A5074125239- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Trypanosoma species research and implications
- CRISPR and Genetic Engineering
- Research on Leishmaniasis Studies
- Lysosomal Storage Disorders Research
- Reproductive Physiology in Livestock
- Renal and related cancers
- Viral Infectious Diseases and Gene Expression in Insects
- Pain Mechanisms and Treatments
- Trace Elements in Health
- Virus-based gene therapy research
- Biochemical and Molecular Research
- Ion Channels and Receptors
- Selenium in Biological Systems
- Nitrogen and Sulfur Effects on Brassica
- Parasitic Infections and Diagnostics
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- Pesticide Exposure and Toxicity
- Monoclonal and Polyclonal Antibodies Research
- Birth, Development, and Health
- Effects of Environmental Stressors on Livestock
- Neurobiology and Insect Physiology Research
- DNA Repair Mechanisms
Universidade de Fortaleza
2014-2024
Universidade do Estado de Santa Catarina
2008-2024
Universidade Estadual do Ceará
2023
Fundação Edson Queiroz
2023
This study aimed to evaluate the antinociceptive effect of oleanolic acid using adult zebrafish models orofacial pain. Acute nociception was induced by formalin, capsaicin, cinnamaldehyde, menthol, acidified saline or glutamate (cutaneous modes) and hypertonic (corneal model). In another set experiments, animals were pre-treated with naloxone, L-NAME, methylene blue, ketamine, camphor, HC-030031, mefenamic acid, ruthenium red amiloride investigate mechanism antinociception. The involvement...
Cloning by somatic cell nuclear transfer (SCNT) is characterized low efficiency and the occurrence of developmental abnormalities, which are rather poorly studied phenomena in goats. This study aimed at comparing overall SCNT goats using vitro-matured (IVM) or vivo-matured oocytes fibroblast donor cells (mock transfected, transgenic, wild type), also characterizing symptoms Abnormal Offspring Syndrome (AOS) development, results with pregnancies produced artificial insemination (AI)...
Castration is used to improve the management of water buffalo beef males raised under extensive conditions. However, as are considered robust animals, their welfare often neglected, which, among other implications, may compromise productivity. The aim this study was determine effects different castration methods on stress level and weight gain males. Two experiments were performed with three treatments each. In experiment 1, serum cortisol concentrations indicators for non-castrated (control...
This study evaluates the pharmacological potential of cis-jasmone (CJ) in adult zebrafish (Danio rerio; aZF). Initially, aZF (n = 6/group) were pretreated (20 µL; p. o.) with CJ (0.1 or 0.3 1.0 mg/mL) vehicle (0.5% Tween 80). The animals submitted to acute toxicity and locomotion tests, pentylenetetrazole-induced seizure, carrageenan-induced abdominal edema, cinnamaldehyde-, capsaicin-, menthol-, glutamate-, acid saline-induced orofacial nociception. possible mechanisms anticonvulsant,...
The imprinted H19 long non-coding RNA, a knowing oncofetal gene, presents controversial role during the carcinogenesis process since its tumor suppressor or oncogenic activity is not completely elucidated. Since lncRNA involved in many biological pathways related to tumorigenesis, we sought develop non-cancer lineage with CRISPR-Cas9-mediated knockdown (H19-) and observe changes cellular context. To edit promoter region of H19, two RNA guides were designed, murine C2C12 myoblast cells...
O objetivo deste estudo foi investigar a patogenicidade do isolado de Trypanosoma evansi (LPV-2005), em ratos (Rattus norvergicus), sob influência da imunidade passiva, diferentes concentrações e meios conservação. Para tanto, foram utilizados 36 Rattus norvergicus, fêmeas, separados seis grupos homogêneos. Os roedores dos A B infectados com 10(5) T. evansi, os animais C D 10(6) tripomastigotas/animal. E F como grupo controle negativo, isto é, inoculados sangue in natura criopreservado sem o...
Embryo sexing is a powerful tool for livestock producers because it allows them to manage their breeding stocks more effectively.However, the cost of supplies and reagents, need trained professionals biopsy embryos by micromanipulation restrict worldwide use technology limited number specialized groups.The aim this study was couple fast inexpensive DNA extraction protocol with practical approach create simple, quick, effective, dependable embryo procedure.From total 1847 sheep cattle whole...
L-Asparaginase (ASNase) is an enzyme used in the treatment of acute lymphoblastic leukemia (ALL). As therapeutic ASNases has bacterial origin, severe side effects are associated with its use, among them hypersensitivity and inactivation enzyme. In this context, objective work was to produce a recombinant ASNase origin human cells order determine presence consequences potential post-translational modifications on enzyme.Recombinant expressed molecular weight 60 kDa, larger than Escherichia...
Em resposta a uma denúncia da existência de triatomíneos em cabana pesca as margens do Rio Caveiras no município São José Cerrito-SC, foi feita investigação na e outros ecótopos vizinhos ao achado inicial. Foram encontrados 15 exemplares Panstrongylus megistus sendo que nenhum deles encontrava-se infectado. O local ecótopo é visitado ocasionalmente por capivaras, gambás, tatus ratos.
INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, decrease specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high-coverage complete SARS-CoV-2 genome sequences from 13 Brazilian states were aligned with 15 WHO recommended assays. RESULTS: Only 3 completely all sequences. Ten had mismatches up and...
Several chemical and immunohistochemical techniques can be used for the detection of acetylcholinesterase (AChE) activity. In this experiment we aimed to detect AChE activity in Trypanosoma evansi. For this, parasites were isolated from blood experimentally infected rats using a DEA-cellulose column. Enzymatic was determined trypomastigote forms at 0, 0.2, 0.4, 0.8 1.2 mg/mL protein concentrations by standard biochemical protocol. At all tested, study showed that T. evansi expresses enzyme...