A5029367598- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- Pregnancy and preeclampsia studies
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Ovarian function and disorders
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Renal and related cancers
- Genetic and phenotypic traits in livestock
- Vitamin D Research Studies
- Adenosine and Purinergic Signaling
- Mesenchymal stem cell research
- Pain Mechanisms and Treatments
- Vector-Borne Animal Diseases
- Pickering emulsions and particle stabilization
- TGF-β signaling in diseases
- Diet and metabolism studies
- Adipokines, Inflammation, and Metabolic Diseases
Universidade de São Paulo
2018-2025
University of the West
2025
Universidade do Oeste de Santa Catarina
2024-2025
Universidade Brasil
2022-2024
Institute of Developmental Physiology
2023
Division of Undergraduate Education
2023
Universidade Federal de Santa Maria
2012-2019
Abstract Background It is well described that circulating progesterone (P4) plays a key role in several reproductive events such as oocyte maturation. However, during diestrus, when P4 at the highest concentrations, little known about its local impact on follicular cells intrafollicular concentration due to corpus luteum (CL) presence within same ovary. Based that, our hypothesis CL ovary diestrus alters competence acquisition, gene expression, and small extracellular vesicles (sEVs) miRNAs...
Abstract Extracellular vesicles (EVs) are nanoparticles secreted by ovarian follicle cells. an important form of intercellular communication, since they carry bioactive contents, such as microRNAs (miRNAs), mRNAs, and proteins. MicroRNAs small noncoding RNA capable modulating mRNA translation. Thus, EVs can play a role in oocyte development. However, it is not clear if EV contents vary with the estrous cycle stage. The aim this study was to investigate bovine miRNA content obtained from...
Early embryonic development occurs in the oviduct, where an ideal microenvironment is provided by epithelial cells and oviductal fluid produced these cells. The contains small extracellular vesicles (sEVs), which through their contents, including microRNAs (miRNAs), can ensure proper cell communication between mother embryo. However, little known about modulation of miRNAs within (OECs) sEVs from pregnant cows. In this study, we evaluate profile flushing (OF-sEVs) OECs cows compared to...
Epigenetic modifications in the C-terminal domain of histones coordinate important events during early development including embryo genome activation (EGA) and cell differentiation. In this study, mRNA expression profile main lysine demethylases (KDMs) acting on 4 (H3K4), 9 (H3K9), 27 (H3K27) histone H3 was determined at pre-, post-EGA stages bovine porcine embryos produced by vitro fertilization (IVF) somatic nuclear transfer (SCNT). IVF embryos, abundance most KDMs revealed a bell-shaped...
In vivo- and in vitro-produced bovine embryos have different metabolic profiles differences gene transcription patterns. These also a distinct ability to establish sustain early pregnancies. Small extracellular vesicles (sEVs) are secreted by carry bioactive molecules, such as miRNAs. We hypothesize that vivo or hatched blastocysts on Day 9 the sEVs them miRNA profiles. To address this hypothesis, of both groups were placed vitro culture 7. After 48 h, embryo-conditioned media (eCM)...
Despite the advances in vitro embryo production (IVP) over years, technique still has limitations that need to be overcome. In cell cultures, it is already well established three-dimensional culture techniques are more physiological and similar vivo development. Liquid marble (LM) a system based on use of hydrophobic substance create microbioreactors. Thus, we hypothesized LM improves bovine oocyte maturation culture. experiment I, cumulus-oocyte complexes (COCs) were placed for 22h two...
Abstract We tested the hypothesis that biosensor capability of endometrium is mediated in part, by effect different cargo contained extracellular vesicles secreted conceptus during peri‐implantation period pregnancy. transferred Bos taurus embryos origin, vivo (high developmental potential (IV)), vitro (intermediate (IVF)), or cloned (low (NT)), into indicus recipients. Extracellular (EVs) recovered from Day 16 conceptus‐conditioned medium were characterized and their microRNA (miRNA)...
Abstract The first interactions among the embryo, endometrium, and corpus luteum are essential for pregnancy success. Small extracellular vesicles (sEVs) part of these interactions. We previously demonstrated that small from in vivo- or vitro-produced bovine embryos contain different miRNA cargos. Herein we show: (1) presence origin (in vivo vitro) blastocyst differentially reprograms endometrial transcriptional profiles; (2) explant (EE) cultured with vitro release contents, (3) luteal...
Intercellular communication is an essential mechanism for development and maintenance of multicellular organisms. Extracellular vesicles (EVs) were recently described as new players in the intercellular communication. EVs are double-membrane secreted by cells classified according to their biosynthesis, protein markers morphology. These extracellular contain bioactive materials such miRNA, mRNA, lipids. characteristics permit involvement different biological processes. Reproductive physiology...
Adiponectin is the most abundantly produced human adipokine with anti-inflammatory, anti-oxidative, and insulin-sensitizing properties. Evidence from in vitro studies has indicated that adiponectin a potential role reproduction because it reduces production of androstenedione bovine theca cells vitro. However, this effect on androgen not yet been observed vivo. The current study evaluated secretion oxidative stress parameters rodent model. Seven-week-old female Balb/c mice (n = 33),...
Background: Several studies have described an enhanced inflammatory status and oxidative stress balance disruption in women with polycystic ovary syndrome (PCOS). However, there is scarce information about redox markers the blood of androgenized animal models. Here, we evaluated serum/plasma marker metabolic parameter characteristics prenatal (PreN) postnatal (PostN) rat models PCOS. Materials methods: For PreN androgenization (n=8), 2.5 mg testosterone propionate was subcutaneously...
Abstract Orchestrated events, including extensive changes in epigenetic marks, allow a somatic nucleus to become totipotent after transfer into an oocyte, process termed nuclear reprogramming. Recently, several strategies have been applied order improve reprogramming efficiency, mainly focused on removing repressive marks such as histone methylation from the nucleus. Herein we used specific and non-toxic chemical probe UNC0638 inhibit catalytic activity of methyltransferases EHMT1 EHMT2....
In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. total, three pEFs lines reprogrammed, cultured at least 15 passages, characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous endogenous genes, immunofluorescence). Two piPSC further...
Interferon tau (IFNT) is the pregnancy recognition signal in ruminants and secreted by trophoblast cells. Paracrine action endometrium well established inhibiting luteolytic pulses of prostaglandin F2 alpha. Recently, endocrine was documented corpus luteum, blood cell liver. It hypothesized that conditioned medium (CM) obtained from days 7, 9 12 parthenogenetic embryos alters luteal gene expression. The aim to establish a bovine mixed culture evaluate cellular response associated interferon...
Several opportunities for embryo development, stem cell maintenance, fate, and differentiation have emerged using induced pluripotent cells (iPSCs). However, the difficulty in comparing bovine iPSCs (biPSCs) with embryonic (ESCs) was a challenge many years. Here, we reprogrammed fetal fibroblasts by transient expression of four transcription factors (Oct4, Sox2, Klf4, c-Myc, collectively termed "OSKM" factors) cultured iPSC medium, supplemented bFGF, bFGF2i, leukemia inhibitory factor (LIF),...
Abstract Purpose We hypothesized that vitamin D decreases rates of adenosine formation in human cutaneous melanoma cells through the inhibition extracellular 5′‐triphosphate breakdown, thereby affecting tumor cell viability. Therefore, objective this study was to explore mechanisms action 1α, 25‐dihydroxyvitamin D3 (1,25(OH) 2 D3) on activity and expression ectonucleotidases cells. Methods A line, SK‐Mel‐28, treated with 1 50 nM active metabolite over 24 hours, followed by determination...
Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows’ reproductive efficiency, cows were fed with different nutritional plans obtain animals high BER (HBER; Ad libitum diet) and moderate (MBER: 70% HBER group ingestion). To BER, weekly weighted evaluated for subcutaneous fat thickness insulin serum concentration along experimental period. At end period, submitted estrous synchronization artificial insemination. Animals slaughtered approximately 120 h after...
Insufficient epigenetic reprogramming is incompatible with normal development of embryos produced by somatic cell nuclear transfer (SCNT), but treatment histone deacetylases inhibitors (HDACi) enhances SCNT embryos. However, the mechanisms underpinning HDACi benefits in remain largely uncharacterized. We hypothesized that, addition to enhancing reprogramming, may promote expression genes not required for early To test this hypothesis, RNA synthesis was inhibited treating bovine...