A5075042199- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Bacteriophages and microbial interactions
- Glioma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- PARP inhibition in cancer therapy
- Epigenetics and DNA Methylation
- Quantum Dots Synthesis And Properties
- Molecular Junctions and Nanostructures
- Acute Myeloid Leukemia Research
- Computational Drug Discovery Methods
- Cell death mechanisms and regulation
- Green IT and Sustainability
- Mycotoxins in Agriculture and Food
- Advanced Biosensing Techniques and Applications
- Nanoplatforms for cancer theranostics
- Chemical Synthesis and Analysis
- Carcinogens and Genotoxicity Assessment
- Folate and B Vitamins Research
- Gold and Silver Nanoparticles Synthesis and Applications
ETH Zurich
2017-2025
Department of Medical Sciences
2025
McGill University
2019-2025
Carleton University
2010-2016
Stanford University
2013-2016
Library and Archives Canada
2012
Aptamers are single-stranded oligonucleotides that bind to targets with high affinity and selectivity. Their use as molecular recognition elements has emerged a viable approach for biosensing, diagnostics, therapeutics. Despite this potential, relatively few aptamers exist small molecules. Small molecules important investigation due their diverse biological functions well clinical commercial uses. Novel, effective probes these compounds therefore of great interest. This paper will highlight...
Nucleic acid aptamers are versatile molecular recognition agents that bind to their targets with high selectivity and affinity. The past few years have seen a dramatic increase in aptamer development interest for diagnostic therapeutic applications. As the applications expand, need more standardized, stringent, informative characterization validation methodology increases. Here we performed comprehensive analysis of panel conventional affinity binding assays using suite small molecule target...
Fumonisins are mycotoxins produced by Fusarium verticillioides and F. proliferatum, fungi that ubiquitous in corn (maize). Insect damage some other environmental conditions result the accumulation of fumonisins corn-based products worldwide. Current methods fumonisin detection rely on use immunoaffinity columns high-performance liquid chromatography (HPLC). The aptamers offers a good alternative to antibodies cleanup due lower costs improved stability. Aptamers single-stranded...
Nucleic acid aptamers are emerging as useful molecular recognition tools for food safety monitoring. However, practical and technical challenges limit the number diversity of available aptamer probes that can be incorporated into novel sensing schemes. This work describes selection DNA bind to important contaminant ochratoxin A (OTA). Following 15 rounds in vitro selection, sequences were analyzed OTA binding. Two isolated demonstrated high affinity binding selectivity this mycotoxin...
Nucleic acid aptamers function as versatile sensing and targeting agents for analytical, diagnostic, therapeutic, gene-regulatory applications, but their limited characterization functional validation have hindered broader implementation. We report the development of a surface plasmon resonance-based platform rapid kinetic equilibrium binding properties to small molecules. Our system is label-free scalable enables analysis different aptamer-target pairs conditions with same platform. This...
Single-nucleotide-resolution sequencing of DNA damage is required to decipher the complex causal link between identity and location adducts their biological impact. However, low abundance inability specifically amplify hinders single-nucleotide mapping within whole genomes. Despite high relevance guanine oxidation seminal recent advances in bulky adducts, single-nucleotide-resolution genome oxidative not yet realized. We coupled specificity repair enzymes with efficiency a click ligation...
Significance Poly(ADP-ribose) polymerase-1 (PARP-1) is a DNA repair protein and part of the genome maintenance network. On other hand, PARP-1 involved in pathophysiological processes such as inflammation. Chronic inflammation has emerged key event carcinogenesis, including formation colorectal cancer (CRC). Our data reveal that abundantly expressed human CRC, correlating with disease progression. Using transgenic mouse models, we show fosters inflammation-driven tumor growth stimulates...
Over the past several decades, rapid developments in both molecular and information technology have collectively increased our ability to understand recognition. One emerging area of interest recognition research includes isolation aptamers. Aptamers are single-stranded nucleic acid or amino polymers that recognize bind targets with high affinity selectivity. While has focused on collecting aptamers their interactions, most regarding experimental methods remains unstructured textual format...
It is well known that using random RNA/DNA sequences for SELEX experiments will generally yield low-complexity structures. Early experimental results suggest having a structurally diverse library, which, instance, includes high-order junctions, may prove useful in finding new functional motifs. Here, we develop two computational methods to generate exhibit higher structural complexity and can be used increase the overall diversity of initial pools vitro selection experiments. Random...
Abstract Synthetic RNA-based genetic devices dynamically control a wide range of gene-regulatory processes across diverse cell types. However, the limited throughput quantitative assays in mammalian cells has hindered fast iteration and interrogation sequence space needed to identify new RNA devices. Here we report developing quantitative, rapid high-throughput cell-based RNA-Seq assay efficiently engineer We ribozyme-based that respond theophylline, hypoxanthine, cyclic-di-GMP, folinic acid...
Chemical modifications to DNA bases, including adducts arising from reactions with electrophilic chemicals, are well-known impact cell growth, miscode during replication, and influence disease etiology. However, knowledge of how genomic sequences structures the accumulation alkylated bases is not broadly characterized high resolution, nor have these patterns been linked overall quantities modified in genome. For benzo(a) pyrene (BaP), a ubiquitous environmental carcinogen, we developed...
Measuring interactions between macromolecules is essential for elucidating their dynamics in solution and critical the design study of potential therapeutics. Fluorescence anisotropy has been a powerful widely used tool studying binding interactions. When fluorescent partner excited with polarized light while bound to its cognate ligand, emitted partially polarized. This process dependent on concentration molecules, permitting determination affinity. Here, we outline highly modular...
Abstract Temozolomide kills cancer cells by forming O6-methylguanine (O6-MeG), which leads to cell cycle arrest and apoptosis. However, O6-MeG repair O6-methylguanine-DNA methyltransferase (MGMT) contributes drug resistance. Characterizing genomic profiles of could elucidate how accumulation is influenced repair, but there are no methods map locations O6-MeG. Here, we developed an immunoprecipitation- polymerase-stalling-based method, termed O6-MeG-seq, locate across the whole genome at...
Fluorescent probe-based quantitative polymerase chain reaction (qPCR) is essential for DNA/RNA quantification widely used in research and clinical diagnostics. The performance of fluorogenic probes depends heavily on their design, particularly the identities fluorophore quencher moieties, linkers to attach them oligonucleotides. Here we report a highly modular, three-way branched glycerol 'X' linker TaqMan® type oligonucleotide multiplexed, reverse transcription qPCR (RT-qPCR). flexible...
Aptamers are versatile sensing elements for the construction of biosensors. A common approach signal generation in "aptasensors" involves displacement short complementary "probes" resulting from conformational changes upon aptamer-target binding. However, designing strands that rapidly and completely displace when target binds is nontrivial. Typically, probes discovered through a lengthy process screening several potential sequences. Here, we explored properties governing probe efficiency...
Magnetic separation has gained new popularity as a versatile partitioning method with the recent growth in nanotechnology and related biotechnology applications. In this study, iron oxide magnetic nanoparticles were synthesized via solvothermal methods directly coated gold to form core-shell gold-coated (Fe3O4-AuNPs). High-resolution transmission electron microscopy Energy dispersive X-ray spectroscopy results suggests that temperature reaction time play an important role formation of small,...
Cadmium-containing quantum dot (QD) nanoparticles are integrated into electronic displays because of their ability to efficiently convert colors. There conflicting accounts as whether these particles present a hazard the environment, they have been studied either (1) embedded QDs in display screen films or (2) model with small, hydrophilic ligands. Both approaches limitations that we addressed by synthesizing featuring core–shell structure and thick polymer coating commercial devices probe...
Abstract Differentiation therapy has proven to be a success story for patients with acute promyelocytic leukemia. However, the remaining subtypes of myeloid leukemia (AML) are treated cytotoxic chemotherapies that have limited efficacy and high likelihood resistance. As differentiation arrest is hallmark AML, there increased interest in developing differentiation-inducing agents enhance disease-free survival. Here, we provide comprehensive review current reports future avenues nucleic acid...