A5011563848- Inflammasome and immune disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Psoriasis: Treatment and Pathogenesis
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- COVID-19 Clinical Research Studies
- interferon and immune responses
- Autoimmune and Inflammatory Disorders Research
- Blood disorders and treatments
- Herbal Medicine Research Studies
- Phytochemistry Medicinal Plant Applications
- Diabetes and associated disorders
- Bee Products Chemical Analysis
- Atherosclerosis and Cardiovascular Diseases
- Biochemical and Molecular Research
- Toxin Mechanisms and Immunotoxins
- Sex and Gender in Healthcare
- Adenosine and Purinergic Signaling
- Plant Toxicity and Pharmacological Properties
- Dermatology and Skin Diseases
- Spinal Cord Injury Research
- Immunodeficiency and Autoimmune Disorders
- Pancreatic function and diabetes
- Neonatal Health and Biochemistry
Universidade de São Paulo
2019-2025
Johannes Gutenberg University Mainz
2023-2025
Institute of Medical Microbiology and Hygiene
2023-2025
University Medical Center of the Johannes Gutenberg University Mainz
2023-2025
Universidade de Ribeirão Preto
2022
Bipar
2021
Universidade Estadual do Ceará
2012-2017
Th17 cell differentiation and pathogenicity depend on metabolic reprogramming inducing shifts toward glycolysis. Here, we show that the pyruvate kinase M2 (PKM2), a glycolytic enzyme required for cancer proliferation tumor progression, is key factor mediating autoimmune inflammation. We found PKM2 highly expressed throughout of cells in vitro during experimental encephalomyelitis (EAE) development. Strikingly, not proliferative capacity cells. However, T cell-specific deletion impairs...
Abstract Background The release of neutrophil extracellular traps (NETs) is associated with inflammation, coagulopathy, and organ damage found in severe cases COVID-19. However, the molecular mechanisms underlying NETs COVID-19 remain unclear. Objectives We aim to investigate role Gasdermin-D (GSDMD) pathway on development during Methods performed a single-cell transcriptome analysis public data bronchoalveolar lavage. Then, we enrolled 63 hospitalized patients moderate analyze blood lung...
Macrophages play a crucial role in immune responses and undergo metabolic reprogramming to fulfill their functions. The tetramerization of the glycolytic enzyme pyruvate kinase M2 (PKM2) induces production anti-inflammatory cytokine interleukin (IL)-10 vivo, but underlying mechanism remains elusive. Here, we report that PKM2 activation with pharmacological agent TEPP-46 increases IL-10 LPS-activated macrophages by reprogramming, leading release ATP from glycolysis. effect is abolished...
Abstract Neutrophils rely predominantly on glycolytic metabolism for their biological functions, including reactive oxygen species (ROS) production. Although pyruvate kinase M2 (PKM2) is a enzyme known to be involved in metabolic reprogramming and gene transcription many immune cell types, its role neutrophils remains poorly understood. Here, we report that PKM2 regulates ROS production microbial killing by neutrophils. Zymosan-activated showed increased cytoplasmic expression of PKM2....
Psoriasis is an inflammatory skin disease characterized by keratinocyte proliferation and cell infiltration induced IL-17. However, the molecular mechanism through which IL-17 signaling in keratinocytes triggers inflammation remains not fully understood. Pyruvate kinase M2 (PKM2), a glycolytic enzyme, has been shown to have non-metabolic functions. Here, we report that PKM2 mediates IL-17A triggering psoriatic inflammation. We find high expression of epidermis patients mice undergoing...
External and intrinsic factors regulate the transcriptional profile of T helper 17 (TH17) cells, thereby affecting their pathogenic potential revealing context-dependent plasticity. The stimulator interferon genes (STING), a component intracellular DNA-sensing pathway, triggers immune responses but remains largely unexplored in cells. Here, we describe an role STING limiting TH17 cell program. We demonstrate that non-pathogenic cells express higher levels than those activated under...
The dysregulated host response to infections can lead sepsis, a complex disease characterized by spectrum of clinical phenotypes. Using scRNA-seq, we analyzed the immune cell survived and non-survived CLP-septic mice gain insights into immunological mechanisms which neutrophils contribute hyperinflammatory phenotype. Our findings reveal that exhibit increased frequencies immature CXCR4+ PD-L1+ in bloodstream, accompanied an accumulation trafficking-specific lungs. IFN-gamma LPS promote PD-L1...
Sex hormone-induced cardiovascular events are important undesirable effects in transgender people under GAHT. Studies addressing the impact of GAHT will certainly contribute to improve healthcare services offered this population. Our study showing that vascular dysfunction, via Th17 cell-related mechanisms, precedes increased blood pressure induced by testosterone a mouse model, reveals potential mechanisms involved GAHT-related and may provide new markers/targets for clinical practices...
Vatairea guianensis seeds, a typical plant from the Brazilian Amazon region that belongs to Dalbergieae tribe, possess lectin was isolated by precipitation with solid ammonium sulfate followed guar gum affinity chromatography. This named VGL. The V. strongly agglutinated rabbit erythrocytes and inhibited d-galactose d-galactose-derived sugars, especially N-acetyl-d-galactosamine. VGL has been shown be stable protein, maintaining its hemagglutinating activity after incubation at wide range of...
Ximenia americana L., Olacaceae, barks are utilized in folk medicine as analgesic and anti-inflammatory. The objective was to evaluate the toxicity antinociceptive effect of polysaccharides rich fractions from X. barks. were obtained by extraction with NaOH, followed precipitation ethanol fractionation ion exchange chromatography. They administered i.v. or p.o. before nociception tests (writhing, formalin, carragenan-induced hypernociception, hot plate), during 14 days for assay. total...
Nucleotide oligomerization domain (NOD)-like receptor-12 (NLRP12) has emerged as a negative regulator of inflammation. It is well described that the Th17 cell population increases in patients with early Rheumatoid Arthritis (RA), which correlates disease activity. Here, we investigated role NLRP12 differentiation cells and development experimental arthritis, using antigen-induced arthritis (AIA) murine model. We found Nlrp12-/- mice develop severe characterized by an exacerbated...
The development of neuropathic pain after peripheral nerve injury involves neuroimmune-glial interactions in the spinal cord. However, whether depends on infiltration immune cells, such as monocytes, into cord parenchyma damage remains unclear. Here, we used a combination different techniques transgenic reporter mouse (Cx3cr1GFP/+ and Ccr2RFP/+ mice), bone marrow chimeric mice, parabiosis to investigate this issue spared (SNI) model. Herein, provided robust evidence that, although microglial...
Abstract The release of neutrophil extracellular traps (NETs) is associated with inflammation, coagulopathy, and organ damage found in severe cases COVID-19. However, the molecular mechanisms underlying NETs COVID-19 remain unclear. Using a single-cell transcriptome analysis we observed that expression GSDMD inflammasome-related genes were increased neutrophils from patients. Furthermore, high was structures lung tissue activation requires live SARS-CoV-2 occurs after infection via ACE2...