A5011949298- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- HIV/AIDS Research and Interventions
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- HIV/AIDS oral health manifestations
- Pneumonia and Respiratory Infections
- Reproductive System and Pregnancy
- Congenital Diaphragmatic Hernia Studies
- Clusterin in disease pathology
- Mosquito-borne diseases and control
- Diabetes and associated disorders
- HIV/AIDS drug development and treatment
- Vaccine Coverage and Hesitancy
- T-cell and B-cell Immunology
- COVID-19 epidemiological studies
- Tracheal and airway disorders
- Immunodeficiency and Autoimmune Disorders
- Oral and gingival health research
- Neonatal Respiratory Health Research
- Influenza Virus Research Studies
- Reproductive tract infections research
- HIV-related health complications and treatments
Instituto de Investigaciones Biomédicas en Retrovirus y Sida
2015-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2025
Instituto Médico Río Cuarto
2014-2024
University of Buenos Aires
2013-2023
Instituto Mora
2021
Institute for Medical Research
2013
Universidade de São Paulo
2012
Universidad de Granada
1978-2008
Hôpital Xavier Arnozan
2008
Academia Nacional de Medicina
2006-2007
The relationship between pneumococcal conjugate vaccine-induced antibody responses and protection against community-acquired pneumonia (CAP) acute otitis media (AOM) is unclear. This study assessed the impact of ten-valent nontypable Haemophilus influenzae protein D vaccine (PHiD-CV) on these end points. primary objective was to demonstrate efficacy (VE) in a per-protocol analysis likely bacterial CAP (B-CAP: radiologically confirmed with alveolar consolidation/pleural effusion chest X-ray,...
Abstract Coronavirus infection in humans is usually associated to respiratory tract illnesses, ranging severity from mild life-threatening failure. The aryl hydrocarbon receptor (AHR) was recently identified as a host factor for Zika and dengue viruses; AHR antagonists boost antiviral immunity, decrease viral titers ameliorate Zika-induced pathology vivo. Here we report that activated by with different coronaviruses, potentially impacting immunity lung epithelial cells. Indeed, the analysis...
Abstract Inflammation in peripheral tissues is usually associated with the development of local acidosis; however, there are few studies aimed at analyzing influence acidosis on immune cells. We have shown previously that extracellular triggers human neutrophil activation, inducing a transient increase intracellular Ca2+ concentration, shape change response, up-regulation CD18 expression, and delay apoptosis. In this study, we analyzed signaling pathways responsible for activation. found...
Semen is the main vector for HIV-1 dissemination worldwide. It contains three major sources of infectious virus: free virions, infected leukocytes, and spermatozoa-associated virions. We focused on interaction with human spermatozoa dendritic cells (DCs). report that heparan sulfate expressed in plays an important role capture HIV-1. Spermatozoa-attached virus efficiently transmitted to DCs, macrophages, T cells. Interaction DCs not only leads transmission internalization but also results...
Abstract Although the development of an acidic tissue environment or acidosis is a hallmark inflammatory processes, few studies analyze effect extracellular pH on immune cells. We have previously shown that exposure murine dendritic cells (DCs) to 6.5 stimulates macropinocytosis and cross-presentation Ags by MHC class I molecules. report transient human DCs markedly increases expression HLA-DR, CD40, CD80, CD86, CD83, CCR7 improves T cell priming ability DCs. Incubation at results in...
Dengue virus (DENV) is the most important human transmitted by mosquitos. pathogenesis characterized a large induction of proinflammatory cytokines. This cytokine varies among four DENV serotypes (DENV1 to 4) and poses challenge for live vaccine design. Here, we identify viral mechanism limit NF-κB activation secretion protein NS5. Using proteomics, found that NS5 binds degrades host ERC1 antagonize activation, secretion, reduce cell migration. We degradation involves unique properties...
Abstract The C-type lectin receptor dendritic cell-specific ICAM-3–grabbing nonintegrin (DC-SIGN) is an important player in the recognition of pathogens by cells. A plethora including viruses, bacteria, parasites, and fungi are recognized DC-SIGN through both mannose fucose-containing glycans expressed on pathogen surface. In this study, we identified semen clusterin as a novel ligand. Semen clusterin, but not serum expresses extreme abundance blood-type Ags such Lex Ley, which excellent...
Vaccines have been produced in record time for SARS-CoV-2, offering the possibility of halting global pandemic. However, inequalities vaccine accessibility different regions world create a need to increase international cooperation.
Abstract In the context of continuous emergence SARS-CoV-2 variants concern (VOCs), one strategy to prevent severe outcomes COVID-19 is developing safe and effective broad-spectrum vaccines. Here, we present preclinical studies a RBD vaccine derived from Gamma variant adjuvanted with Alum. The Gamma-adapted more immunogenic than Ancestral in terms inducing broader neutralizing antibodies. presents B-cell restricted epitopes induces higher proportion specific-B cells plasmablasts version....
Abstract Previous studies have shown that histamine is able to modulate the function of dendritic cells (DCs). Histamine seems be required for normal differentiation DCs. Moreover, it capable stimulating chemotaxis immature DCs and promoting T CD4+ into a Th2 profile. In this study, we analyzed whether was endocytosis cross-presentation mediated by Our results show both functions are stimulated histamine. Endocytosis soluble HRP FITC-OVA OVA were markedly increased Interestingly, stimulation...
ABSTRACT Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is expressed by dendritic cells (DCs) at mucosal surfaces and appears to play an important role in the dissemination of human immunodeficiency virus type 1 (HIV-1) infection. DC-SIGN binds HIV-1 gp120 efficiently transmits T CD4 + cells, which become center viral replication. Semen represents main vector for worldwide. In present study we show that seminal plasma (SP), even when used very high...
Abstract Seminal plasma is not just a carrier for spermatozoa. It contains high concentrations of cytokines, chemokines, and other biological compounds that are able to exert potent effects on the immune system receptive partner. Previous studies have shown semen induces an acute inflammatory response at female genital mucosa after coitus. Moreover, it regulatory mechanisms allow fetus (a semiallograft) grow develop in uterus. The underlying these mechanisms, however, poorly understood. In...
Background A growing body of research shows a reciprocal regulation between the neural and immune systems. Acetylcholine (ACh) is most important parasympathetic neurotransmitter, increasing evidence indicates that it able to modulate response. Interestingly, in recent years, has become clear cells express non-neuronal cholinergic system, which stimulated course inflammatory processes. We have previously shown dendritic (DC) muscarinic receptors, as well enzymes responsible for synthesis...
Abstract Severe coronavirus disease 2019 (COVID-19) is associated with an overactive inflammatory response mediated by macrophages. Here, we analyzed the phenotype and function of neutrophils in patients COVID-19. We found that from severe COVID-19 express high levels CD11b CD66b, spontaneously produce CXCL8 CCL2, show a strong association platelets. Production correlated plasma concentrations lactate dehydrogenase D-dimer. Whole blood assays revealed clear immunoglobulin G (IgG) immune...
Although human airway epithelial cells are the main target of respiratory syncytial virus (RSV), it also infects immune cells, such as macrophages and B cells. Whether T permissive to RSV infection is unknown. We sought analyze permissiveness CD4+ infection. CD8+ from cord blood, healthy young children, adults were challenged by or cocultured with infected HEp-2 Infection, phenotype, cytokine production analyzed flow cytometry enzyme-linked immunosorbent assay. Expression antigens...
Abstract Background The true burden of lower respiratory tract infections (LRTIs) due to syncytial virus (RSV) remains unclear. This study aimed provide more robust, multinational data on RSV-LRTI incidence and in the first 2 years life. Methods prospective, observational cohort was conducted Argentina, Bangladesh, Canada, Finland, Honduras, South Africa, Thailand, United States. Children were followed for 24 months from birth. Suspected LRTIs detected via active (through regular contacts)...
Various case definitions of respiratory syncytial virus lower tract infection (RSV-LRTI) are currently proposed. We assessed the performance 3 clinical against World Health Organization definition recommended in 2015 (WHO 2015).In this prospective cohort study conducted 8 countries, 2401 children were followed up for 2 years from birth. Suspected LRTIs detected via active and passive surveillance, by in-person evaluation including single timepoint rate oxygen saturation (by pulse oximetry)...
A Gamma Variant RBD-based aluminum hydroxide adjuvanted vaccine called ARVAC CG was selected for a first in human clinical trial. Healthy male and female participants (18-55 years old) with complete COVID-19-primary scheme were assigned to receive two intramuscular doses of either low-dose or high-dose CG. The primary endpoint safety. secondary objective humoral immunogenicity. Cellular immune responses studied as an exploratory objective. trial prospectively registered PRIISA.BA...
There is limited evidence regarding the proportion of wheeze in young children attributable to respiratory syncytial virus lower tract infections (RSV-LRTI) occurring early life. This cohort study prospectively determined population risk (PAR) and percent (PAR%) 2-<6-year-old previously surveilled a primary for RSV-LRTI from birth their second birthday (RSV-LRTI<2Y).
Could seminal plasma clusterin play a role in the uptake of stress-damaged proteins by dendritic cells? Seminal clusterin, but not serum promotes cells via DC-SIGN. Clusterin is one major extracellular chaperones. It interacts with variety stressed to prevent their aggregation, guiding them for receptor-mediated endocytosis and intracellular degradation. The concentration semen almost 20-fold higher than that found serum, raising question about reproduction. No previous studies have analyzed...
BackgroundMost children and youth develop mild or asymptomatic disease during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, a very small number of patients suffer Coronavirus induced 2019 (COVID-19). The reasons underlying these different outcomes remain unknown.MethodsWe analyzed three cohorts: with infection (n=550), convalescent (n=138), MIS-C (multisystem inflammatory in children, n=42). IgG IgM antibodies to the spike protein SARS-CoV-2,...