A5045311567- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Nitric Oxide and Endothelin Effects
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Lipid Membrane Structure and Behavior
- Phytoestrogen effects and research
- Adenosine and Purinergic Signaling
- Vestibular and auditory disorders
- Hearing, Cochlea, Tinnitus, Genetics
- Ion Channels and Receptors
- Mass Spectrometry Techniques and Applications
- Electrolyte and hormonal disorders
- Estrogen and related hormone effects
- Eicosanoids and Hypertension Pharmacology
- Electrochemical Analysis and Applications
- Antimicrobial Peptides and Activities
- Diet, Metabolism, and Disease
- Neonatal Health and Biochemistry
- Birth, Development, and Health
- Epilepsy research and treatment
- Cardiac Ischemia and Reperfusion
- Prenatal Substance Exposure Effects
- Cell Adhesion Molecules Research
Universidad Nacional de La Plata
2011-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2024
Instituto de Física La Plata
2019-2023
Centro Científico Tecnológico - La Plata
2021-2023
Hospital de Niños Superiora Sor María Ludovica
2006
Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación
2001
University of Pavia
1993-1995
University of Turin
1992
The purpose of this investigation is to contribute the development new anticonvulsant drugs treat patients with refractory epilepsy. We applied a virtual screening protocol that involved search into molecular databases compounds and known find small molecules interact open conformation Nav1.2 pore. As 3D structure human not available, we first assembled models target, in closed conformations. After screening, resulting candidates were submitted second filter, better chances being effective...
Cannabidiol (CBD), one of the main Cannabis sativa bioactive compounds, is utilized in treatment major epileptic syndromes. Its efficacy can be attributed to a multimodal mechanism action that includes, as potential targets, several types ion channels. In brain, CBD reduces firing frequency rat hippocampal neurons, partly prolonging duration potentials, suggesting blockade voltage-operated K+ We postulate this effect might involve inhibition large-conductance voltage- and Ca2+-operated...
The mechanism whereby extracellular Ca(2+) exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional Ca(2+)-sensing receptor (CaSR) using variety techniques. CaSR mRNA was detected RT-PCR, and protein identified by immunocytochemical analysis. order to assess functionality receptor, CMEC were loaded with Ca(2+)-sensitive fluorochrome, Fura-2/AM. A number agonists, such as...
We studied the kinetics of extracellular ATP (ATPe) in Escherichia coli and their outer membrane vesicles (OMVs) stimulated with amphipatic peptides melittin (MEL) mastoparan 7 (MST7). Real-time luminometry was used to measure ATPe kinetics, release, ATPase activity. The latter also determined by following [32P]Pi released from [γ-32P]ATP. E. alone, co-incubated Caco-2 cells, or rat jejunum segments. In coli, addition [γ-32P]ATP led uptake subsequent hydrolysis ATPe. Exposure caused an acute...
Epilepsy is a neurological disorder characterized by recurrent seizures that arise from abnormal electrical activity in the brain. Voltage-gated sodium channels (NaVs), responsible for initiation and propagation of action potentials neurons, play critical role pathogenesis epilepsy. This study sought to discover potential anticonvulsant compounds interact with NaVs, specifically, brain subtype hNaV1.2. A ligand-based QSAR model docking were constructed, validated, applied parallel virtual...
The electrophysiological and pharmacological properties of Ca2+ current (ICa) were determined by the whole-cell configuration patch-clamp technique in smooth muscle cells from human umbilical artery. Using 5 mM extracellular Ca2+, depolarizing step pulses -60 to 50 mV a holding membrane potential -80 evoked an ICa which activated at potentials more positive than -50 exhibited maximum density range 10-20 mV. Steady-state inactivation protocols using Vtest 10 gave voltage one-half slope factor...
Bupivacaine is a local anesthetic compound belonging to the amino amide group. Its effect commonly related its inhibitory on voltage-gated sodium channels. However, several studies have shown that this drug can also inhibit voltage-operated K+ channels by different blocking mechanism. This could explain observed contractile effects of bupivacaine blood vessels. Up now, there were no previous reports in literature about large conductance voltage- and Ca2+-activated (BKCa). Using patch-clamp...
Vestibular type I and II hair cells their afferent fibres send information to the brain regarding position movement of head. The characteristic feature is expression a low-voltage-activated outward rectifying K+ current, IK,L , whose biophysical properties molecular identity are still largely unknown. In vitro, nerve calyx surrounding causes unstable intercellular concentrations, altering . We found that in absence calyx, exhibited unique activation properties, which were faithfully...
Abstract The influence of the conditions for isolation soy protein on content genistein and its conjugated forms was studied. major components series isolated from soybean flour were malonyl genistin (54.3%), (36.9%), equal amounts (4.4%) acetyl genistin. A modification in conjugation profile between pH 4.5 8.0 above 10 attributed to action β ‐glucosidase saponification reaction, respectively. decrease total insoluble residue isolate (SPI) with increasing extraction detected, while whey, not...
Background Intestinal transplantation (ITx) faces many challenges due to the complexity of surgery and multiple immunological reactions that lead necessity rigorous follow-up for early detection acute cellular rejection (ACR). Our aim was determine kinetics ACR using an experimental ITx model, with emphasis in characterization process different approaches, including use functional assays absorptive barrier function. Methods rats conducting serial sampling performed. Clinical monitoring,...
Propylparaben, a commonly used antimicrobial preservative, has been reported as an anticonvulsant agent targeting neuronal Na+ channels (NaV). However, the specific features of NaV channel inhibition by this have so far not extensively studied. Moreover, it is still unclear if shares pharmacological activity with other parabens. Here, we fully characterized mechanism action inhibitory effect that propylparaben and benzylparaben induce on human 1.2 isoform (hNaV1.2). We established first...
Abstract Metabolic reprogramming of cancer cells results in a high production acidic substances that must be extruded to maintain tumor‐cell viability. The voltage‐gated proton channel (Hv1) mediates highly selective effluxes hydronium‐ion (H + ) prevent deleterious cytoplasmic acidification. In the work described here, we demonstrated for first time amino‐terminal–truncated isoform Hv1 is more expressed tumorigenic breast‐cancer‐cell lines than nontumorigenic breast cells. With respect...