A5021587514- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Renal and related cancers
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Prenatal Screening and Diagnostics
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Genetic Syndromes and Imprinting
- Adipose Tissue and Metabolism
- Pregnancy and preeclampsia studies
- Genetic and phenotypic traits in livestock
- Birth, Development, and Health
- Diet and metabolism studies
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Bioenergy crop production and management
- Virus-based gene therapy research
Universidade de São Paulo
2016-2025
Universidade Brasil
2023-2025
Division of Undergraduate Education
2021-2023
Institute of Developmental Physiology
2023
Universidade Cidade de São Paulo
2020
Hospital Universitário da Universidade de São Paulo
2015
Associação Brasileira de Oncologia Veterinária
2009-2014
Seevic College
2011
Universidade Estadual Paulista (Unesp)
2007-2010
Pregnancy success results from the interaction of multiple factors, among them are folliculogenesis and early embryonic development. Failure during these different processes can lead to difficulties in conception. Alternatives overcome problems based on assisted reproductive techniques. Extracellular vesicles cell-secreted present body fluids contain bioactive materials, such as messenger RNA, microRNAs (miRNAs), proteins. Thus, our hypothesis is that extracellular follicular fluid 3–6 mm...
Methods used for lipid analysis in embryos and oocytes usually involve selective extraction from a pool of many samples followed by chemical manipulation, separation characterization individual components chromatographic techniques. Herein we report direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) single intact or various species. Biological were simply moisturized with the matrix solution characteristic (represented phosphatidylcholines, sphingomyelins...
The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests dynamic interplay among follicular cells oocytes, since they are constantly exchanging "messages". We dissected bovine follicles recovered (FCs-granulosa cumulus cells) cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following rupture, COCs were individually selected for vitro cultures track based...
Abstract Oocytes that undergo in vitro maturation (IVM) are metabolically abnormal and accumulate excess lipid content. However, the mechanism of accumulation role cumulus cells this process unclear. Recently, it was shown fatty acid binding proteins (FABPs) performed intra- extracellular transport. We postulated FABP3 might be responsible for transport from to oocytes via transzonal projections (TZPs) during IVM. Transcript protein levels were analyzed both vivo - -matured...
Abstract Extracellular vesicles (EVs) are nanoparticles secreted by ovarian follicle cells. an important form of intercellular communication, since they carry bioactive contents, such as microRNAs (miRNAs), mRNAs, and proteins. MicroRNAs small noncoding RNA capable modulating mRNA translation. Thus, EVs can play a role in oocyte development. However, it is not clear if EV contents vary with the estrous cycle stage. The aim this study was to investigate bovine miRNA content obtained from...
Abstract Background It is well described that circulating progesterone (P4) plays a key role in several reproductive events such as oocyte maturation. However, during diestrus, when P4 at the highest concentrations, little known about its local impact on follicular cells intrafollicular concentration due to corpus luteum (CL) presence within same ovary. Based that, our hypothesis CL ovary diestrus alters competence acquisition, gene expression, and small extracellular vesicles (sEVs) miRNAs...
Abstract Semen fertilizing potential is dependent upon the morphological, functional and molecular attributes of sperm. Sperm microRNAs (miRNAs) were recently shown to hold promise regarding their association with different fertility phenotypes. However, role in regulation remains be determined. We postulated that sperm miRNAs might regulate early embryonic development. From this perspective, quality 380 investigated frozen–thawed semen from high (HF; 54.3 ± 1.0% pregnancy rate) low (LF;...
Although cloning of mammals has been achieved successfully, the percentage live offspring is very low because reduced fetal size and fewer implantation sites. Recent studies have attributed such pathological conditions to abnormal reprogramming donor cell used for cloning. The inability oocyte fully restore differentiated status a somatic its pluripotent undifferentiated state normally evidenced by aberrant DNA methylation patterns established throughout genome during development blastocyst....
Although somatic cell nuclear transfer (SCNT) is a promising tool, its potential use hampered by the high mortality rates during development to term of cloned offspring. Abnormal epigenetic reprogramming donor nuclei after SCNT thought be main cause this low efficiency. We hypothesized that chromatin-modifying agents (CMAs) targeting chromatin acetylation and DNA methylation could alter configuration turn them more amenable reprogramming. Thus, bovine fibroblasts were treated with...
Cloning of mammals by somatic cell nuclear transfer (SCNT) is still plagued low efficiency. The epigenetic modifications established during cellular differentiation are a major factor determining this efficiency as they act barriers restricting reprogramming nuclei. In regard, most factors that promote chromatin decondensation, including histone deacetylase inhibitors (HDACis), have been found to increase efficiency, making their use common improve SCNT rates. Herein we used valproic acid...
Contents The ovarian follicle components must provide an ideal environment to ensure the success of reproductive processes, and communication between follicular cells is crucial support proper oocyte growth. Recently, it has been demonstrated that presence extracellular vesicles (EVs) carrying microRNAs (miRNAs) in fluid represents important autocrine paracrine mechanism inside follicle. In this study, we tested hypothesis miRNA content EVs isolated from (granulosa cumulus–oocyte complexes)...
The influence of in vitro maturation (IVM) oocytes is still not totally understood. aim this study was to determine the IVM on metabolism and homeostasis bovine cumulus-oocyte complexes. In present study, we demonstrated that leads accumulation neutral lipids associated with differential levels mono-, di- triacylglycerols both cumulus cells oocytes. We observed vitro-matured exhibited decreased glutathione reactive oxygen species a lower ATP/ADP ratio when compared vivo-matured oocytes, no...
Abstract Nuclear reprogramming mediated by somatic cell nuclear transfer (SCNT) has many applications in medicine. However, animal clones show increased rates of abortion and reduced neonatal viability. Herein, we used exosomal-miRNA profiles as a non-invasive biomarker to identify pathological pregnancies. MiRNAs play important roles cellular proliferation differentiation during early mammalian development. Thus, the aim this study was exosomal-miRNAs maternal blood at 21 days gestation...
Abstract We evaluated the effect of antral follicle count (AFC) on ovarian follicular dynamics, pregnancy rates, progesterone concentrations, and transcriptional patterns genes in Nelore cattle ( Bos taurus indicus ) after a timed artificial insemination (TAI) programme. Cows were separated based AFC, those with high AFC showed larger (P < 0.0001) diameter area than very low AFC. Females exhibited 0.01) dominant at TAI (13.6 ± 0.3 vs. 12.2 0.4 mm) tendency = 0.06) to have different serum...
Early embryonic development occurs in the oviduct, where an ideal microenvironment is provided by epithelial cells and oviductal fluid produced these cells. The contains small extracellular vesicles (sEVs), which through their contents, including microRNAs (miRNAs), can ensure proper cell communication between mother embryo. However, little known about modulation of miRNAs within (OECs) sEVs from pregnant cows. In this study, we evaluate profile flushing (OF-sEVs) OECs cows compared to...
Ooplasmic transfer (OT) has been used in basic mouse research for studying the segregation of mtDNA, as well human assisted reproduction improving embryo development cases persistent developmental failure. Using cattle a large-animal model, we demonstrate that moderate amount mitochondria introduced by OT is transmitted to offspring's oocytes; e.g., modifies germ line. The donor mtDNA was detectable 25% and 65% oocytes collected from two females. Its high variation heteroplasmic oocytes,...
Assisted reproduction techniques have improved agricultural breeding in the bovine. However, important development steps may differ from situation vivo and there is a high mortality rate during first trimester of gestation. To better understand these events, we investigated embryos fetal membranes following fixed-time AI (FTAI), IVF nuclear transfer (NT). The onset yolk-sac was not normal cloned embryos. Later differed conditions all three groups; yellowish juxtaposed with amniotic membrane....