A5012795750- Probiotics and Fermented Foods
- Antioxidant Activity and Oxidative Stress
- Photosynthetic Processes and Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Agriculture and Rural Development Research
- Nutrition, Genetics, and Disease
- Infant Nutrition and Health
- Psychology, Coaching, and Therapy
- Phytochemistry and Bioactivity Studies
- Fatty Acid Research and Health
- interferon and immune responses
- Microbial Metabolites in Food Biotechnology
- Digestive system and related health
- Consumer Attitudes and Food Labeling
- IL-33, ST2, and ILC Pathways
- GABA and Rice Research
- Proteins in Food Systems
- Bioactive Compounds and Antitumor Agents
Agroscope
2019-2024
University of Bern
2022
SIB Swiss Institute of Bioinformatics
2022
University of Freiburg
2008-2018
Abstract Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production accumulation fight vitamin deficiency disorders. We show here that allelic polymorphism one two expressed phytoene synthase (PSY) genes capable enhancing flux carbon through carotenogenesis, thus...
We here report on the characterization of a novel third phytoene synthase gene (PSY) in rice (Oryza sativa), OsPSY3, and differences among all three PSY genes with respect to tissue-specific expression regulation upon various environmental stimuli. The two already known PSYs are under phytochrome control involved carotenoid biosynthesis photosynthetically active tissues exhibit different patterns during chloroplast development. In contrast, OsPSY3 transcript levels not affected by light show...
Geranylgeranyl diphosphate (GGPP), a prenyl synthesized by GGPP synthase (GGPS), represents metabolic hub for the synthesis of key isoprenoids, such as chlorophylls, tocopherols, phylloquinone, gibberellins, and carotenoids. Protein-protein interactions amphipathic nature suggest metabolite channeling and/or competition among enzymes that function in independent branches isoprenoid pathway. To investigate substrate conversion efficiency between plastid-localized GGPS isoform GGPS11 phytoene...
While proteomic techniques allow the identification and relative quantification of thousands proteins in a single run, methods for absolute remain laborious. In this study, newly developed multiple reaction monitoring (MRM) method using liquid chromatography mass spectrometry (LC-MS) that enables simultaneous twenty key milk is presented. The selected comprise all individual caseins, major whey most well-known fat globule membrane (MFGM) proteins. For validation, raw milk, cream, Emmental...
Impact of herbage proportion, breed, average days in lactation (ADiL), and season on bulk milk composition 12 dairy farms were investigated over a year using mixed effect model approach. A 10%-points higher proportion led to contents omega 3 (n-3) fatty acids (FAs), conjugated linoleic (CLA), vaccenic acid, branched chain FAs (+0.08, +0.08, +0.19, +0.05 g 100 g−1 fat, respectively). Breed influenced n-3 CLA (+0.31, +0.17 respectively), κ-casein, lipoprotein lipase, β-lactoglobulin, total...
Abstract Unraveling bacterial gene function drives progress in various areas, such as food production, pharmacology, and ecology. While omics technologies capture high-dimensional phenotypic data, linking them to genomic data is challenging, leaving 40–60% of genes undescribed. To address this bottleneck, we introduce Scoary2 , an ultra-fast microbial genome-wide association studies (mGWAS) software. With its exploration app improved performance, the first tool enable study large datasets...
Mycobacterium tuberculosis, the causative agent of is assumed to lack carotenoids, which are widespread pigments fulfilling important functions as radical scavengers and a source apocarotenoids. In mammals, synthesis apocarotenoids, including retinoic acid, initiated by β-carotene cleavage oxygenases I II catalyzing either central or an excentric β-carotene, respectively. The M. tuberculosis ORF Rv0654 codes for putative carotenoid oxygenase conserved in other mycobacteria. present study, we...
The diversity of the human microbiome is positively associated with health. However, this endangered by Westernized dietary patterns that are characterized a decreased nutrient variety. Diversity might potentially be improved promoting rich in microbial strains. Various collections bacterial cultures resulting from century dairy research readily available worldwide, and could exploited to contribute towards end. We have conducted functional silico analysis metagenome 24 strains, each...
ABSTRACT Indole derivatives are microbial metabolites of the tryptophan pathway involved in gut immune homeostasis. They bind to aryl hydrocarbon receptor (AhR), thereby modulating development intestinal group 3 innate lymphoid cells (ILC3) and subsequent interleukin-22 production. In mice, indole maternal microbiota can reach milk drive early postnatal ILC3 development. Apart from microbiota, lactic acid bacteria (LAB) also produce compounds during fermentation. Using germ-free aim our...
Abstract Genomic screening of bacteria is common practice to select strains with desired properties. However, 40-60% all bacterial genes are still unknown, making capturing the phenotype an important part selection process. While omics-technologies collect high-dimensional phenotypic data, it remains challenging link this information genomic data elucidate impact specific on phenotype. To end, we present Scoary2, ultra-fast software for microbial genome-wide association studies (mGWAS),...
The function of the aminotransferase Aat (GenBank Protein WP_159211138) from Pediococcus acidilactici FAM 18098 was studied in vivo. For this purpose, gene replaced with an erythromycin resistance using temperature-sensitive Escherichia coli-Pediococcus shuttle plasmid pSET4T_Δaat. knockout verified by PCR and genome sequencing. Subsequently, differences between metabolism wild-type strain were investigated determining free amino acids organic culture supernatants. It found that mutant no...
Huge numbers of bacteria live in the human gut. We know those are important to our health, so we need treat them well. wanted whether it was possible design new yogurts that can introduce special into gut, improve well-being. studied hundreds types isolated from cheese and yogurt found 24 these bacterial species perform most functions happen Therefore, there is exciting potential for designing new, gut-healthy yogurts.