A5002939277- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Advanced Fluorescence Microscopy Techniques
- Acute Myeloid Leukemia Research
- Tissue Engineering and Regenerative Medicine
- Escherichia coli research studies
- Genetic Neurodegenerative Diseases
- Inflammasome and immune disorders
- T-cell and B-cell Immunology
- Bacterial Genetics and Biotechnology
- Antimicrobial Peptides and Activities
- IL-33, ST2, and ILC Pathways
- Gene Regulatory Network Analysis
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Ubiquitin and proteasome pathways
- Lipid Membrane Structure and Behavior
- Mast cells and histamine
- Immunotherapy and Immune Responses
National Institute of Chemistry
2014-2025
Flagellin is a wide-spread bacterial virulence factor sensed by the membrane-bound Toll-like receptor 5 (TLR5) and intracellular NAIP5/NLRC4 inflammasome receptor. TLR5 recognizes conserved region within D1 domain of flagellin, crucial for interaction between subunits in flagellum motility. While it known that deletion D0 which lines interior flagella, also completely abrogates activation TLR5, its functional role remains unknown. Using protein fusion strategy, we propose stabilization an...
Abstract The CRISPR/Cas system has emerged as a powerful and versatile genome engineering tool, revolutionizing biological biomedical sciences, where an improvement of efficiency could have strong impact. Here we present strategy to enhance gene editing based on the concerted action Cas9 exonuclease. Non-covalent recruitment exonuclease Cas9/gRNA complex via genetically encoded coiled-coil domains, termed CCExo, recruited cleavage site robustly increased knock-out due progressive DNA strand...
The response of the adaptive immune system is augmented by multimeric presentation a specific antigen, resembling viral particles. Several vaccines have been designed based on natural or protein scaffolds, which exhibited potent to antigens; however, antibodies are also generated against scaffold, may impair subsequent vaccination. In order compare polypeptide scaffolds different size and oligomerization state with respect their efficiency, including anti-scaffold immunity, we compared...
IL-33 is involved in allergic processes by promoting the release of various mast cell (MC) chemokines, including CCL2. However, it yet unclear which specific type primarily responsible for producing CCL2 during acute reactions. This study aims to investigate role production cells and assess effect MC-derived on basophil migration endothelial permeability. Human blood-derived MCs (hMCs) were generated from peripheral blood precursors, passively sensitized with IgE, treated IL-33, stimulated...
Toll-like receptor 5 (TLR5) is a of the innate immune system that recognizes flagellin from certain bacterial species and triggers an inflammatory response. The Salmonella dublin in complex with zebrafish TLR5 has been crystallized previously. In present study, we extrapolate structure this using structure-guided mutagenesis to determine recognition modes human mouse receptors demonstrate species-specific differences recognition. general, mode can be said more robust comparison receptor....
Abstract Background Forkhead box P3 + ( FOXP3 ) regulatory T cells (Tregs) are a subset of lymphocytes, critical for the maintenance immune homeostasis. Loss-of-function mutations gene in animal models and humans results loss differentiation potential into Treg responsible several immune-mediated inflammatory diseases. Strategies increasing expression represent approach to increase pool Tregs within lymphocyte population may be employed therapies diverse autoimmune conditions. In present...
Abstract TLRs sense conserved and essential molecular components of microbes that invade multicellular organisms. The wide range TLR agonists, differing in size shape, is recognized either through a single or pair binding sites on the ectodomains TLRs. TLR5 recognizes bacterial flagellin two distinct ectodomain, first facilitating primary second guiding receptor dimerization necessary for signaling. regions by encompass key functional within D1 domain flagellin, which also required assembly...
Abstract Effective and safe vaccines against SARS-CoV-2 are highly desirable to prevent casualties societal cost caused by Covid-19 pandemic. The receptor binding domain (RBD) of the surface-exposed spike protein represents a suitable target for induction neutralizing antibodies upon vaccination. Small antigens typically induce weak immune response while particles measuring tens nanometers efficiently presented B cell follicles subsequently follicular germinal center cells in draining lymph...
The considerable potential of engineered cells compels the development strategies for stringent control gene expression. A promising approach is introduction a premature stop codon (PTC) into selected that expressed only in presence noncanonical amino acids through nonsense suppression. Here, different amber PTC readthrough mammalian were tested. use tRNA synthetase together with TAG codon‐specific achieved depending on addition acid (4‐benzoyl‐ L ‐phenylalanine; Bpa). While single...
<h3>Background</h3> Chronic myeloid leukemia (CML) is a myeloproliferative neoplastic disease, occurring in 1 to 2 cases per 100.000 adults, which accounts this type of cancer for approximately 15% newly diagnosed adult patients. The diagnosis based upon the genetic translocation between t(9;22)(q34;q11.2), resulting formation Philadelphia fusion chromosome, coding BCR-ABL1 oncoprotein. life-long treatment relies on using tyrosine kinase inhibitors (TKIs). In some cases, patients develop...
Abstract Chronic myeloid leukemia (CML) is a myeloproliferative neoplastic disease, occurring in 1 to 2 cases per 100.000 adults, which accounts for ~ 15 % of newly diagnosed leukemias adult patients. The diagnosis based upon the genetic translocation between t(9;22)(q34;q11.2), resulting formation Philadelphia fusion chromosome, coding BCR-ABL1 oncoprotein. life-long treatment relies on tyrosine kinase inhibitors (TKIs). In nearly patients develop point mutations, leading resistance TKIs...