A5009605016- RNA modifications and cancer
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- COVID-19 Clinical Research Studies
- RNA Research and Splicing
- interferon and immune responses
- X-ray Diffraction in Crystallography
- vaccines and immunoinformatics approaches
- Crystallization and Solubility Studies
- PARP inhibition in cancer therapy
- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- Chromosomal and Genetic Variations
- Poxvirus research and outbreaks
- Metal complexes synthesis and properties
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Long-Term Effects of COVID-19
- RNA Interference and Gene Delivery
- Magnetism in coordination complexes
- Monoclonal and Polyclonal Antibodies Research
- Crystal structures of chemical compounds
- Mosquito-borne diseases and control
- Respiratory viral infections research
- Cytokine Signaling Pathways and Interactions
Technical University of Munich
2019-2024
Helmholtz Zentrum München
2021
Max Planck Institute of Biochemistry
2019
Summary The global emergence of SARS-CoV-2 urgently requires an in-depth understanding molecular functions viral proteins and their interactions with the host proteome. Several individual omics studies have extended our knowledge COVID-19 pathophysiology 1–10 . Integration such datasets to obtain a holistic view virus-host define pathogenic properties is limited by heterogeneity experimental systems. We therefore conducted concurrent multi-omics study SARS-CoV. Using state-of-the-art...
Multiple omics analyzes of Vaccinia virus (VACV) infection have defined molecular characteristics poxvirus biology. However, little is known about the monkeypox (mpox) (MPXV) in humans, which has a different disease manifestation despite its high sequence similarity to VACV. Here, we perform an in-depth multi-omics analysis transcriptome, proteome, and phosphoproteome signatures MPXV-infected primary human fibroblasts gain insights into virus-host interplay. In addition expected...
The SARS-CoV-2 pandemic imposed a large burden on health and society. Therapeutics targeting different components processes of the viral infection replication cycle are being investigated, particularly to repurpose already approved drugs. Spike protein is an important target for both vaccines therapeutics. Insights into mechanisms spike-ACE2 binding cell fusion could support identification compounds with inhibitory effects. Here, we demonstrate that integrity disulfide bonds within...
Abstract The SARS‐CoV‐2 infection cycle is a multistage process that relies on functional interactions between the host and pathogen. Here, we repurposed antiviral drugs against both viral enzymes to pharmaceutically block methylation of RNA 2'‐O‐ribose cap needed for immune escape. We find methyltransferase MTr1 can compensate loss NSP16 in facilitating virus replication. Concomitant inhibition efficiently suppresses Using silico target‐based drug screening, identify bispecific MTr1/NSP16...
Human respiratory syncytial virus (RSV) is a common cause of lower tract infections in the pediatric, elderly, and immunocompromised individuals. RSV non-structural protein NS1 known cytosolic immune antagonist, but how modulates host responses remains poorly defined. Here, we observe partitioning into nucleus RSV-infected cells, including human airway epithelium. Nuclear coimmunoprecipitates with Mediator complex chromatin associated. Chromatin-immunoprecipitation demonstrates enrichment...
Changes of mRNA 3'UTRs by alternative polyadenylation (APA) have been associated to numerous pathologies, but the mechanisms and consequences often remain enigmatic. By combining transcriptomics, proteomics recombinant viruses we show that all tested strains IAV, including A/PR/8/34(H1N1) (PR8) A/Cal/07/2009 (H1N1) (Cal09), cause APA. We mapped effect highly conserved glycine residue at position 184 (G184) viral non-structural protein 1 (NS1). Unbiased mass spectrometry-based analyses...
The response of the adaptive immune system is augmented by multimeric presentation a specific antigen, resembling viral particles. Several vaccines have been designed based on natural or protein scaffolds, which exhibited potent to antigens; however, antibodies are also generated against scaffold, may impair subsequent vaccination. In order compare polypeptide scaffolds different size and oligomerization state with respect their efficiency, including anti-scaffold immunity, we compared...
The spatial organisation of RNA condensates is fundamental for understanding basic cellular functions, but may also provide pivotal insights into diseases. One the major challenges to role lack technologies map condensate-scale protein architecture at subcompartmental or nanoscale resolution. To address this, we introduce HCR-Proxy, a proximity labelling technique that couples Hybridization Chain Reaction (HCR)-based signal amplification with in situ biotinylation (Proxy), enabling proteomic...
Cellular response to environmental challenges requires immediate and precise regulation of transcriptional programs. During viral infections, this includes the expression antiviral genes that are essential combat pathogen. Transcribed mRNAs bound escorted cytoplasm by cap-binding complex (CBC). We recently identified a protein consisting NCBP1 NCBP3 that, under physiological conditions, has redundant function canonical CBC, NCBP2. Here, we provide evidence is mount appropriate response....
Human endogenous retrovirus (HERVs), normally silenced by methylation or mutations, can be reactivated multiple environmental factors, including infections with exogenous viruses. In this work, we investigated the transcriptional activity of HERVs in human A549 cells infected two wild-type (PR8M, SC35M) and one mutated (SC35MΔNS1) strains Influenza A virus (IAVs). We found that majority differentially expressed (DEHERVS) genes (DEGs) were up-regulated cells, most significantly enriched...
The embryonic progression from naïve to primed pluripotency is accompanied by the rapid decay of pluripotency-associated mRNAs and a concomitant radical morphogenetic sequence epiblast polarization, rosette formation lumenogenesis. mechanisms triggering linking these events remain poorly understood. Guided machine learning metabolic RNA sequencing, we identified binding proteins (RBPs), especially LIN28A, as primary mRNA factors. Using mRNA-RBP interactome capture, revealed dramatic increase...