A5028213195- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Conducting polymers and applications
- Protist diversity and phylogeny
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Microbial Community Ecology and Physiology
- RNA Research and Splicing
- Molecular Junctions and Nanostructures
- Acoustic Wave Resonator Technologies
University of Freiburg
1993-2025
Abstract Although regulatory small RNAs have been reported in photosynthetic cyanobacteria, the lack of clear RNA chaperones involved their regulation poses a conundrum. Here, we analyzed full complement cellular and proteins using gradient profiling by sequencing (Grad-seq) Synechocystis 6803. Complexes with overlapping subunits such as CpcG1-type versus CpcL-type phycobilisomes or PsaK1 PsaK2 photosystem I pre(complexes) could be distinguished, supporting high quality this approach....
Abstract Many bacteria and archaea use CRISPR-Cas systems, which provide RNA-based, adaptive, inheritable immune defenses against invading viruses other foreign genetic elements. The proper processing of CRISPR guide RNAs (crRNAs) is a crucial step in the maturation defense complexes frequently performed by specialized ribonucleases encoded cas genes. However, some systems employ enzymes associated with degradosome or housekeeping functions, such as RNase III endoribonuclease E. Here, endo-...
RNA degradation is critical for synchronising gene expression with changing conditions in prokaryotic and eukaryotic organisms. In bacteria, the preference of central ribonucleases RNase E, J Y 5'-monophosphorylated RNAs considered important degradation. For underlying mechanism termed 5' sensing, contrasting to alternative 'direct entry' mode, which independent monophosphorylated ends. Cyanobacteria, such as Synechocystis sp. PCC 6803 (Synechocystis), encode E homologues. Here, we...
How CRISPR-Cas systems defend bacteria and archaea against invading genetic elements is well understood, but less known about their regulation. In the cyanobacterium Synechocystis sp. PCC 6803, expression of one three different responds to changes in environmental conditions. The cas operon promoter this system controlled by light- redox-responsive transcription factor RpaB binding an HLR1 motif, resulting transcriptional activation at low light intensities. However, strong that drives...
Abstract RNA degradation is crucial for many processes in pro- and eukaryotic organisms. In bacteria, the preference of central ribonucleases RNase E, J Y towards 5’-monophosphorylated RNAs considered important degradation. For underlying mechanism termed 5’ sensing. Cyanobacteria, such as Synechocystis sp. PCC 6803 ( ), encode E homologs. Here, we constructed a strain lacking sensing function mapped on transcriptome-wide level 292 5’-sensing-dependent cleavage sites. These included so far...
Abstract Regulatory sRNAs in photosynthetic cyanobacteria have been reported, but the lack of plausible RNA chaperones involved this regulation appears enigmatic. Here, we analyzed full ensemble cellular RNAs and proteins using gradient profiling by sequencing (Grad-seq) Synechocystis 6803. Complexes with overlapping subunits such as CpcG1-type versus CpcL-type phycobilisomes or PsaK1 PsaK2 photosystem I pre(complexes) could be distinguished supporting a high quality approach. Clustering...
ABSTRACT CRISPR‒Cas systems in bacteria and archaea provide powerful defense against phages other foreign genetic elements. The principles of activity are well understood, but less is known about how their expression regulated. cyanobacterium Synechocystis sp. PCC 6803 encodes three different systems. one these, a type III-Dv system, responds to changes environmental conditions, such as nitrogen starvation or varying light intensities. Here, we found that the promoter six-gene cas operon for...
Scanning tunneling microscopy (STM) becomes an excellent tool for surface structure studies in the case of conductive and semiconductive materials. The STM has advantage extending range SEM TEM to topography measurements angstrom scale. This method also does not require special sample preparations can be used air. polymers with electronic conductivity are among compounds studied by STM. Recently images doped polypyrrole were obtained polymer molecules deposited on graphite. authors claimed...