved agricultural breeding in the bovine. However, important development steps may differ from situation vivo and there is a high mortality rate during first trimester of gestation. To better understand these events, we investigated embryos fetal membranes following fixed-time AI (FTAI), IVF nuclear transfer (NT). The onset yolk-sac was not normal cloned embryos. Later differed conditions all three groups; yellowish juxtaposed with amniotic membrane....
Malnutrition remains a leading contributor to the morbidity and mortality of children under age five worldwide. However, underlying mechanisms are not well understood necessitating an appropriate animal model answer fundamental questions conduct translational research into optimal interventions. One potential intervention is milk from livestock that more closely mimics human by increased levels bioactive components can promote healthy intestinal epithelium. We tested ability cow transgenic...
This study aimed to establish a culture system that improves the in vitro development of caprine preantral follicles. In first experiment, follicles were encapsulated as single unit per bead and cultured singly or groups with five same alginate (ALG) for 18 days. subsequent "five bead" design was chosen ALG, fibrin-alginate (FA) hyaluronate (HA) third we chose FA 30 The set-up ALG increased antrum formation follicle diameter compared other designs (p < .05). Moreover, under this condition,...
Background Diarrheal diseases are an important cause of morbidity and mortality among children in developing countries. We aimed to study the etiology severity diarrhea living low-income semiarid region Brazil. Methodology This is a cross-sectional, age-matched case-control aged 2–36 months from six cities Brazil's region. Clinical, epidemiological, anthropometric data were matched with fecal samples collected for identification enteropathogens. Results enrolled 1,200 children, 596 cases 604...
ABSTRACT This study aimed to determine the relationship between echogenicity and heterogeneity of ovarian structures cyclicity status, estrus expression pregnancy per artificial insemination (P/AI) in multiparous beef cows during timed‐artificial (TAI). An ultrasound examination was carried out 406 crossbred suckled at progesterone (P4) insert removal on D8 a TAI protocol diagnosis 30 days after TAI. Follicular (antrum, wall perifollicular stroma) luteal morphometry, echotexture parameters...
ABSTRACT The study aimed to evaluate the effects of GnRH given 34 h after progesterone (P4) intravaginal device (IVD) removal in a timed artificial insemination (TAI) protocol. In Experiment 1, 17 Hereford and Braford heifers (control, n = 7; GnRH34; 10) received 2 mg estradiol benzoate (EB) 1 g P4 IVD on Day 0 (D0). On D8, IVDs were removed 150 μg d‐cloprostenol (PGF) cypionate (EC) administered. GnRH34 group, animals 25 lecirelin (D9). TAI was performed both groups D10 (48 removal)....
The period immediately after birth is a vital time for all newborn calves as the cardiovascular, respiratory, and other organ systems adapt to life ex utero. Reported neonatal mortality rates suggest this be especially critical in cloned calves; yet prospective, controlled studies on physiological status of these are lacking. objectives study were compare (birth 48 h age) physical clinical characteristics placental morphology embryo transfer control delivered by cesarean section induced...
The effects of insulin-like growth factor-I (IGF-I) and its interaction with gonadotropins, estradiol, fetal calf serum (FCS) on in vitro maturation (IVM) equine oocytes were investigated this study. We also examined the role IGF-I presence or absence FCS parthenogenic cleavage after oocyte activation calcium ionophore combined 6-dimethylaminopurine (6-DMAP), using rate as a measure cytoplasmic maturation. Only cumulus-oocyte complexes compact cumulus homogenous ooplasm (n = 817) used. In...
Potential applications of somatic cell nuclear transfer to agriculture and medicine are currently constrained by low efficiency high rates embryonic, fetal, neonatal loss. Nuclear in cattle was compared between three donor-cell treatments from a single animal, four sequential stages differentiation lineage genotype, the same type two donors. Cumulus granulosa donor cells resulted greater proportion viable day-7 embryos than ear-skin cells; pregnancy rate losses were not different among...
Bovine conceptus development and its association with placental proteins present in maternal, foetal neonatal plasma (amniotic allantoic) fluids were investigated vivo - vitro -produced (IVP) concepti newborn calves. Females superovulated to obtain control embryos, whereas IVP embryos derived from established procedures. Pregnant animals slaughtered on days 90 or 180 of gestation allowed develop term for the assessment physical traits. Foetal, maternal blood collected determination bovine...
Although a majority of clones are born normal and apparently healthy, mortality rates nearly 30% described in many reports. Such losses major limitation cloning technology represent substantial economic investment as well justifiable animal health welfare concerns. Prospective, controlled studies needed to understand fully the causes neonatal develop preventive therapeutic strategies minimize losses. We report here findings on hematologic biochemical profiles cloned control calves immediate...
Our objective was to investigate the lipid content of uterus, blood plasma, and milk at early, mid, late diestrus. Lactating cows (n = 30) had estrous cycle ovulation synchronized by administration exogenous hormones. Cows were blocked parity assigned randomly receive transcervical uterine flushing biopsy on d 5 (early diestrus), 10 (mid diestrus) or 15 (late cycle. Flushing endometrial performed in horn ipsilateral corpus luteum. The recovered used for analyses composition liquid...
We investigated the effect of KL and IGF-I, alone or in combination, on vitro survival, growth antrum formation sheep preantral follicles, subsequent oocyte developmental competence. Preantral follicles ∼200 μm were isolated cultured for 18 days basic medium (control) added not by KL, IGF-I both. Every six follicular was evaluated. At end culture period, oocytes underwent maturation (IVM) their viability chromatin configuration assessed. To evaluate quality its competence, parthenogenetic...
Cloning by somatic cell nuclear transfer (SCNT) is characterized low efficiency and the occurrence of developmental abnormalities, which are rather poorly studied phenomena in goats. This study aimed at comparing overall SCNT goats using vitro-matured (IVM) or vivo-matured oocytes fibroblast donor cells (mock transfected, transgenic, wild type), also characterizing symptoms Abnormal Offspring Syndrome (AOS) development, results with pregnancies produced artificial insemination (AI)...