A5086104883- Immune cells in cancer
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Pain Mechanisms and Treatments
- PARP inhibition in cancer therapy
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Treatment and Pharmacology
- Inflammasome and immune disorders
- Cell Adhesion Molecules Research
- Oral health in cancer treatment
- Cancer Cells and Metastasis
- Cancer therapeutics and mechanisms
- IL-33, ST2, and ILC Pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Venomous Animal Envenomation and Studies
- Essential Oils and Antimicrobial Activity
- Sepsis Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Estrogen and related hormone effects
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Neutropenia and Cancer Infections
- Natural product bioactivities and synthesis
- Vascular anomalies and interventions
Brigham and Women's Hospital
2023-2025
Harvard University
2024
Universidade de São Paulo
2018-2023
Universidade de Ribeirão Preto
2019-2022
Clinics Hospital of Ribeirão Preto
2022
Universidade Federal do Ceará
2014-2021
Bipar
2021
Universidade de Fortaleza
2017
Universidade Federal da Paraíba
2016
Universidade Federal do Vale do São Francisco
2011-2015
Abstract Paclitaxel is an antineoplastic agent widely used to treat several solid tumor types. The primary mechanism of action paclitaxel based on microtubule stabilization inducing cell-cycle arrest. Here, we use models show that not only induces arrest, but also promotes antitumor immunity. In vitro, reprogrammed M2-polarized macrophages the M1-like phenotype in a TLR4-dependent manner, similarly LPS. modulated tumor-associated macrophage (TAM) profile mouse breast and melanoma tumors;...
Neutrophil extracellular traps (NETs) are innate defense mechanisms that also implicated in the pathogenesis of organ dysfunction. However, role NETs pediatric sepsis is unknown.Infant (2 weeks old) and adult (6 mice were submitted to by intraperitoneal (i.p.) injection bacteria suspension or lipopolysaccharide (LPS). infiltration, bacteremia, injury, concentrations cytokine, NETs, DNase plasma measured. Production reactive oxygen nitrogen species release neutrophils evaluated. To...
The inflammasomes control the bioactivity of pro-inflammatory cytokines interleukin (IL)-1 family. inflammasome assembled by NLRP3 has been predominantly studied in homogeneous cell populations vitro, neglecting influence cellular interactions that occur vivo. Here, we show platelets boost capacity human macrophages and neutrophils are critical for IL-1 production monocytes. Platelets license transcription, thereby enhancing ASC oligomerization, caspase-1 activity, IL-1β secretion. vivo,...
Abstract Background The release of neutrophil extracellular traps (NETs) is associated with inflammation, coagulopathy, and organ damage found in severe cases COVID-19. However, the molecular mechanisms underlying NETs COVID-19 remain unclear. Objectives We aim to investigate role Gasdermin-D (GSDMD) pathway on development during Methods performed a single-cell transcriptome analysis public data bronchoalveolar lavage. Then, we enrolled 63 hospitalized patients moderate analyze blood lung...
We aimed to investigate the modulating effect of α-phellandrene on neutrophil migration and mast cell degranulation processes.Male Wistar rats or Swiss mice were treated p.o. with vehicle (3% Tween 80, p.o.), (50, 100, 200mg/kg, dexamethasone (0.5mg/kg, p.o.) 1h before carrageenan injection. Then, in 6-day-old air pouches peritoneal cavities. The leukocyte rolling adhesion measured real time assessed by intravital microscopy. ELISA was used detect TNF-α IL-6 lavage. Compound 48/80-induced...
Intestinal mucositis is a common side effect of irinotecan-based anticancer regimens. Mucositis causes cell damage, bacterial/endotoxin translocation and production cytokines including IL–1 IL–18. These molecules toll-like receptors (TLRs) activate signaling pathway that involves the Myeloid Differentiation adaptor protein, MyD88, whose role in intestinal unknown. Then, we evaluated involvement TLRs MyD88 pathogenesis irinotecan-induced mucositis. MyD88-, TLR2- or TLR9-knockout mice C57BL/6...
Myrtenol is a bicyclic monoterpene with anti-inflammatory properties. However, the mechanisms involved are partially unknown. Here, we investigated effect of myrtenol during experimental chronic arthritis and possible modulating activity oxidative stress neutrophil migration. Complete Freund's Adjuvant (CFA)-sensitized rats were treated vehicle (1 mL/kg, po), (12.5, 25 or 50 mg/kg, indomethacin (10 po) dexamethasone (0.4 mg/kg) followed by intra-articular injection CFA (0.5 mg/mL, μL per...
Colorectal cancer (CRC) is a disease with high incidence and mortality. Colonoscopy gold standard among tests used for CRC traceability. However, serious complications, such as colon perforation, may occur. Non-invasive diagnostic procedures are an unmet need. We aimed to identify plasma microRNA (miRNA) signature detection. Plasma samples were obtained from subjects (n = 109) at different stages of colorectal carcinogenesis. The patients stratified into non-cancer (27 healthy volunteers, 17...
Local tissue reactions provoked by Bothrops venoms are characterized edema, hemorrhage, pain, and inflammation; however, the mechanisms of damage vary depending upon species snake. Here, we investigated involved in local inflammatory response induced jararacussu venom (BjcuV). Female Swiss mice were injected with either saline, BjcuV (0.125–8 μg/paw) or loratadine (an H1 receptor antagonist), compound 48/80 (for mast cell depletion), capsaicin C-fiber desensitization), infliximab anti-TNF-α...
Background and Purpose Intestinal mucositis is a common side‐effect of irinotecan‐based cancer chemotherapy regimens. This associated with cytokine activation NO synthesis. Production IL ‐18 up‐regulated in patients suffering from inflammatory bowel disease. Therefore, we have investigated the role pathogenesis irinotecan‐induced intestinal mucositis. Experimental Approach Wild type (WT), or caspase‐1 knockout mice were treated either saline irinotecan (60 mg·kg −1 per 4 days, i.p.) binding...
Annona vepretorum Mart. (Annonaceae) is a native tree from Caatinga (Brazilian Northeastern savanna biome), popularly known as “araticum” and “pinha da Caatinga”. In this study, we investigated the effects of crude ethanolic extract (Av-EtOH) in models pain inflammation rodents. The evaluation antinociceptive activity was carried out by acetic acid-induced writhing, formalin, hot plate tail flick tests, while paw edema induced carrageenan or histamine, leukocyte migration to peritoneal...
Abstract Background Although the literature shows that an increase in both number and suppressive function of CD4+forkhead box P3 (FOXP3)+ T-regulatory cells (Tregs) during sepsis contributes to immunosuppressed state, little is known about identity these cells. Methods Using mouse model cecal ligation puncture (CLP), we analyzed frequency molecular signature T-cell immunoglobulin ITIM domain (TIGIT)+ TIGIT− Treg subsets, using flow cytometry quantitative polymerase chain reaction. In...
Abstract Background: Poly (ADP-Ribose) polymerase inhibitors (PARPi) have improved outcomes for BRCA-associated triple negative breast cancer (TNBC); however, resistance develops, resulting in lack of durable responses. Our previous work demonstrated that PARPi activates the cGAS/STING pathway inducing recruitment anti-tumor CD8+ T-cells, which are required tumor clearance. These studies led to clinical trials testing plus immune checkpoint blockade (ICB). However, + ICB is not superior...
T cells are sparse in hormone receptor-positive (HR+) breast cancer, potentially due to limited antigen presentation, but the mechanisms remain unclear. This study aimed elucidate how estrogen receptor (ER) signaling (ERS) modulates immune pathways and tumor microenvironment using bulk single-cell RNA sequencing (scRNA-seq) data spatial analysis via multiplexed cyclic immunofluorescence (CyCIF). Analysis of HR+/HER2- patients from TCGA METABRIC datasets identified co-expressed gene modules...
Bothropic venoms cause intense local damage, pain, edema, and myonecrosis. Morus nigra L. (Moraceae) has several uses in folk medicine can be a promising candidate for the treatment of inflammatory disorders.The present study aims to evaluate anti-inflammatory antinociceptive effects ethanolic extract (Mn-EtOH) on paw lesions induced by Bothrops jararacussu snake venom (BjcuV) mice.UV-vis absorption BjcuV was evaluated. A phytochemical performed, which led isolation characterization three...
Abstract Cytotoxic agents synergize with immune checkpoint inhibitors and improve outcomes for patients several cancer types. Nonetheless, a parallel increase in the incidence of dose-limiting side effects, such as peripheral neuropathy, is often observed. Here, we investigated role programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) axis modulation paclitaxel-induced neuropathic pain. We found that human mouse neural tissues, including dorsal root ganglion (DRG), expressed...
Abstract Background and Purpose Neutrophil overstimulation plays a crucial role in tissue damage during severe infections. Because pathogen‐derived neuraminidase (NEU) stimulates neutrophils, we investigated whether host NEU can be targeted to regulate the neutrophil dysregulation observed Experimental Approach The effects of inhibitors on lipopolysaccharide (LPS)‐stimulated neutrophils from healthy donors or COVID‐19 patients were determined by evaluating shedding surface sialic acids, cell...