A5089698498- Asthma and respiratory diseases
- Autopsy Techniques and Outcomes
- COVID-19 Clinical Research Studies
- Air Quality and Health Impacts
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory Support and Mechanisms
- COVID-19 and healthcare impacts
- Long-Term Effects of COVID-19
- Climate Change and Health Impacts
- Health, Nursing, Elderly Care
- Organ Transplantation Techniques and Outcomes
- Neonatal Respiratory Health Research
- Voice and Speech Disorders
- Allergic Rhinitis and Sensitization
- SARS-CoV-2 and COVID-19 Research
- Inhalation and Respiratory Drug Delivery
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Aortic aneurysm repair treatments
- Thermal Regulation in Medicine
- Cardiovascular Disease and Adiposity
- Neuroinflammation and Neurodegeneration Mechanisms
- Global Cancer Incidence and Screening
- Cardiac Arrest and Resuscitation
- Pediatric health and respiratory diseases
- Noise Effects and Management
Universidade de São Paulo
2016-2025
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2013-2024
Fundação Faculdade de Odontologia
2023
Universidade Cidade de São Paulo
2021
Centro de Estudos da Voz
2020-2021
Universidade Estadual de Campinas (UNICAMP)
2020
Centro Universitário das Faculdades Metropolitanas Unidas
2020
Federal University of São João del-Rei
2020
Instituto do Câncer do Estado de São Paulo
2018
Instituto Federal do Sudeste de Minas Gerais
2016
Rationale: There are no reports of the systemic human pathology novel swine H1N1 influenza (S-OIV) infection.Objectives: The autopsy findings 21 Brazilian patients with confirmed S-OIV infection presented. These died in winter southern hemisphere 2009 pandemic, acute respiratory failure.Methods: Lung tissue was submitted to virologic and bacteriologic analysis real-time reverse transcriptase polymerase chain reaction electron microscopy. Expression toll-like receptor (TLR)-3, IFN-γ, tumor...
Brazil ranks high in the number of coronavirus disease 19 (COVID-19) cases and COVID-19 mortality rate. In this context, autopsies are important to confirm disease, determine associated conditions, study pathophysiology novel disease. The aim was assess systemic involvement COVID-19. order follow biosafety recommendations, we used ultrasound-guided minimally invasive autopsy (MIA-US), present results 10 initial autopsies.We MIA-US for tissue sampling lungs, liver, heart, kidneys, spleen,...
We report the case of an 11-year-old child with multisystem inflammatory syndrome in children (MIS-C) related to COVID-19 who developed cardiac failure and died after 1 day admission hospital for treatment. An otherwise healthy female African descent, patient was admitted paediatric intensive care unit (ICU) cardiovascular shock persistent fever. Her initial symptoms were fever 7 days, odynophagia, myalgia, abdominal pain. On ICU, presented respiratory distress, comprising tachypnoea...
Although increasing evidence confirms neuropsychiatric manifestations associated mainly with severe COVID-19 infection, long-term dysfunction (recently characterized as part of "long COVID-19" syndrome) has been frequently observed after mild infection. We show the spectrum cerebral impact acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ranging from alterations in mildly infected individuals (orbitofrontal cortical atrophy, neurocognitive impairment, excessive fatigue and anxiety...
Abstract The ability of the new coronavirus SARS‐CoV‐2 to spread and contaminate is one determinants COVID‐19 pandemic status. has been detected in saliva consistently, with similar sensitivity that observed nasopharyngeal swabs. We conducted ultrasound‐guided postmortem biopsies fatal cases. Samples salivary glands (SGs; parotid, submandibular, minor) were obtained. analyzed samples using RT‐qPCR, immunohistochemistry, electron microscopy, histopathological analysis identify elucidate...
COVID-19 in children is usually mild or asymptomatic, but severe and fatal paediatric cases have been described. The pathology of not known; the proposed pathogenesis for includes immune-mediated mechanisms direct effect SARS-CoV-2 on tissues. We describe autopsy findings five provide mechanistic insight into involved disease.Children adolescents who died with between March 18 August 15, 2020 were autopsied a minimally invasive method. Tissue samples from all vital organs analysed by...
Small airway disease is thought to contribute significantly functional impairment caused by asthma. Functional evidence of airway-parenchyma uncoupling in asthma, such as loss deep breath bronchodilator effect bronchoconstrictive episodes and enhanced closure, has been previously demonstrated. Elastic fibers are essential maintain adequate elastic recoil the lungs. In this study, we hypothesized that alveolar attachments could be abnormal damaged distal lungs patients with fatal For purpose,...
There is an intimate relationship between the extracellular matrix (ECM) and smooth muscle cells within airways. Few studies have comprehensively assessed composition of different ECM components its regulators airway (ASM) in asthma. With aid image analysis, fractional areas total collagen elastic fibres were quantified ASM 35 subjects with fatal asthma (FA) compared 10 nonfatal (NFA) patients 22 nonasthmatic control cases. Expression I III, fibronectin, versican, metalloproteinase (MMP)-1,...
Extracellular matrix (ECM) composition has an important role in determining airway structure. We postulated that ECM lung of chronic obstructive pulmonary disease (COPD) patients differs from observed smoking and nonsmoking subjects without airflow obstruction. determined the fractional areas elastic fibres, type-I, -III -IV collagen, versican, decorin, biglycan, lumican, fibronectin tenascin different compartments large small airways parenchyma 26 COPD patients, smokers 16 control subjects....
Background: The ability of coronavirus SARS-CoV-2 to spread is one the determinants COVID-19 pandemic status. Until June 2020, global cases surpassed 10 million. Asymptomatic patients, with no respiratory impairment, are believed be responsible for more than 80% transmission. Other viruses have been consistently detected in periodontal tissues.Objective: aim this study was investigate presence tissue.Methods: We conducted video-endoscope minimally invasive post-mortem biopsy seven fatal...
Abstract Background Pulmonary involvement in COVID-19 is characterized pathologically by diffuse alveolar damage (DAD) and thrombosis, leading to the clinical picture of Acute Respiratory Distress Syndrome. The direct action SARS-CoV-2 lung cells dysregulated immuno-coagulative pathways activated ARDS influence pulmonary severe COVID, that might be modulated disease duration individual factors. In this study we assessed proportions different pathology patterns patients along evolution...
Abstract Background Multi‐organ damage is a common feature of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection, going beyond the initially observed pneumonia. Evidence that testis also compromised growing. Objective To describe pathological findings in testes from fatal cases COVID‐19, including detection viral particles and antigens, inflammatory cell subsets. Materials methods Postmortem testicular samples were obtained by percutaneous puncture 11 deceased men...
Abstract An ideal blood biomarker for stroke should provide reliable results, enable fast diagnosis, and be readily accessible practical use. Neuron-specific enolase (NSE), an enzyme released after neuronal damage, has been studied as a marker brain injury, including cerebral infarction. However, different methodologies limited sample sizes have restricted the applicability of any potential findings. This work aims to determine whether NSE levels at Emergency Department (ED) admission...
Acute respiratory distress syndrome (ARDS) has a high mortality rate of 35-46% depending on its severity. Animal models are crucial to better understand the pathophysiology diseases, including ARDS. This study presents feasible animal model acute lung injury (ALI) using nebulized lipopolysaccharide (LPS) in non-invasive approach, focusing short and long-term effects.Mice received LPS or vehicle only (control group). Blood, BALF tissue were collected 24 hours (LPS 24h) 5 weeks 5w) after...
The regular practice of physical exercise has been associated with beneficial effects on various pulmonary conditions. We investigated the mechanisms involved in protective effect a model lipopolysaccharide (LPS)-induced acute lung injury (ALI).Mice were divided into four groups: Control (CTR), Exercise (Exe), LPS, and + LPS (Exe LPS). Exercised mice trained using low intensity daily for five weeks. Exe received 200 µg intratracheally 48 hours after last test. measured exhaled nitric oxide...
Background New strategies for collecting post-mortem tissue are necessary, particularly in areas with emerging infections. Minimally invasive autopsy (MIA) has been proposed as an alternative to conventional (CA), promising results. Previous studies using MIA addressed the cause of death adults and children developing countries. However, none these was conducted undergoing infectious disease epidemic. We have recently experienced epidemic yellow fever (YF) Brazil. Aiming provide new...
This study represents the largest compilation to date of clinical and postmortem data from decedents with coronavirus disease 2019 (COVID-19). It will augment previously published small series autopsy case reports, refine clinicopathologic considerations, improve accuracy future vital statistical reporting.To accurately reflect preexisting diseases pathologic conditions SARS-CoV-2 (severe acute respiratory syndrome 2) infection through autopsy.Comprehensive 135 evaluations COVID-19-positive...
Abstract Although increasing evidence confirms neuropsychiatric manifestations associated mainly with severe COVID-19 infection, the long-term dysfunction has been frequently observed after mild infection. Here we show spectrum of cerebral impact SARS-CoV-2 infection ranging from alterations in mildly infected individuals (orbitofrontal cortical atrophy, neurocognitive impairment, excessive fatigue and anxiety symptoms) to acute damage confirmed brain tissue samples extracted orbitofrontal...
This study tested the hypotheses that chronic allergic inflammation induces not only bronchial but also lung parenchyma remodeling, and these histologic changes are associated with concurrent in respiratory mechanics. For this purpose, airway remodeling were evaluated by quantitative analysis of collagen elastin, immunohistochemistry (smooth-muscle actin expression, eosinophil, dendritic cell densities), electron microscopy. In vivo (airway resistance, viscoelastic pressure, static...
Abstract It has been suggested that airway remodelling is responsible for the persistent obstruction and decline in lung function observed some asthmatic patients. The small airways are thought to contribute significantly this functional impairment. Proteoglycans (PGs) important components of extracellular matrix (ECM) lungs. Besides controlling biophysical properties ECM, they play roles regulation cytokines. Increased subepithelial PG deposition mild asthmatics reported. However, there no...