A5026431454- Microbial Community Ecology and Physiology
- Atmospheric Ozone and Climate
- Fullerene Chemistry and Applications
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Atmospheric chemistry and aerosols
- Legume Nitrogen Fixing Symbiosis
- Cardiac Ischemia and Reperfusion
- Carcinogens and Genotoxicity Assessment
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Organic Chemistry Cycloaddition Reactions
- Cardiac Arrest and Resuscitation
- Genomics and Phylogenetic Studies
- Wastewater Treatment and Nitrogen Removal
- Atmospheric aerosols and clouds
- Protist diversity and phylogeny
- Bacterial Genetics and Biotechnology
- Pulsars and Gravitational Waves Research
- Carbon Nanotubes in Composites
- Genomics, phytochemicals, and oxidative stress
- Biochemical Acid Research Studies
- PARP inhibition in cancer therapy
- Biochemical and Molecular Research
- Respiratory Support and Mechanisms
University of Freiburg
2023-2024
Nema Research (United States)
2022
Karolinska Institutet
2022
Temple University
2022
Fondazione Roma
2022
Uppsala University
2022
Ruhr University Bochum
2014-2021
Johannes Gutenberg University Mainz
2015-2018
University Medical Center of the Johannes Gutenberg University Mainz
2015-2018
The University of Texas at Austin
2015
This paper presents the measurements of OH and HO 2 radical concentrations as well photolysis frequencies different molecules during Berliner Ozone (BERLIOZ) field experiment in July/August 1998 at rural site Pabstthum about 50 km NW Berlin. Radical were measured using laser‐induced fluorescence (LIF) spectroscopy, while filter radiometers a scanning spectroradiometer used to obtain frequencies. The data set covers time period from 20 July 6 August consists more than 6000 simultaneous with...
Significance Poly(ADP-ribose) polymerase-1 (PARP-1) is a DNA repair protein and part of the genome maintenance network. On other hand, PARP-1 involved in pathophysiological processes such as inflammation. Chronic inflammation has emerged key event carcinogenesis, including formation colorectal cancer (CRC). Our data reveal that abundantly expressed human CRC, correlating with disease progression. Using transgenic mouse models, we show fosters inflammation-driven tumor growth stimulates...
Abstract When the supply of inorganic carbon is limiting, photosynthetic cyanobacteria excrete nitrite, a toxic intermediate in ammonia assimilation pathway from nitrate. It has been hypothesized that excreted nitrite represents excess nitrogen cannot be further assimilated due to missing carbon, but underlying molecular mechanisms are unclear. Here, we identified protein interacts with reductase, regulates metabolism and promotes excretion. The protein, which named NirP1, encoded by an...
A spectroradiometer has been developed for direct measurement of the solar actinic UV flux (scalar intensity) and determination photolysis frequencies in atmosphere. The instrument is based on a scanning double monochromator with an entrance optic that exhibits isotropic angular response over solid angle 2pi sr. Actinic spectra are measured at resolution 1 nm across range 280-420 nm, which relevant most tropospheric processes. derived from radiation by use published absorption cross sections...
Epidemiological studies indicate that N-nitroso compounds (NOC) are causally linked to colorectal cancer (CRC). NOC induce DNA alkylations, including O (6)-methylguanine (O (6)-MeG) and N-methylated purines, which repaired by (6)-MeG-DNA methyltransferase (MGMT) N-alkyladenine-DNA glycosylase (AAG)-initiated base excision repair, respectively. In view of recent evidence nonlinear mutagenicity for NOC-like compounds, the question arises as existence threshold doses in CRC formation. Here, we...
Alkylating agents are present in food and tobacco smoke, but also used cancer chemotherapy, inducing the DNA lesion O6-methylguanine. This critical adduct is repaired by O6-methylguanine-DNA methyltransferase (MGMT), resulting MGMT inactivation degradation. In study, we analyzed effects of natural disulfide compound lipoic acid (LA) on vitro colorectal cells. We show that LA, not its reduced form dihydrolipoic acid, potently inhibits activity recombinant interfering with catalytic Cys-145...
The small RNA AbcR1 regulates the expression of ABC transporters in plant pathogen Agrobacterium tumefaciens, symbiont Sinorhizobium meliloti, and human Brucella abortus. A combination proteomic bioinformatic approaches suggested dozens targets A. tumefaciens. Several these newly discovered are involved uptake amino acids, their derivatives, sugars. Among latter is periplasmic sugar-binding protein ChvE, a component virulence signal transduction system. We examined 16 interaction with close...
Purpose: Ischemic preconditioning (IPC) has been used to protect myocardial cells against ischemiareperfusion injury and is recently utilized for improving exercise performance.It unknown whether remote IPC (RIPC) tissues not involved in can induce similar improvements what "dose" of necessary performance benefits.This study determined if unilateral bilateral upper limb RIPC improves lower body anaerobic power output.Methods: Using two randomized, sham-controlled, crossover designs, we...
PhIP is an abundant heterocyclic aromatic amine (HCA) and important dietary carcinogen.Following metabolic activation, causes bulky DNA lesions at the C8-position of guanine.Although C8-PhIP-dG adducts are mutagenic, their interference with replication machinery elicited damage response (DDR) have not yet been studied.Here, we analyzed PhIP-triggered replicative stress elucidated role apical DDR kinases ATR, ATM DNA-PK cs in cellular defense response.First, demonstrate that induced strand...
Abstract Throughout the tree of life RNA-binding proteins play important roles, but they are poorly characterized in cyanobacteria. Structural prediction suggests an interface for protein YlxR/Ssr1238 cyanobacterium Synechocystis 6803. Two pairs cysteine residues arranged as possibly coordinating Fe-S cluster and appear widely conserved homologous other Overexpression Ssr1238 24 h led to higher levels RNase P RNA, tRNAs, stress-related mRNAs. Co-immunoprecipitation followed by MS analysis...
Throughout the tree of life RNA-binding proteins play important roles, but they are poorly characterized in cyanobacteria. Overexpression predicted protein Ssr1238 cyanobacterium Synechocystis 6803 for 24 h led to higher levels RNase P RNA, tRNAs, and stress-related mRNAs. Co-immunoprecipitation followed by MS analysis sequencing UV crosslinked, co-immunoprecipitated RNA samples identified potential interaction partners Ssr1238. The most enriched transcript was RnpA, component P, among...
In any given organism, approximately one-third of all proteins have a yet-unknown function. A widely distributed domain unknown function is DUF1127. Approximately 17,000 with such an arginine-rich are found in 4,000 bacteria. Most them single-domain proteins, and large fraction qualifies as small fewer than 50 amino acids. We systematically identified characterized the seven DUF1127 members plant pathogen Agrobacterium tumefaciens They give rise to authentic differentially expressed shown at...
Small RNAs (sRNAs) are universal posttranscriptional regulators of gene expression and hundreds sRNAs frequently found in each every bacterium. In order to coordinate cellular processes response ambient conditions, many differentially expressed. Here, we asked how these small regulated using Agrobacterium tumefaciens as a model system. Among the best-studied this plant pathogen AbcR1 regulating numerous ABC transporters PmaR, regulator peptidoglycan biosynthesis, motility, ampicillin...
The trace element copper serves as cofactor for many enzymes but is toxic at elevated concentrations. In bacteria, the intracellular level maintained by efflux systems including Cue system controlled transcription factor CueR. CueR, a member of MerR family, forms homodimers and binds monovalent ions with high affinity. It activates tolerance genes copA cueO via conserved DNA-distortion mechanism. mechanism how CueR-induced turned off not fully understood. Here, we report that Escherichia...
Riboregulation involving regulatory RNAs, RNA chaperones, and ribonucleases is fundamental for the rapid adaptation of gene expression to changing environmental conditions. The coding RNase YbeY belongs minimal prokaryotic genome set has a profound impact on physiology in wide range bacteria. Here, we show that Agrobacterium tumefaciensybeY not essential. Deletion plant pathogen reduced growth, motility, stress tolerance. Most interestingly, crucial A. tumefaciens-mediated T-DNA transfer...
Abstract Diels-Alder reaction of 4-amino-o-quinodimethane (6) with [60]fullerene (1) affords the thermally stable and chemically reactive adduct 7(n). The nucleophilic amino group 7(n) is well suited for covalent attachment different molecules to core by further chemical reactions.
There is accumulating evidence that the subfamily of large-conductance potassium (“big”, “BK”) channels are involved in diverse, and perhaps coordinated, protective or counteractive responses to local generalized ischemia hypoxia. Although widely distributed, physiological differences among BK which results from posttranslational modification (alternative splicing) co-assembly with auxiliary modulatory subunits (β1-4 γ1-4), bestows localized subunit composition, distribution, 2nd-messenger...