Marjorie Cornejo Pontelli
A5076246462- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Viral Infections and Vectors
- Inflammasome and immune disorders
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Respiratory viral infections research
- COVID-19 and Mental Health
- Viral Infections and Outbreaks Research
- COVID-19 epidemiological studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacteriophages and microbial interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Parvovirus B19 Infection Studies
- SARS-CoV-2 detection and testing
- Plant Virus Research Studies
- Algal biology and biofuel production
- Antimicrobial Peptides and Activities
- Bat Biology and Ecology Studies
- Computational Drug Discovery Methods
- Dermatological and COVID-19 studies
- COVID-19 Impact on Reproduction
- Intensive Care Unit Cognitive Disorders
- Inflammation biomarkers and pathways
Washington University in St. Louis
2020-2024
Universidade de São Paulo
2015-2023
Ministry of Science, Technology and Innovation
2023
Universidade Federal de São Paulo
2022-2023
Avanti (United Kingdom)
2023
Universidade Federal do Ceará
2023
Secretaria da Saúde
2023
Universidade de Ribeirão Preto
2020-2022
Clinics Hospital of Ribeirão Preto
2020-2022
Universidade Federal de Mato Grosso do Sul
2022
Severe COVID-19 patients develop acute respiratory distress syndrome that may progress to cytokine storm syndrome, organ dysfunction, and death. Considering neutrophil extracellular traps (NETs) have been described as important mediators of tissue damage in inflammatory diseases, we investigated whether NETs would be involved pathophysiology. A cohort 32 hospitalized with a confirmed diagnosis healthy controls were enrolled. The concentration was augmented plasma, tracheal aspirate, lung...
Severe cases of COVID-19 are characterized by a strong inflammatory process that may ultimately lead to organ failure and patient death. The NLRP3 inflammasome is molecular platform promotes inflammation via cleavage activation key molecules including active caspase-1 (Casp1p20), IL-1β, IL-18. Although participation the in has been highly speculated, outcome disease unknown. Here we demonstrate activated response SARS-CoV-2 infection patients. Studying moderate severe patients, found PBMCs...
Although increasing evidence confirms neuropsychiatric manifestations associated mainly with severe COVID-19 infection, long-term dysfunction (recently characterized as part of "long COVID-19" syndrome) has been frequently observed after mild infection. We show the spectrum cerebral impact acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ranging from alterations in mildly infected individuals (orbitofrontal cortical atrophy, neurocognitive impairment, excessive fatigue and anxiety...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent of a major global outbreak tract disease known as Coronavirus Disease 2019 (COVID-19). SARS-CoV-2 infects mainly lungs and may cause several immune-related complications, such lymphocytopenia cytokine storm, which are associated with severity predict mortality. mechanism by infection result in immune system dysfunction still not fully understood. Here, we show that human CD4+ T helper cells, but CD8+ present blood...
Although SARS-CoV-2 severe infection is associated with a hyperinflammatory state, lymphopenia an immunological hallmark, and correlates poor prognosis in COVID-19. However, it remains unknown if circulating human lymphocytes monocytes are susceptible to infection. In this study, of peripheral blood mononuclear cells (PBMCs) was investigated both vitro vivo . We found that whole PBMCs from healthy donors productive virus progeny. Results revealed monocytes, as well B T lymphocytes, active...
Abstract Background The release of neutrophil extracellular traps (NETs) is associated with inflammation, coagulopathy, and organ damage found in severe cases COVID-19. However, the molecular mechanisms underlying NETs COVID-19 remain unclear. Objectives We aim to investigate role Gasdermin-D (GSDMD) pathway on development during Methods performed a single-cell transcriptome analysis public data bronchoalveolar lavage. Then, we enrolled 63 hospitalized patients moderate analyze blood lung...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with a hyperinflammatory state and lymphocytopenia, hallmark that appears as both signature prognosis of disease severity outcome. Although cytokine storm sustained inflammatory are commonly immune cell depletion, it still unclear whether direct SARS-CoV-2 cells could also play role in this scenario by harboring viral replication. We found monocytes, well B T lymphocytes, were susceptible to...
Abstract Although increasing evidence confirms neuropsychiatric manifestations associated mainly with severe COVID-19 infection, the long-term dysfunction has been frequently observed after mild infection. Here we show spectrum of cerebral impact SARS-CoV-2 infection ranging from alterations in mildly infected individuals (orbitofrontal cortical atrophy, neurocognitive impairment, excessive fatigue and anxiety symptoms) to acute damage confirmed brain tissue samples extracted orbitofrontal...
Abstract Severe COVID-19 patients develop acute respiratory distress syndrome that may progress to failure. These also cytokine storm syndrome, and organ dysfunctions, which is a clinical picture resembles sepsis. Considering neutrophil extracellular traps (NETs) have been described as an important factors of tissue damage in sepsis, we investigated whether NETs would be produced participate the lung damage. A cohort 32 hospitalized with confirmed diagnosis respective healthy controls were...
To assess whether high-dose coronavirus disease (COVID-19) convalescent plasma (CCP) transfusion may benefit patients with severe COVID-19, we conducted a multicenter randomized trial in Brazil. Patients COVID-19 who were within 10 days of initial symptom onset eligible. the CCP group received 3 daily doses (600 mL/d) addition to standard treatment; control treatment only. Primary outcomes death rates at 30 and 60 study randomization. Secondary ventilator-free hospital-free days. We enrolled...
The rapid spread of SARS-CoV-2 caused a global pandemic. Due to the lack treatment and vaccines, safety strategies must be found stop virus dissemination. objective this study was evaluate virucidal activity ozonated water, powerful oxidizing agent, against SARS-CoV-2. A special faucet source water at low concentration (0.2–0.8 ppm). At concentration, tests with SARS-Cov-2 in Vero CCL81 lineage showed two log10 reduction infectivity upon 1 min exposure comparison controls. It shows potential...
Vaccines have been produced in record time for SARS-CoV-2, offering the possibility of halting global pandemic. However, inequalities vaccine accessibility different regions world create a need to increase international cooperation.
Bats (Order: Chiroptera) harbor a high diversity of emerging pathogens presumably because their ability to fly and social behavior favor the maintenance, evolution, dissemination these pathogens. Until 2012, there was only one report presence Hantavirus in bats. Historically, it thought that viruses were harbored primarily by rodent insectivore small mammals. Recently, new species hantaviruses have been identified bats from Africa Asia continents expanding potential reservoirs range viruses....
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent of a major global outbreak tract disease known as disease-2019 (COVID-19). SARS-CoV-2 infects mainly lungs and may cause several immune-related complications, such lymphocytopenia cytokine storm, which are associated with severity predict mortality 1,2 . mechanism by infection result in immune system dysfunction still not fully understood. Here we show that human CD4 + T helper cells, but CD8 present blood...
Oropouche virus (OROV) is an emerging arbovirus in South and Central Americas with high spreading potential. OROV infection has been associated neurological complications genomic RNA detected cerebrospinal fluid from patients, suggesting its neuroinvasive Motivated by these findings, neurotropism neuropathogenesis of have investigated vivo murine models, which do not fully recapitulate the complexity human brain. Here we used slice cultures adult brains to investigate whether capable...
Significance Vaccines remain the best hope of curtailing SARS-CoV-2 transmission, morbidity, and mortality. Currently available vaccines require cold storage sophisticated manufacturing capacity, complicating their distribution, especially in less developed countries. We report a protein-based vaccine that directly specifically targets antigen-presenting cells. It consists Spike receptor-binding domain (Spike RBD ) fused to nanobody recognizes class II major histocompatibility complex...
Oropouche virus (OROV) infection of humans is associated with a debilitating febrile illness that can progress to meningitis or encephalitis. First isolated from forest worker in Trinidad and Tobago 1955, the arbovirus OROV has since been detected throughout Amazon basin an estimated 500,000 human infections over 60 years. Like other members family Peribunyaviridae, viral genome exists as 3 single-stranded negative-sense RNA segments. The medium-sized segment encodes glycoprotein complex...
Peribunyaviridae is a large family of RNA viruses with several members that cause mild to severe diseases in humans and livestock. Despite their importance public heath very little known about the host cell factors hijacked by these support assembly egress. Here we show Oropouche virus, member genus Orthobunyavirus causes frequent arboviral infection South America countries, involves budding virus particles toward lumen Golgi cisternae. As viral replication progresses, subcompartments become...
Abstract Severe cases of COVID-19 are characterized by a strong inflammatory process that may ultimately lead to organ failure and patient death. The NLRP3 inflammasome is molecular platform promotes inflammation via cleavage activation key molecules including active caspase-1 (Casp1p20), IL-1β IL-18. Although the participation in has been highly speculated, outcome disease unknown. Here we demonstrate activated response SARS-CoV-2 infection it COVID-19, influencing clinical disease....
Oropouche orthobunyavirus (OROV) is an emerging arbovirus with a high potential of dissemination in America. Little known about the role peripheral blood mononuclear cells (PBMC) response during OROV infection humans. Thus, to evaluate human leukocytes susceptibility, permissiveness and immune infection, we applied RNA hybridization, qRT-PCR cell-based assays quantify viral antigens, genome, antigenome gene expression different cells. First, observed replication lineages as THP-1 monocytes,...
The TIGIT+FOXP3+Treg subset (TIGIT+Tregs) exerts robust suppressive activity on cellular immunity and predisposes septic individuals to opportunistic infection. We hypothesized that TIGIT+Tregs could play an important role in intensifying the COVID-19 severity hampering defense against nosocomial infections during hospitalization. Herein we aimed verify association between levels of with mechanical ventilation requirement, fatal outcome, bacteremia were immunophenotyped by flow cytometry...
Our ability to genetically manipulate living organisms is usually constrained by the efficiency of genetic tools available for system interest. In this report, we present design, construction and characterization a set four new modular vectors, pHsal series, engineering Halobacterium salinarum, model halophilic archaeon widely used in systems biology studies. The shuttle vectors are organized modules: (i) E. coli's specific part, containing ColE1 origin replication an ampicillin resistance...
SARS-CoV-2 uses the receptor binding domain (RBD) of its spike protein to recognize and infect host cells by cell surface angiotensin converting enzyme 2 (ACE2). The ACE2 is composed peptidase (PD), collectrin-like domain, transmembrane short cytoplasmic may exist as a dimer on surface. RBD site located atop PD, but involvement other domains in virus infection uncertain. We found that PD alone, whether anchored membrane via glycosylphosphatidylinositol anchor or attached another protein,...