A5101629302- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Weed Control and Herbicide Applications
- Multiple Sclerosis Research Studies
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- Systemic Lupus Erythematosus Research
- Agronomic Practices and Intercropping Systems
- Cytokine Signaling Pathways and Interactions
- Pancreatic function and diabetes
- Pesticide and Herbicide Environmental Studies
- interferon and immune responses
- Crop Yield and Soil Fertility
- Animal Virus Infections Studies
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Neurogenesis and neuroplasticity mechanisms
- Allelopathy and phytotoxic interactions
- Agriculture, Plant Science, Crop Management
- RNA regulation and disease
- Herpesvirus Infections and Treatments
Northwestern University
2016-2025
Le Bonheur Children's Hospital
2024
University of Tennessee Health Science Center
2022-2024
Rutgers Sexual and Reproductive Health and Rights
2023
Oregon Health & Science University
2023
Lehigh Valley Hospital-Pocono
2023
Lehigh Valley Health Network
2023
Virginia Commonwealth University Medical Center
2023
University of Arizona
2023
Medical College of Wisconsin
2023
Abstract Microglia are the resident macrophage-like population in CNS. remain quiescent until injury or infection activates cells to perform effector inflammatory and APC functions. Our previous studies have shown that microglia infected with a neurotropic strain of Theiler’s murine encephalomyelitis virus secreted innate immune cytokines up-regulated costimulatory molecules MHC class II, enabling present viral myelin Ags CD4+ T cells. Recently, TLRs been recognize pathogen-associated...
Autoreactive CD4(+) T cells exist in normal individuals and retain the capacity to initiate autoimmune disease. The current study investigates role of CD4(+)CD25(+) T-regulatory (T(R)) during disease using cell-dependent myelin oligodendrocyte glycoprotein (MOG)-specific experimental encephalomyelitis model multiple sclerosis. In vitro, T(R) effectively inhibited both proliferation cytokine production by MOG(35-55)-specific Th1 cells. vivo, adoptive transfer conferred significant protection...
The role of epitope spreading in the pathology relapsing-remitting experimental autoimmune encephalomyelitis (R-EAE) was examined. Using peripherally induced immunologic tolerance as a probe to analyze neuropathologic T cell repertoire, we show that majority immunopathologic reactivity during acute phase R-EAE SJL/J mice by active immunization with intact proteolipid (PLP) molecule is directed at PLP139-151 and responses secondary encephalitogenic PLP epitopes may contribute later relapsing...
Cell-based therapies are attractive approaches to promote myelin repair. Recent studies demonstrated a reduction in disease burden mice with experimental allergic encephalomyelitis (EAE) treated mouse mesenchymal stem cells (MSCs). Here, we human bone marrow-derived MSCs (BM-hMSCs) functional recovery both chronic and relapsing-remitting models of EAE, traced their migration into the injured CNS assayed ability modulate progression host immune response. Injected BM-hMSCs accumulated CNS,...
Experimental autoimmune encephalomyelitis (EAE) is a CD4+ T cell-mediated, inflammatory demyelinating disease of the central nervous system (CNS) that serves as model for human disease, multiple sclerosis. A critical event in pathogenesis EAE entry both Ag-specific lymphocytes and Ag-nonspecific mononuclear cells into CNS. In present report we investigated role two C-C chemokines (macrophage protein-1 alpha (MIP-1 alpha) monocyte chemotactic protein-1) C-x-C chemokine (MIP-2) EAE. Production...
The interplay between innate and adaptive immunity is important in multiple sclerosis (MS). inflammasome complex, which activates caspase-1 to process pro-IL-1beta pro-IL-18, rapidly emerging as a pivotal regulator of immunity, with nucleotide-binding domain, leucine-rich repeat containing protein family, pyrin domain 3 (NLRP3) (cryopyrin or NALP3) prominent player. Although the role NLRP3 host response pathogen associated molecular patterns danger well documented, its autoimmune diseases...
This unit details the materials and methods required for both active induction adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in SJL mouse strain using intact proteins or peptides from two major myelin proteins: proteolipid protein (PLP) basic (MBP). Detailed purification PLP MBP are also described. Modifications specified protocols may be necessary efficient EAE other strains.
CTLA-4, a CD28 homologue expressed on activated T cells, binds with high affinity to the ligands, B7-1 (CD80) and B7-2 (CD86). This study was designed examine role of CTLA-4 in regulating autoimmune disease. Murine relapsing-remitting experimental encephalomyelitis (R-EAE) is demyelinating disease mediated by PLP139-151-specific CD4+ cells SJL/J mice. Anti-CTLA-4 mAbs (or their F(ab) fragments) enhanced vitro proliferation pro-inflammatory cytokine production PLP139-151-primed lymph node...
Abstract The immunologic privilege of the central nervous system (CNS) makes it crucial that CNS resident cells be capable responding rapidly to infection. Astrocytes have been reported express Toll‐like receptors (TLRs), hallmark pattern recognition innate immune system, and respond their ligation with cytokine production. also cytokines adaptive induction antigen presentation functions. Here we compared ability TLR stimuli interferon‐γ (IFN‐γ) tumor necrosis factor‐α (TNF‐α) induce a...
Negatively charged immune-modifying microparticles bind to the scavenger receptor MARCO on inflammatory monocytes, resulting in their apoptosis and reduced damage a range of diseases.
Abstract CD4+CD25+ T regulatory (TR) cells are an important component of the adaptive immune system that limit autoreactive cell responses in various models autoimmunity. This knowledge was generated by previous studies from our lab and others using TR supplementation depletion. Contrary to dogma, we report here injection anti-CD25 mAb results functional inactivation, not depletion, cells, resulting exacerbated autoimmune disease. Supporting this, mice receiving treatment display...
Rationale: Efficient clearance of apoptotic cells (efferocytosis) is a prerequisite for inflammation resolution and tissue repair. After myocardial infarction, phagocytes are recruited to the heart promote dying cardiomyocytes. The molecular mechanisms efferocytosis cardiomyocytes in myocardium unknown. injured provides unique model examine relationships between subsequent resolution, remodeling, organ function. Objective: We set out identify cardiomyocyte engulfment by and, first time,...
Targeted immune tolerance is a coveted therapy for the treatment of variety autoimmune diseases, as current options often involve nonspecific immunosuppression. Intravenous (iv) infusion apoptotic syngeneic splenocytes linked with peptide or protein autoantigens using ethylene carbodiimide (ECDI) has been demonstrated to be an effective method inducing peripheral, antigen-specific disease. Here, we show ability biodegradable poly(lactic-co-glycolic acid) (PLG) nanoparticles function safe,...
Metastatic cancers produce exosomes that condition pre-metastatic niches in remote microenvironments to favor metastasis. In contrast, here we show from poorly metastatic melanoma cells can potently inhibit metastasis the lung. These "non-metastatic" stimulate an innate immune response through expansion of Ly6C
Antigen-coupled cells result in antigen-specific tolerization for treatment of multiple sclerosis.