A5006120270- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Antioxidant Activity and Oxidative Stress
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Microbial Metabolic Engineering and Bioproduction
- DNA Repair Mechanisms
- Plant Micronutrient Interactions and Effects
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Lipid metabolism and biosynthesis
- Cocoa and Sweet Potato Agronomy
- Advances in Cucurbitaceae Research
- DNA and Nucleic Acid Chemistry
- Mitochondrial Function and Pathology
- Medical Research and Treatments
- Simulation Techniques and Applications
- Selenium in Biological Systems
- Military Strategy and Technology
- Bone health and treatments
- Advanced Decision-Making Techniques
- Employment and Welfare Studies
- Enzyme Production and Characterization
- Electrochemical sensors and biosensors
- Legume Nitrogen Fixing Symbiosis
Zhongnan University of Economics and Law
2025
Robert W. Holley Center for Agriculture & Health
2013-2023
Cornell University
2013-2023
Fudan University Shanghai Cancer Center
2022
United States Department of Agriculture
2015-2019
Agricultural Research Service
2019
Hubei University of Medicine
2017
Texas A&M University
2016
Shanghai Jiao Tong University
2013-2015
Huazhong Agricultural University
2015
Significance Carotenoids are indispensable to plants and humans. Despite significant achievements in carotenoid research, we still lack the fundamental knowledge of regulatory mechanisms underlying carotenogenesis plants. Phytoene synthase (PSY) ORANGE (OR) two key proteins for biosynthesis accumulation plastids. This study shows that OR family interact directly with PSY function as major regulators active protein abundance mediating biosynthesis. The findings establish posttranscriptional...
Summary The flesh color of Cucumis melo (melon) is genetically determined, and can be white, light green or orange, with β–carotene being the predominant pigment. We associated carotenoid accumulation in melon fruit polymorphism within CmOr , a homolog cauliflower BoOr gene, identified as previously described gf locus melon. was found to co‐segregate color, presented two haplotypes (alleles) broad germplasm collection, one orange second either white flesh. Allelic variation does not affect...
Summary Land plants contain a large family of genes that encode for pentatricopeptide (PPR) proteins. To date, few these PPR proteins have been functionally characterized. In this study, we analyzed an Arabidopsis mutant, slg1 , which exhibits slow growth and delayed development. addition, shows enhanced response to ABA increased tolerance drought stress. The SLG1 gene encodes protein is localized in mitochondria. the RNA editing single site mitochondrial transcript nad3 abolished. subunit...
β-Carotene adds nutritious value and determines the color of many fruits, including melon (Cucumis melo). In mesocarp, β-carotene accumulation is governed by Orange gene (CmOr) golden single-nucleotide polymorphism (SNP) through a yet to be discovered mechanism. Arabidopsis (Arabidopsis thaliana), OR increases carotenoid levels posttranscriptionally regulating phytoene synthase (PSY). Here, we identified CmOr nonsense mutation (Cmor-lowβ) that lowered fruit with impaired chromoplast...
Plastid ribosomal proteins are essential components of protein synthesis machinery and have diverse roles in plant growth development. Mutations plastid lead to a range developmental phenotypes plants. However, how they regulate these processes is not fully understood, the functions some individual remain unknown. To identify genes responsible for chloroplast development, we isolated characterized mutant that exhibited pale yellow inner leaves with reduced rate Arabidopsis. The (rps5)...
Carotenoids are crucial for plant growth and human health. The finding of ORANGE (OR) protein as a pivotal regulator carotenogenesis offers unique opportunity to comprehensively understand the regulatory mechanisms carotenoid accumulation develop crops with enhanced nutritional quality. Here, we demonstrated that alteration single amino acid in wild-type OR greatly its ability promote accumulation. Whereas overexpression from Arabidopsis (Arabidopsis thaliana; AtOR) or agronomically...
Carotenoids are critically important to plants and humans. The ORANGE (OR) gene is a key regulator for carotenoid accumulation, but its physiological roles in crops remain elusive. In this study, we generated transgenic tomato ectopically overexpressing the Arabidopsis wild-type OR (AtORWT ) 'golden SNP'-containing (AtORHis ). We found that AtORHis initiated chromoplast formation very young fruit stimulated accumulation at all developmental stages, uncoupled from other ripening activities....
Chromoplasts are unique plastids that accumulate massive amounts of carotenoids. To gain a general and comparative characterization chromoplast proteins, this study performed proteomic analysis chromoplasts from six carotenoid-rich crops: watermelon, tomato, carrot, orange cauliflower, red papaya, bell pepper. Stromal membrane proteins were separated by 1D gel electrophoresis analysed using nLC-MS/MS. A total 953–2262 different crop species identified. Approximately 60% the identified...
Carotenoids exert multifaceted roles to plants and are critically important humans. Phytoene synthase (PSY) is a major rate-limiting enzyme in the carotenoid biosynthetic pathway. PSY normally found as small family with up three members. However, knowledge of isoforms relation their respective activities amino acid residues that for activity limited. In this study, we focused on two tomato (
Selenium (Se)-enriched broccoli has health-beneficial selenium-containing compounds, but it may contain reduced amounts of chemopreventive glucosinolates. To investigate the basis by which Se treatment influences glucosinolate levels, we treated two cultivars with 25 μM Na2SeO4. We found that supplementation suppressed accumulation total glucosinolates, particularly glucoraphanin, direct precursor a potent anticancer compound, in florets and leaves. showed suppression was not associated...
Melon fruit flesh color is primarily controlled by the "golden" single nucleotide polymorhism of "Orange" gene, CmOr, which dominantly triggers accumulation pro-vitamin A molecule, β-carotene, in mesocarp. The mechanism CmOr operates not fully understood. To identify cellular and metabolic processes associated with allelic variation, we compared transcriptome bulks developing homozygous orange green fruited F3 families derived from a cross between parental lines. Pooling together that share...
Patient moral hazard is a significant issue in healthcare system reform and prominent factor affecting the efficiency of services China. Based on consolidation urban rural resident health insurance, this paper employs staggered DID model to analyze impact patient service utilization. The findings are as follows. First, utilization significantly increases after insurance. This conclusion remains robust when subjected placebo test, mitigation non-random policy effects, exclusion other...
Abstract Carotenoids, such as β-carotene, accumulate in chromoplasts of various fleshy fruits, awarding them with colors, aromas, and nutrients. The Orange ( CmOr ) gene controls β-carotene accumulation melon fruit by posttranslationally enhancing carotenogenesis repressing turnover chromoplasts. Carotenoid isomerase (CRTISO) isomerizes yellow prolycopene into red lycopene, a prerequisite for further metabolism β-carotene. We comparatively analyzed the developing transcriptomes...
Chromoplasts are plant organelles with a unique ability to sequester and store massive carotenoids. have been hypothesized enable high levels of carotenoid accumulation due enhanced sequestration or substructure formation. However, the regulators that control component formation in chromoplasts remain unknown. In melon (Cucumis melo) fruit, β-carotene is governed by ORANGE (OR), key regulator for chromoplasts. By using comparative proteomic analysis variety its isogenic line low-β mutant...
Cauliflower Orange (Or) mutant is characterized by high levels of -carotene in its curd. Or mutation affects the OR protein that was shown to be involved posttranslational control phytoene synthase (PSY), a major rate-limiting enzyme carotenoid biosynthesis, and maintaining PSY proteostasis with plastid Clp protease system. A transposon integration into cauliflower gene (BoOR) results formation three differently spliced transcripts. One them insertion (BoOR-Ins), while other two have...