A5024943500- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Viral Infections and Outbreaks Research
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- COVID-19 epidemiological studies
- Plant Virus Research Studies
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Poxvirus research and outbreaks
- interferon and immune responses
- Vaccine Coverage and Hesitancy
- Viral Infectious Diseases and Gene Expression in Insects
- Malaria Research and Control
- Hemoglobin structure and function
- Chronic Lymphocytic Leukemia Research
- vaccines and immunoinformatics approaches
- Neonatal Health and Biochemistry
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
World Bank
2025
National University of General San Martín
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2024
Universidad de Magallanes
2017-2024
Universidad Nacional de San Martín
2014-2024
Centro Científico Tecnológico - Tucumán
2022
Instituto Tecnológico de Chascomús
2018-2022
Yale University
2011-2013
Fundación Instituto Leloir
2005-2010
University of Buenos Aires
2002-2009
The mechanisms of RNA replication plus-strand viruses are still unclear. Here, we identified the first promoter element for synthesis described in a flavivirus. Using dengue virus as model, found that viral RdRp discriminates by specific recognition 5' named SLA. We demonstrated RNA-RNA interactions between and 3' end sequences genome enhance only presence an intact propose novel mechanism minus-strand which polymerase binds SLA at reaches site initiation via long-range interactions. These...
ABSTRACT Secondary and tertiary RNA structures present in viral genomes play essential regulatory roles during translation, replication, assembly of new particles. In the case flaviviruses, RNA-RNA interactions between 5′ 3′ ends genome have been proposed to be required for replication. We found that two elements at dengue virus interact vitro with high affinity. Visualization individual molecules by atomic force microscopy reveled physical interaction these results cyclization RNA. Using...
Massive vaccination offers great promise for halting the global COVID-19 pandemic. However, limited supply and uneven vaccine distribution create an urgent need to optimize strategies. We evaluate SARS-CoV-2-specific antibody responses after Sputnik V of healthcare workers in Argentina, measuring IgG anti-spike titers neutralizing capacity one two doses a cohort naive or previously infected volunteers. By 21 days receiving first dose vaccine, 94% participants develop spike-specific...
The plasticity of viral plus strand RNA genomes is fundamental for the multiple functions these molecules. Local and long-range – interactions provide scaffold interacting proteins translation, replication, encapsidation machinery. Using dengue virus as a model, we investigated relevance interplay between two alternative conformations genome during replication. Flaviviruses require cyclization synthesis. Here, define sequence present in 3′UTR that overlaps mutually exclusive structures. This...
Vaccines have been produced in record time for SARS-CoV-2, offering the possibility of halting global pandemic. However, inequalities vaccine accessibility different regions world create a need to increase international cooperation.
Abstract In the context of continuous emergence SARS-CoV-2 variants concern (VOCs), one strategy to prevent severe outcomes COVID-19 is developing safe and effective broad-spectrum vaccines. Here, we present preclinical studies a RBD vaccine derived from Gamma variant adjuvanted with Alum. The Gamma-adapted more immunogenic than Ancestral in terms inducing broader neutralizing antibodies. presents B-cell restricted epitopes induces higher proportion specific-B cells plasmablasts version....
Past studies have documented detrimental effects of the COVID-19 pandemic on learning and mental health preschool- school-age children. Few examined younger children's development, though this age group is extremely sensitive to economic shocks. We assessed exposure cognitive, language, motor development; behaviour; growth among toddlers in rural Bangladesh. estimated average differences between two repeated cross-sectional surveys children mothers living same villages. The first survey...
The potential of RNA viruses to adapt new environments relies on their ability introduce changes in genomes, which has resulted the recent expansion re-emergent viruses. Chikungunya virus is an important human pathogen transmitted by mosquitoes that, after 60 years exclusive circulation Asia and Africa, rapidly spread Europe Americas. Here, we examined evolution CHIKV different hosts uncovered host-specific requirements 3'UTR. Sequence repeats are conserved at 3'UTR but vary copy number...
BVDV causes one of the most economically important viral infections for cattle industry. The virus is able to cross placenta and infect fetus, leading birth persistently infected animals, which are reservoirs spread BVDV. occurrence persistent infection has hampered efficacy vaccination because it requires eliciting levels protection close sterilizing immunity prevent fetal infections. While prevents disease, can be detected if animals with neutralizing antibodies challenged virus. Virus...
It is well established that selective pressures in mosquito vectors impose population bottlenecks for arboviruses. Here, we used a CHIKV Caribbean lineage mutant carrying deletion the 3′ UTR to study host-virus interactions vivo epidemic vector Aedes aegypti . We found virus had delayed replication rate mosquitoes, which lengthened extrinsic incubation period (EIP) and reduced fitness relative wild-type virus. As result, displayed capacity cross anatomical barriers during infection cycle...
In this work, we evaluated recombinant receptor binding domain (RBD)-based vaccine formulation prototypes with potential for further clinical development. We assessed different formulations containing RBD plus alum, AddaS03, AddaVax, or the combination of alum and U-Omp19: a novel Brucella spp. protease inhibitor adjuvant. Results show that composed U-Omp19 as adjuvants has better performance: it significantly increased mucosal systemic neutralizing antibodies in comparison to antigen...
Vaccinia virus dissemination relies on the N-WASP–ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for formin FHOD1 and small GTPase Rac1 in vaccinia formation. depletion decreased number of CEVs forming tails impaired elongation rate formed tails. Recruitment to relied its binding domain addition FH2 domain. In agreement with previous studies showing that is activated by Rac1, was...
A Gamma Variant RBD-based aluminum hydroxide adjuvanted vaccine called ARVAC CG was selected for a first in human clinical trial. Healthy male and female participants (18-55 years old) with complete COVID-19-primary scheme were assigned to receive two intramuscular doses of either low-dose or high-dose CG. The primary endpoint safety. secondary objective humoral immunogenicity. Cellular immune responses studied as an exploratory objective. trial prospectively registered PRIISA.BA...
ABSTRACT Listeria monocytogenes is an intracellular pathogen that disseminates within the intestinal epithelium through acquisition of actin-based motility and formation plasma membrane protrusions project into adjacent cells. The resolution vacuoles from which escapes results in bacterial spread cell to cell. This dissemination process relies on mlp-actA-plcB operon, encodes ActA, a nucleation-promoting factor mediates motility, PlcB, phospholipase vacuole escape. Here we investigated role...
The worldwide expansion of chikungunya virus (CHIKV) into tropical and subtropical areas in the last 15 years has posed a currently unmet need for vaccines therapeutics. E2-E1 envelope glycoprotein complex binds receptors on host cell promotes membrane fusion during CHIKV entry, thus constituting an attractive target development antiviral drugs. In order to identify antivirals acting through inhibition function, our first approach was search amenable druggable sites within heterodimer. We...
Abstract Zika virus (ZIKV) is an emerging mosquito-borne flavivirus that causes severe outbreaks in human populations. ZIKV infection leads to the accumulation of small non-coding viral RNAs (known as sfRNAs) are crucial for evasion antiviral responses and pathogenesis. However, mechanistic understanding how sfRNAs function remains incomplete. Here, we use recombinant ZIKVs ribosome profiling infected cells show block translation genes. Mechanistically, demonstrate specific RNA structures...
Sequences and structures present at the 5' 3' UTRs of RNA viruses play crucial roles in initiation regulation translation, synthesis viral assembly. In dengue virus, as well other mosquito-borne flaviviruses, presence complementary sequences ends genome mediate long-range RNA-RNA interactions. Dengue virus displays two pairs (CS UAR) required for circularization viability. order to study molecular mechanism by which these interactions participate life cycle, we developed a replicon system....
Introduction: Cannabidiol (CBD), the main non-psychoactive cannabinoid of Cannabis sativa plant, is a powerful antioxidant compound that in recent years has increased interest due to causes effects wide range biological functions. Zika virus (ZIKV) transmitted mainly by Aedes aegypti mosquitoes, which neurological diseases, such as microcephaly and Guillain-Barre syndrome. Although frequency viral outbreaks recently, no vaccinations or particular chemotherapeutic treatments are available for...