A5029462329- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Pharmacological Effects and Assays
- Neuropeptides and Animal Physiology
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Cardiac electrophysiology and arrhythmias
- Cardiac Imaging and Diagnostics
- Neuroscience and Neuropharmacology Research
- Phagocytosis and Immune Regulation
- Cardiac Fibrosis and Remodeling
- Transplantation: Methods and Outcomes
- Tissue Engineering and Regenerative Medicine
- Cardiac Arrhythmias and Treatments
- Eosinophilic Disorders and Syndromes
- Ion channel regulation and function
- Cardiovascular Health and Risk Factors
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- Adipose Tissue and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Malaria Research and Control
Northwestern University
2013-2025
Northwestern Memorial Hospital
2012-2023
Northwestern Medicine
2021
Columbia University
2013
CECOLFES
2012
Brigham and Women's Hospital
2012
Harvard University
2012
Northwestern University
2007
Boston College
2004
Vanderbilt University
2003
A novel alpha 1-adrenergic receptor subtype has been cloned from a bovine brain cDNA library. The deduced amino acid sequence is that of 466-residue polypeptide. structure similar to the other adrenergic receptors as well larger family G protein-coupled have presumed seven-membrane-spanning domain topography. greatest identity this protein with previously hamster 1B-adrenergic being approximately 72% within membrane-spanning domains. Localization on different human chromosomes provides...
Pharmacological and molecular cloning studies have demonstrated heterogeneity of alpha 1-adrenergic receptors. We now cloned two receptors from a rat cerebral cortex cDNA library, using the hamster 1B-adrenergic receptor as probe. The deduced amino acid sequence clone RA42 encodes protein 560 acids whose putative topology is similar to that family G-protein-coupled primary structure though most closely resembles an receptor, having approximately 73% identity in transmembrane domains with...
Controlling new blood vessel formation is of interest in regenerative medicine and cancer treatment. Heparin, a biopolymer that binds to angiogenic growth factors, was used nucleate the self-assembly nanostructures from designed peptide amphiphile molecules. This process yields rigid nanofibers display heparin chains orient proteins for cell signaling. In vivo, stimulated extensive using nanogram amounts growth-factor by themselves did not induce any detectable angiogenesis.
DDT1 MF-2 cells, which are derived from hamster vas deferens smooth muscle, contain alpha 1-adrenergic receptors (54,800 +/- 2700 sites per cell) that coupled to stimulation of inositol phospholipid metabolism. Incubation these cells with tumor-promoting phorbol esters, stimulate calcium- and phospholipid-dependent protein kinase, leads a marked attenuation the ability 1-receptor agonists such as norepinephrine turnover phospholipids. This was measured by determining 32P content...
Rationale: Efficient clearance of apoptotic cells (efferocytosis) is a prerequisite for inflammation resolution and tissue repair. After myocardial infarction, phagocytes are recruited to the heart promote dying cardiomyocytes. The molecular mechanisms efferocytosis cardiomyocytes in myocardium unknown. injured provides unique model examine relationships between subsequent resolution, remodeling, organ function. Objective: We set out identify cardiomyocyte engulfment by and, first time,...
Some patients with severe COVID-19 develop end-stage pulmonary fibrosis for which lung transplantation may be the only treatment.
No AccessJournal of Urology1 Aug 1993Identification, Quantification, and Localization mRNA for Three Distinct Alpha1 Adrenergic Receptor Subtypes in Human Prostate David T. Price, Debra A. Schwinn, Jon W. Lomasney, Lee F. Allen, Marc G. Caron, Robert J. Lefkowitz PriceDavid Price More articles by this author , SchwinnDebra Schwinn LomasneyJon Lomasney AllenLee Allen CaronMarc Caron LefkowitzRobert View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)35544-1AboutPDF ToolsAdd to...
Pharmacologic, biochemical, and genetic analyses have demonstrated the existence of multiple alpha 2-adrenergic receptor (alpha 2AR) subtypes. We cloned a human 2AR by using polymerase chain reaction with oligonucleotide primers homologous to conserved regions previously 2ARs, genes for which are located on chromosomes 4 (C4) 10 (C10). The deduced amino acid sequence encodes protein 450 acids whose putative topology is similar that family guanine nucleotide-binding protein-coupled receptors,...
Three families of phospholipase C (PI-PLCβ, γ, and δ) are known to catalyze the hydrolysis polyphosphoinositides such as phosphatidylinositol 4,5-bisphosphate (PIP<sub>2</sub>) generate second messengers inositol 1,4,5 trisphosphate diacylglycerol, leading a cascade intracellular responses that result in cell growth, differentiation, gene expression. Here we describe founding member novel, structurally distinct fourth family PI-PLC. PLCε not only contains conserved catalytic (X Y) regulatory...
On the basis of extensive radioligand data and more limited functional data, three pharmacological subtypes alpha 2-adrenergic receptors have been identified. More recently, human genes or cDNAs for identified by molecular cloning. The relationship, however, among pharmacologically defined those cloning has not clear. In order to resolve this issue, we compared characteristics expressed in COS-7 cells with their respective prototypic tissue cell line. affinities (Ki values) 12...
BACKGROUNDSurvivors of pneumonia, including SARS-CoV-2 are at increased risk for cognitive dysfunction and dementia. In rodent models, following pneumonia has been linked to the systemic release lung-derived pro-inflammatory cytokines. Microglia poised respond inflammatory signals from circulation, their impairment in murine models dementia humans.METHODSWe measured levels 55 cytokines chemokines bronchoalveolar lavage fluid plasma 341 patients with respiratory failure 13 healthy controls,...
Erythrocytic mechanisms involved in malarial infection are poorly understood. We have found that signaling via the erythrocyte β2-adrenergic receptor and heterotrimeric guanine nucleotide–binding protein (Gαs) regulated entry of human malaria parasite Plasmodium falciparum . Agonists stimulate cyclic adenosine 3′,5′-monophosphate production led to an increase could be blocked by specific antagonists. Moreover, peptides designed inhibit Gαs function reduced parasitemia P. cultures vitro,...
Based on biochemical and ligand binding studies in various tissues species, evidence for several alpha 2-adrenergic receptor subtypes has accumulated. The current classification (alpha 2A, 2B, 2C) is based exclusively pharmacological criteria. molecular cloning of three distinct genes human receptors confirmed the existence multiple subtypes. According to their localization different chromosomes, were termed 2-C10, 2-C4, 2-C2. relationship, however, between pharmacologically characterized...
Background— Atrial fibrillation (AF) is commonly associated with congestive heart failure (CHF). The autonomic nervous system involved in the pathogenesis of both AF and CHF. We examined role remodeling contributing to substrate Methods Results— Electrophysiological mapping was performed pulmonary veins left atrium 38 rapid ventricular–paced dogs (CHF group) 39 control under following conditions: vagal stimulation, isoproterenol infusion, β-adrenergic blockade, acetylcholinesterase (AChE)...
The mechanism for stem cell-mediated improvement following acute myocardial infarction has been actively debated. We support hypotheses that the cell effect is primarily paracrine factor-linked. used a heparin-presenting injectable nanofibre network to bind and deliver factors derived from hypoxic conditioned media mimic this effect. Our self-assembling peptide nanofibres presenting heparin were capable of binding medium phase. When these factor-loaded materials injected into heart coronary...
This study represents the largest compilation to date of clinical and postmortem data from decedents with coronavirus disease 2019 (COVID-19). It will augment previously published small series autopsy case reports, refine clinicopathologic considerations, improve accuracy future vital statistical reporting.To accurately reflect preexisting diseases pathologic conditions SARS-CoV-2 (severe acute respiratory syndrome 2) infection through autopsy.Comprehensive 135 evaluations COVID-19-positive...
The pleckstrin homology (PH) domain is a newly recognized protein module believed to play an important role in signal transduction. While the tertiary structures of several PH domains have been determined, some co-complexed with ligands, function this remains elusive. In report, located N terminus human phospholipase C-delta1 (PLCdelta1) was found regulate enzyme activity. hydrolysis phosphatidylinositol (PI) stimulated by 4,5-bisphosphate (PIP2) dose-dependent manner EC50 = 1 microM (0.3...
alpha 1-Adrenergic receptors from a cultured smooth muscle cell line (DDT1 MF-2) have been solubilized with digitonin and purified to apparent homogeneity by sequential chromatography on biospecific affinity support (Sepharose-A55453 (4-amino-6,7-dimethoxy-2-[4-[5-(4-amino-3-phenyl) pentanoyl]-1-piperazinyl]-quinazoline), an 1 receptor-selective antagonist), wheat germ agglutinin-agarose gel, high performance steric exclusion liquid column. Sodium dodecyl sulfate-polyacrylamide gel...