A5018430040- Photosynthetic Processes and Mechanisms
- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- Plant Parasitism and Resistance
- Gene Regulatory Network Analysis
- Vitamin C and Antioxidants Research
- Plant and animal studies
- Molecular Communication and Nanonetworks
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Fungal Plant Pathogen Control
- Cancer, Hypoxia, and Metabolism
- Plant responses to elevated CO2
- 3D Printing in Biomedical Research
- Plant Molecular Biology Research
University of Freiburg
2015-2022
The Arabidopsis carotenoid cleavage dioxygenase 4 (AtCCD4) is a negative regulator of the content seeds and has recently been suggested as candidate for generation retrograde signals that are thought to derive from poly-cis-configured carotene desaturation intermediates. In this work, we investigated activity AtCCD4 in vitro used dynamic modeling determine its substrate preference. Our results document strict regional specificity at C9-C10 double bond carotenoids apocarotenoids, with...
Geranylgeranyl diphosphate (GGPP), a prenyl synthesized by GGPP synthase (GGPS), represents metabolic hub for the synthesis of key isoprenoids, such as chlorophylls, tocopherols, phylloquinone, gibberellins, and carotenoids. Protein-protein interactions amphipathic nature suggest metabolite channeling and/or competition among enzymes that function in independent branches isoprenoid pathway. To investigate substrate conversion efficiency between plastid-localized GGPS isoform GGPS11 phytoene...
Provitamin A biofortification, the provision of provitamin carotenoids through agriculture, is regarded as an effective and sustainable intervention to defeat vitamin deficiency, representing a global health problem. This food-based has been questioned in conjunction with negative outcomes for smokers asbestos-exposed populations CARET ATBC trials which very high doses β-carotene were supplemented. The current notion that cleavage products (apocarotenoids) represented harmful agents basis...
Phytoene desaturase (PDS) is an essential plant carotenoid biosynthetic enzyme and a prominent target of certain inhibitors, such as norflurazon, acting bleaching herbicides. PDS catalyzes the introduction two double bonds into 15-cis-phytoene, yielding 9,15,9'-tri-cis-ζ-carotene via intermediate 9,15-di-cis-phytofluene. We present necessary data to scrutinize functional implications inferred from recently resolved crystal structure Oryza sativa in complex with norflurazon. Using dynamic...
Cauliflower Orange (Or) mutant is characterized by high levels of -carotene in its curd. Or mutation affects the OR protein that was shown to be involved posttranslational control phytoene synthase (PSY), a major rate-limiting enzyme carotenoid biosynthesis, and maintaining PSY proteostasis with plastid Clp protease system. A transposon integration into cauliflower gene (BoOR) results formation three differently spliced transcripts. One them insertion (BoOR-Ins), while other two have...
Abstract Carotenoid levels in plant tissues depend on the relative rates of synthesis and degradation molecules pathway. While carotenoid biosynthesis has been extensively characterized, research catabolism into apocarotenoids is a relatively novel field. To identify apocarotenoid metabolic processes, we characterized transcriptome transgenic Arabidopsis (Arabidopsis thaliana) roots accumulating high β-carotene and, consequently, β-apocarotenoids. Transcriptome analysis revealed feedback...
Recombinant phytoene desaturase (PDS-His6) from rice was purified to near-homogeneity and shown be enzymatically active in a biphasic, liposome-based assay system. The protein contains FAD as the sole protein-bound redox-cofactor. Benzoquinones, not replaceable by molecular oxygen, serve final electron acceptor defining PDS 15-cis-phytoene (donor):plastoquinone oxidoreductase. herbicidal PDS-inhibitor norflurazon is capable of arresting reaction stabilizing intermediary FADred, while an...
Abstract Synthetic biology applies engineering concepts to build cellular systems that perceive and process information. This is achieved by assembling genetic modules according design principles. Recent advance in the field has contributed optogenetic switches for controlling diverse biological functions response light. Here, concept introduced apply synthetic principles synthesis of multi‐input‐processing materials. exemplified a materials system counts light pulses. Guided quantitative...
Analysis of carotenoid-accumulating roots revealed that oxidative carotenoid degradation yields glyoxal and methylglyoxal. Our data suggest these compounds are detoxified via the glyoxalase system re-enter primary metabolic pathways. Carotenoid levels in plant tissues depend on relative rates synthesis degradation. We recently identified redox enzymes previously known to be involved detoxification fatty acid-derived reactive carbonyl species which were able convert apocarotenoids into...
Based on synthetic biology-derived molecular tools and design concepts, in article number 1800472, Wilfried Weber co-workers present biohybrid material systems that count the of light pulses release different output molecules as a function detected. Using quantitative mathematical model, input configurations can efficiently be identified yielding desired system outputs. Cover image designed by Michal Rössler.