BACKGROUND AND PURPOSE β‐Adrenoceptors are expressed in human and experimental animal breast cancer cells. However, the effect of agonists antagonists reported on cell proliferation tumour growth was paradoxical, precluding their utilization as possible adjuvant therapy, mainly cases refractory tumours. EXPERIMENTAL APPROACH β‐Adrenoceptor expression analysed by immunofluorescence RT‐PCR. Cell assessed [ 3 H]‐thymidine incorporation, measuring with a calliper ERK 1/2 phosphorylation Western...

Breast cancer, the most common cancer in women countries, is a highly stressful disease. Catecholamines released during stress bind to adrenoceptors and we have recently described alpha(2)-adrenoceptors human breast cell lines, linked enhanced proliferation. The purpose was assess vivo effects of compounds acting on reliable model cancer.The expression confirmed by immunocytochemistry, immunofluorescence reverse transcription-PCR mouse mammary tumour line MC4-L5. Proliferation assessed...

Quercetin (QUE) is a phytoestrogen with known antitumor properties; however, its hydrophobic nature and low bioavailability limit efficacy as an anticancer drug. To address this, we explored loading QUE onto non-toxic nanocarrier. This study focused on the biological activity of magnetic iron oxide nanoparticles coated polyethylene glycol (MAG@PEG) loaded (MAG@PEG@QUE) in MCF-7 cells. The MAG@PEG nanosystem was synthesized using hydrothermal method, incorporated by adding alcoholic solution...

We have previously described enhanced human breast cancer cell proliferation and mouse mammary tumor growth induced by α(2)-adrenoceptor (α(2)-AR) expression in epithelial cells. The aim of the present work was to assess if stromal fibroblasts can contribute this effect. α(2)-AR assessed immunocytochemistry immunohistochemistry, [(3)H]-Thymidine incorporation measuring with caliper. All tested expressed at least two subtypes α(2)-adrenergic agonists fibroblast proliferation. In vivo, agonist...

// Sabrina Copsel 1,2 , Ariana Bruzzone 1 Maria May Julien Beyrath 3 Victoria Wargon Jeannette Cany 4 Frans G.M. Russel Carina Shayo and Carlos Davio 2 Instituto de Biología y Medicina Experimental-CONICET, Buenos Aires, Argentina Laboratorio Farmacología Receptores, Facultad Farmacia Bioquímica, Universidad Department of Pharmacology Toxicology, Radboud University Medical Center, Nijmegen, The Netherlands Laboratory Medicine, Correspondence: Copsel, email: Keywords :...

// Lucía Gargiulo 1, * , Sabrina Copsel 2, Ezequiel M. Rivero 1 Céline Galés 3 Jean-Michel Sénard Isabel A. Lüthy Carlos Davio Ariana Bruzzone Instituto de Biología y Medicina Experimental-CONICET, Vuelta Obligado 2490, C1428ADN, CABA, Argentina 2 Laboratorio Farmacología Receptores, Departamento Farmacología, Facultad Farmacia Bioquímica, Universidad Buenos Aires, Junin 956 (1113) Institut des Maladies Métaboliques et Cardiovasculaires, National la Santé Recherche Médicale, U1048,...

The role of β-adrenergic receptors (β-ARs) remains controversial in normal and tumor breast. Herein we explore the cAMP signaling involved β -AR-dependent control proliferation adhesion nontumor human breast cell line MCF-10A. Low concentrations a β-agonist, isoproterenol (ISO), promote (87.5% cells remaining adherent to plastic dishes following specific detachment vs. 35.0% control, P<0.001), while increasing further engages an additional 36% inhibition Erk1/2 phosphorylation...

The cholinergic α7 nicotinic receptor gene, CHRNA7, encodes a subunit that forms the homopentameric receptor, involved in learning and memory. In humans, exons 5–10 CHRNA7 are duplicated fused to FAM7A genetic element, giving rise hybrid gene CHRFAM7A. Its product, dupα7, is truncated lacking part of N-terminal extracellular ligand-binding domain associated with neurological disorders, including schizophrenia, immunomodulation. We combined dupα7 expression on mammalian cells patch clamp...

Aims Breast cancer is the most frequently diagnosed and leading cause of death among women worldwide. It was classified within molecular intrinsic subtypes: luminal A, B, human epidermal growth factor receptor 2‐enriched basal‐like. Epinephrine norepinephrine, released during stress, bind to adrenoceptors. α 2 ‐adrenoceptors are encoded by ADRA2A , ADRA2B ADRA2C genes β ADRB2 . Methods We compiled several publicly available Affymetrix gene expression datasets, obtaining a large cohort 1924...

Breast cancer is the most diagnosed and major cause of death in women worldwide. Metastasis main these deaths. The metastatic cascade involves multiple steps it has been described that adrenergic receptors can modulate this process at levels. However, β -adrenergic action breast controversial. We have previously shown β-adrenergic agonists inhibit cell proliferation tumor growth numerous models.The purpose present investigation was to evaluate effect parameters related progression...

The imidazoline nucleus linked in position 2 via an oxyethylene bridge to a phenyl ring carrying ortho substituent of moderate steric bulk provided α2-adrenergic (AR) ligands endowed with significant α2C-agonism/α2A-antagonism. Similar behavior was displayed by cirazoline (12). For their positive morphine analgesia modulation (due α2C-AR stimulation) and sedation overcoming α2A-AR antagonism), 8 11 might be useful as adjuvant agents the management pain morphine.

Abstract Breast cancer is the most frequent in women. However, vivo hormone receptor positive and metastatic models are scarce. The aim of present manuscript was to assess if novel steroid human cell lines IBH‐4, IBH‐6, IBH‐7 developed our laboratory from primary infiltrant ductal carcinomas good study breast cancer. Cell or tumors were inoculated nude mice presence absence supplementation. Growth analyzed by ANOVA followed Tukey–Kramer's test. Steroid expression assessed...

The β-blocker propranolol (PROP) has been proposed as a repurposed treatment for breast cancer. similarity of action between β-agonists and antagonists found on cells encouraged us to compare PROP isoproterenol (ISO, agonist) signaling pathways human cell line. Cell proliferation was measured by counting DNA-synthesis. adhesion the that remained adhered plastic after different treatments. Changes in actin cytoskeleton were observed fluorescence staining Western Blot. ISO caused diminution an...

Breast cancer is the most frequent malignancy in women majority of western countries. Both acute and chronic stress cause release epinephrine norepinephrine. These natural cateholamines bind to nine different adrenoceptors. epithelial cells express α2 β2-adrenoceptors. Catecholamine binding α2-adrenoceptors associated with increased cell proliferation. On contrary, catecholamine β2-adrenoceptors result diminished Therefore, relative concentration these receptors tumor will enhance or...

Abstract Yolk platelets, the principal components of amphibian oocytes, have been generally considered as material reservoirs. Their biochemical composition and function during oogenesis early development not fully elucidated. The aim this study was to carry out a lipidic characterization yolk platelets from full‐grown Bufo arenarum oocytes. Ovarian oocytes were manually obtained subcellular fraction isolated by centrifugation at low velocity. Lipids separated thin‐layer chromatography. For...

The content and composition of phospholipids triacylglycerols (TAGs) in Bufo arenarum oocytes stages III IV their oogenesis were studied. total amount stage is 0.5-fold higher than oocytes. In both cases, the main are phosphatidylcholine (PC) phosphatidylethanolamine (PE). A striking observation concerns high level diphosphatidylglycerol (DPG) oocytes, which could be indicative a relatively larger mitochondrial population with respect to other oogenetic stages. net increase sphingomyelin was...