A5051547409- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Biocrusts and Microbial Ecology
- Protist diversity and phylogeny
- Antibiotic Resistance in Bacteria
- Metabolomics and Mass Spectrometry Studies
- Plant Pathogenic Bacteria Studies
- Antioxidant Activity and Oxidative Stress
- Mitochondrial Function and Pathology
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Salmonella and Campylobacter epidemiology
- RNA Research and Splicing
- Infections and bacterial resistance
- Escherichia coli research studies
- Microbial infections and disease research
- Enterobacteriaceae and Cronobacter Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Aquaculture disease management and microbiota
- Marine and coastal ecosystems
University of Freiburg
2015-2024
Otto-von-Guericke University Magdeburg
2022-2023
Significance Carotenoids are indispensable to plants and humans. Despite significant achievements in carotenoid research, we still lack the fundamental knowledge of regulatory mechanisms underlying carotenogenesis plants. Phytoene synthase (PSY) ORANGE (OR) two key proteins for biosynthesis accumulation plastids. This study shows that OR family interact directly with PSY function as major regulators active protein abundance mediating biosynthesis. The findings establish posttranscriptional...
Oxygenic photosynthesis requires the coordination of environmental stimuli with regulation transcription. The transcription factor RpaB is conserved from simplest unicellular cyanobacteria to complex eukaryotic algae, representing more than 1 billion years evolution. To predict RpaB-controlled regulon in cyanobacterium Synechocystis, we analyzed positional distribution binding sites together high-resolution mapping data transcriptional start (TSSs). We describe 150 target promoters whose...
Abstract Although regulatory small RNAs have been reported in photosynthetic cyanobacteria, the lack of clear RNA chaperones involved their regulation poses a conundrum. Here, we analyzed full complement cellular and proteins using gradient profiling by sequencing (Grad-seq) Synechocystis 6803. Complexes with overlapping subunits such as CpcG1-type versus CpcL-type phycobilisomes or PsaK1 PsaK2 photosystem I pre(complexes) could be distinguished, supporting high quality this approach....
Summary Oxygenic photosynthesis evolved in cyanobacteria, primary producers of striking ecological importance. Like plants, cyanobacteria use the Calvin–Benson–Bassham cycle for CO 2 fixation, fuelled by ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCO). In a competitive reaction this enzyme also fixes O which makes it rather ineffective. To mitigate problem, concentrating mechanism (CCM) to pool vicinity RuBisCO. However, regulation these carbon (C) assimilatory systems is...
Abstract Throughout the tree of life RNA-binding proteins play important roles, but they are poorly characterized in cyanobacteria. Structural prediction suggests an interface for protein YlxR/Ssr1238 cyanobacterium Synechocystis 6803. Two pairs cysteine residues arranged as possibly coordinating Fe-S cluster and appear widely conserved homologous other Overexpression Ssr1238 24 h led to higher levels RNase P RNA, tRNAs, stress-related mRNAs. Co-immunoprecipitation followed by MS analysis...
Throughout the tree of life RNA-binding proteins play important roles, but they are poorly characterized in cyanobacteria. Overexpression predicted protein Ssr1238 cyanobacterium Synechocystis 6803 for 24 h led to higher levels RNase P RNA, tRNAs, and stress-related mRNAs. Co-immunoprecipitation followed by MS analysis sequencing UV crosslinked, co-immunoprecipitated RNA samples identified potential interaction partners Ssr1238. The most enriched transcript was RnpA, component P, among...
The Ferric uptake regulator (Fur) is crucial to both pathogenic and non-pathogenic bacteria for the maintenance of iron homeostasis as well defence against reactive oxygen species. Based on datasets from genome-wide mapping transcriptional start sites transcriptome data, we identified a high confidence regulon controlled by Fur model cyanobacterium Synechocystis sp. PCC 6803 its close relative, strain 6714, based conserved strong starvation response Fur-binding site occurrence. This...
Extended spectrum beta-lactamases (ESBL) are frequently found in Enterobacterales isolates from Western Africa. However, information on the molecular epidemiology of regional ESBL-positive strains is scarce. In order to provide epidemiological information, Escherichia coli stool samples European soldiers with diarrhea deployed a field camp Mali were subjected whole-genome sequencing (Illumina MiSeq and Oxford Nanopore MinION) antimicrobial susceptibility testing. With two exemptions,...
Erysipelothrix rhusiopathiae is a facultative anaerobic, environmentally stable, Gram-positive rod that causes swine and avian erysipelas as zoonotic pathogen. In humans, the main manifestations described are circumscribed erysipeloid, generalized endocarditis. Here, we report 46-year-old female patient who presented to physician because of redness marked functio laesa hand, in terms pain-related restricted range motion, was treated surgically. E. rhusopathiae detected tissue biopsy. The...
Chimaeribacter arupi (heterotypic synonym: "Nissabacter archeti") is a facultative anaerobic, newly described Gram-negative rod and belongs to the Yersineacea family. Here, we report case of 19-month-old female infant patient who presented emergency unit with somnolence fever. C. was isolated from positive blood culture, taken via an implanted Broviac catheter, proving bloodstream infection by pathogen. The objective this study utilize whole genome sequencing assess genes encoding potential...
Abstract Regulatory sRNAs in photosynthetic cyanobacteria have been reported, but the lack of plausible RNA chaperones involved this regulation appears enigmatic. Here, we analyzed full ensemble cellular RNAs and proteins using gradient profiling by sequencing (Grad-seq) Synechocystis 6803. Complexes with overlapping subunits such as CpcG1-type versus CpcL-type phycobilisomes or PsaK1 PsaK2 photosystem I pre(complexes) could be distinguished supporting a high quality approach. Clustering...
ABSTRACT The transcription factor RpaB regulates the expression of genes encoding photosynthesis-associated proteins during light acclimation. binding site is HLR1 motif, a pair imperfect octameric direct repeats, separated by two random nucleotides. Here, we used high-resolution mapping data transcriptional start sites (TSSs) in model Synechocystis sp. PCC 6803 conjunction with positional distribution for global prediction regulon. results demonstrate that more than 150 promoters, driving...
Abstract Microorganisms evolved specific acclimation strategies to thrive in environments of high or fluctuating salinities. Here, salt the model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by integrating transcriptomic, proteomic and metabolomic data. A dynamic reorganization transcriptome proteome occurred during first hours after shock, e.g. involving upregulation genes activate compatible solute biochemistry balancing osmotic pressure. The massive accumulation glucosylglycerol...