A5080132490- Inflammasome and immune disorders
- interferon and immune responses
- Bone health and treatments
- Prostate Cancer Treatment and Research
- Heme Oxygenase-1 and Carbon Monoxide
- Radiopharmaceutical Chemistry and Applications
- Herpesvirus Infections and Treatments
- Influenza Virus Research Studies
- Microbial Metabolism and Applications
- Poxvirus research and outbreaks
- RNA modifications and cancer
- Cancer Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Gout, Hyperuricemia, Uric Acid
- Management of metastatic bone disease
- Biochemical and biochemical processes
- TiO2 Photocatalysis and Solar Cells
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
University of Bonn
2021-2024
University Hospital Bonn
2023
University Hospital Cologne
2018-2020
University of Cologne
2019-2020
Heinrich Heine University Düsseldorf
2020
Düsseldorf University Hospital
2020
Universiti Malaysia Sarawak
2020
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread, with devastating consequences. For passive immunization efforts, nanobodies have size and cost advantages over conventional antibodies. In this study, we generated four neutralizing that target the receptor binding domain of SARS-CoV-2 spike protein. We used x-ray crystallography cryo-electron microscopy define two distinct epitopes. On basis these structures, engineered multivalent more...
Inflammasomes integrate cytosolic evidence of infection or damage to mount inflammatory responses. The inflammasome sensor NLRP1 is expressed in human keratinocytes and coordinates inflammation the skin. We found that diverse stress signals induce assembly by activating MAP kinase p38: While ribotoxic response UV microbial molecules exclusively activates p38 through MAP3K ZAKα, with arthropod-borne alphaviruses, including Semliki Forest Chikungunya virus, ZAKα potentially other MAP3K....
Orthomyxo- and bunyaviruses steal the 5' cap portion of host RNAs to prime their own transcription in a process called "cap snatching." We report that RNA modification by 2'-O-ribose methyltransferase 1 (MTr1) is essential for initiation influenza A B virus replication, but not other cap-snatching viruses. identified with silico compound screening functional analysis derivative natural product from Streptomyces, trifluoromethyl-tubercidin (TFMT), inhibits MTr1 through interaction at its...
Multiple omics analyzes of Vaccinia virus (VACV) infection have defined molecular characteristics poxvirus biology. However, little is known about the monkeypox (mpox) (MPXV) in humans, which has a different disease manifestation despite its high sequence similarity to VACV. Here, we perform an in-depth multi-omics analysis transcriptome, proteome, and phosphoproteome signatures MPXV-infected primary human fibroblasts gain insights into virus-host interplay. In addition expected...
Abstract Inflammasome activation results in the cleavage of gasdermin D (GSDMD) by pro-inflammatory caspases. The N-terminal domains (GSDMD NT ) oligomerize and assemble pores penetrating target membrane. As methods to study pore formation living cells are insufficient, order conformational changes, oligomerization, membrane insertion remained unclear. We have raised nanobodies (VHHs) against human GSDMD find that cytosolic expression VHH GSDMD-1 GSDMD-2 prevents oligomerization pyroptosis....
Inflammasomes are signaling complexes that coordinate inflammation by inducing rapid cytokine secretion and protect against invading pathogens initiating death of infected cells. Yet, current methods do not allow us to specifically monitor inflammasome activation in real-time at endogenous protein levels. To overcome this shortcoming, we developed characterized a novel fluorescent reporter visualizes assembly reports on the recruitment effector caspase-1 without impairing downstream...
Summary Inflammasomes integrate cytosolic evidence of infection or damage to mount inflammatory responses. The inflammasome sensor NLRP1 is expressed in human keratinocytes and coordinates inflammation the skin. We found that diverse stress signals converge on activation p38 kinases initiate assembly: UV irradiation microbial molecules ribotoxic response critically relied MAP3 kinase ZAKα activate ultimately NLRP1. Infection with insect-transmitted alphaviruses, including Semliki Forest,...