ABSTRACT Legg-Calvé-Perthes disease (LCPD) is a childhood hip disorder of ischemic osteonecrosis the femoral head. Hip joint synovitis common feature LCPD, but nature and pathophysiology remain unknown. The purpose this study was to determine chronicity inflammatory cytokines present in synovial fluid at an active stage LCPD. Serial MRI performed on 28 patients. T2-weighted gadolinium-enhanced MR images were used assess effusion enhancement (hyperemia) over time. A multiple-cytokine assay...

Local non-weight-bearing as a treatment for Legg-Calvé-Perthes disease remains controversial since clear scientific basis this is lacking. The purpose of study was to determine the effects on decreasing femoral head deformity following ischemic osteonecrosis and investigate its biological effects.Unilateral ischemia induced in sixteen piglets by placing ligature around neck transecting ligamentum teres. Eight animals received hind-limb amputation prevent weight-bearing side (NWB group)....

Background: Non-weight-bearing decreases the femoral head deformity but increases bone resorption without increasing formation in an experimental animal model of Legg-Calvé-Perthes disease. We sought to determine if local administration morphogenetic protein (BMP)-2 with or bisphosphonate can increase during non-weight-bearing treatment large Methods: Eighteen piglets were surgically induced ischemia. Immediately following surgery, all animals received above-the-knee amputation enforce...

Genetic engineering techniques were used to develop an animal model of juvenile scoliosis during a postnatal skeletal-growth stage.To investigate the effect targeted SHP2 (Src homology-2) deficiency in chondrocytes on development growth stage mice.Juvenile idiopathic can lead progressive severe spinal deformity. The pathophysiology and molecular mechanisms responsible for deformity are unknown. Here, we investigated role as potential cause scoliosis.Genetically engineered mice with inducible...

ABSTRACT Legg-Calvé-Perthes disease (LCPD) is a juvenile form of ischemic femoral head osteonecrosis, which produces chronic hip synovitis, permanent deformity, and premature osteoarthritis. Currently, there no medical therapy for LCPD. Interleukin-6 (IL-6) significantly elevated in the synovial fluid patients with We hypothesize that IL-6 elevation promotes synovitis impairs bone healing after osteonecrosis. set out to test if anti-IL-6 using tocilizumab can decrease improve piglet model...

Availability of a reliable mouse model ischemic osteonecrosis could accelerate the development novel therapeutic strategies to stimulate bone healing after osteonecrosis; however, no is currently available.To develop surgical osteonecrosis, we asked, (1) if blood vessels that contribute supply distal femoral epiphysis are cauterized, can generate an model; (2) what histologic changes observed in this model, and (3) morphologic model.We performed microangiography identify supplying mice, four...

Neurofibromatosis type 1 (NF1, OMIM 162200), caused by NF1 gene mutations, exhibits multi-system abnormalities, including skeletal deformities in humans. Osteocytes play critical roles controlling bone modeling and remodeling. However, the role of neurofibromin, protein product gene, osteocytes is largely unknown. This study investigated neurofibromin disrupting Nf1 under Dmp1-promoter. The conditional knockout (Nf1 cKO) mice displayed serum profile a metabolic disorder with an...

Bone morphogenetic protein (BMP)-2 and ibandronate (IB) decrease the femoral head deformity following ischemic osteonecrosis of (ONFH). The purpose this study was to determine effects BMP-2 IB on mineral content nanoindentation properties bone ONFH. ONFH surgically induced in piglets. There were five groups: normal control, untreated, IB, BMP, BMP + (n = 5/group). Backscattered electron imaging, Raman spectroscopy, testing performed. Both groups showed calcium trabecular similar group, while...

Background Legg‐Calvé‐Perthes disease (LCPD) is a childhood hip disorder thought to be caused by disruption of blood supply the developing femoral head. There potential for imaging help assess revascularization Purpose To investigate whether quantitative susceptibility mapping (QSM) can detect neovascularization in epiphyseal cartilage following ischemic injury Study Type Prospective. Animal Model Right head ischemia was surgically induced 6‐week‐old male piglets. The animals were sacrificed...

Purpose To determine whether quantitative MRI relaxation time mapping techniques can help to detect ischemic injury the developing femoral head. Materials and Methods For this prospective animal study conducted from November 2015 February 2018, 10 male 6-week-old piglets underwent an operation induce complete right head ischemia. Animals were humanely killed at 48 hours (n = 2) or 4 weeks 8) after operation, operated contralateral-control heads harvested frozen. Thawed specimens imaged 9.4-T...

Background: Two operative procedures are currently advocated to stimulate the necrotic femoral head healing in children with Legg-Calve-Perthes disease: transphyseal neck-head tunneling (TNHT) and multiple epiphyseal drilling (MED). The purpose of this study was compare bone physeal function after treatment TNHT or MED a piglet model ischemic osteonecrosis. Methods: Eighteen piglets were induced osteonecrosis by surgically placing ligature tightly around right neck. One week later, assigned...

Legg-Calvé-Perthes disease is juvenile idiopathic osteonecrosis of the femoral head (ONFH) that has no effective clinical treatment. Previously, local injection bone morphogenetic protein-2 (BMP2) for ONFH treatment showed a heterogeneous repair and high incidence heterotopic ossification (HO) due to BMP2 leakage. Here, we developed BMP2-hydrogel via transphyseal wash subsequential BMP2-loaded hydrogel. In vitro studies hydrogel gelatin-heparin-tyramine retained four weeks. The can...

Background: Ischemic osteonecrosis of the femoral head produces necrotic cell debris and inflammatory molecules in marrow space, which elicit a chronic repair response. The purpose this study was to determine effects flushing out proteins on bone piglet model ischemic osteonecrosis. Methods: Osteonecrosis right hindlimb induced 12 piglets by tying ligature tightly around neck. One week after surgery, 6 animals were treated with percutaneous 3-needle washing procedure non-weight-bearing (NWB)...

Legg-Calve-Perthes disease (LCPD) is a childhood form of ischemic osteonecrosis marked by development severe femoral head deformity and premature osteoarthritis. The pathogenesis has been studied extensively using piglet model osteonecrosis, however, accompanying acetabular changes have not investigated. purpose this study was to determine if accompany in well-established LCPD define the three dimensional computed tomography (3-D CT) modeling. Twenty-four piglets were surgically induced with...

Temperature elevation, a classic marker of infection and local temperature might be useful predictor early infection. However measurement around the spine is not readily accessible. The purpose this study was to explore whether sensing implant reproducibly detect change associated with peri-implant wound infection, in rabbit model.Twelve adult rabbits were implanted spinal screw-rod construct. probes placed at implantation site as well separate scapular away from surgical serve control....

Legg-Calvé-Perthes disease is juvenile idiopathic osteonecrosis of the femoral head (ONFH) that has no effective clinical resolutions. Previously, local injection bone morphogenetic protein-2 (BMP2) for ONFH treatment showed a heterogeneous repair and high incidence heterotopic ossification (HO) due to BMP2 leakage. Here, we developed BMP2-hydrogel via transphyseal wash subsequential BMP2-loaded hydrogel. In vivo studies hydrogel gelatin-heparin-tyramine retained four weeks. The can...

Decreased weight-bearing (WB) is commonly recommended to treat lower limb bone and joint disorders. The availability of a large animal model decreased or non–weight–bearing (NWB) would be invaluable study its effects on healing. We report here the development piglet NWB following induction ischemic osteonecrosis femoral head. purpose this was assess feasibility, effectiveness, complications various methods induce hind piglets. used decrease WB evolved from an Ehmer sling patellar and/or...