A5058586109- Advances in Cucurbitaceae Research
- Cocoa and Sweet Potato Agronomy
- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- Postharvest Quality and Shelf Life Management
- Agricultural Practices and Plant Genetics
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- Pineapple and bromelain studies
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Coconut Research and Applications
- Banana Cultivation and Research
- Agriculture, Soil, Plant Science
- Horticultural and Viticultural Research
- Ecology and Conservation Studies
- GABA and Rice Research
- Soybean genetics and cultivation
- Agronomic Practices and Intercropping Systems
- Plant nutrient uptake and metabolism
- Plant Genetic and Mutation Studies
- Plant Pathogenic Bacteria Studies
Agricultural Research Organization
2015-2025
The unique aroma of melons (Cucumis melo L., Cucurbitaceae) is composed many volatile compounds biosynthetically derived from fatty acids, carotenoids, amino and terpenes. Although acids are known precursors in the plant kingdom, initial steps catabolism into volatiles have received little attention. Incubation melon fruit cubes with α-keto led to enhanced formation bearing side chain exogenous or keto acid supplied. Moreover, L-[13C6]phenylalanine was also incorporated aromatic compounds....
Summary The flesh color of Cucumis melo (melon) is genetically determined, and can be white, light green or orange, with β–carotene being the predominant pigment. We associated carotenoid accumulation in melon fruit polymorphism within CmOr , a homolog cauliflower BoOr gene, identified as previously described gf locus melon. was found to co‐segregate color, presented two haplotypes (alleles) broad germplasm collection, one orange second either white flesh. Allelic variation does not affect...
The Cucurbitaceae family (cucurbit) includes several economically important crops, such as melon, cucumber, watermelon, pumpkin, squash and gourds. During the past years, genomic genetic data have been rapidly accumulated for cucurbits. To store, mine, analyze, integrate disseminate these large-scale datasets to provide a central portal cucurbit research breeding community, we developed Cucurbit Genomics Database (CuGenDB; http://cucurbitgenomics.org) using Tripal toolkit. database currently...
β-Carotene adds nutritious value and determines the color of many fruits, including melon (Cucumis melo). In mesocarp, β-carotene accumulation is governed by Orange gene (CmOr) golden single-nucleotide polymorphism (SNP) through a yet to be discovered mechanism. Arabidopsis (Arabidopsis thaliana), OR increases carotenoid levels posttranscriptionally regulating phytoene synthase (PSY). Here, we identified CmOr nonsense mutation (Cmor-lowβ) that lowered fruit with impaired chromoplast...
Color and pigment contents are important aspects of fruit quality consumer acceptance cucurbit crops. Here, we describe the independent mapping cloning a common causative APRR2 gene regulating accumulation in melon watermelon. We initially show that transcription factor is for qualitative difference between dark light green rind both Further analyses establish link sequence or expression level variations CmAPRR2 content flesh mature fruits. A genome-wide association study (GWAS) young color...
Carotenoids are crucial for plant growth and human health. The finding of ORANGE (OR) protein as a pivotal regulator carotenogenesis offers unique opportunity to comprehensively understand the regulatory mechanisms carotenoid accumulation develop crops with enhanced nutritional quality. Here, we demonstrated that alteration single amino acid in wild-type OR greatly its ability promote accumulation. Whereas overexpression from Arabidopsis (Arabidopsis thaliana; AtOR) or agronomically...
Sulfur-containing aroma volatiles are important contributors to the distinctive of melon and other fruits. Melon cultivars accessions differ in content sulfur-containing volatiles. L-methionine has been postulated serve as a precursor these Incubation fruit cubes with ¹³C- ²H-labeled revealed two distinct catabolic routes into One route apparently involves action an aminotransferase preserves main carbon skeleton L-methionine. The second L-methionine-γ-lyase activity, releasing methanethiol,...
External color has profound effects on acceptability of agricultural products by consumers. Carotenoids and chlorophylls are known to be the major pigments melon (Cucumis melo L.) rinds. Flavonoids (especially chalcones anthocyanins) also prominent in other fruits but have not been reported occur melons fruit. We analyzed accumulating rinds different genotypes during fruit development. found that rind is based combinations chlorophyll, carotenoids, flavonoids according cultivar tested their...
The flavonoids are phenylpropanoid-derived metabolites that ubiquitous in plants, playing many roles growth and development. Recently, we observed fruit rinds of yellow casaba muskmelons (Cucumis melo 'Inodorous Group') accumulate naringenin chalcone, a flavonoid pigment. With RNA-sequencing analysis bulked segregants representing the tails population segregating for chalcone accumulation followed by fine mapping genetic transformation, identified Kelch domain-containing F-box protein coding...
Cucumis melo is highly diverse for fruit traits providing wide breeding and genetic research opportunities, including genome-wide association (GWA) analysis. We used a collection of 177 accessions representing the two C. subspecies 11 horticultural groups detailed characterization variation evaluation potential GWA trait mapping in melon. Through genotyping-by-sequencing, 23,931 informative SNPs were selected analyses. found that linkage-disequilibrium decays at ~100 Kb this population...
Melon fruit flesh color is primarily controlled by the "golden" single nucleotide polymorhism of "Orange" gene, CmOr, which dominantly triggers accumulation pro-vitamin A molecule, β-carotene, in mesocarp. The mechanism CmOr operates not fully understood. To identify cellular and metabolic processes associated with allelic variation, we compared transcriptome bulks developing homozygous orange green fruited F3 families derived from a cross between parental lines. Pooling together that share...
Melon (Cucumis melo) fruits exhibit phenotypic diversity in several key quality determinants such as taste, color and aroma. Sucrose, carotenoids volatiles are recognized the compounds shaping above corresponding traits yet full network of biochemical events underlying their synthesis have not been comprehensively described. To delineate cellular processes fruit phenotypes, a population recombinant inbred lines (RIL) was used source genotypic variations. In parallel, ripe were analyzed for...
Abstract Carotenoids, such as β-carotene, accumulate in chromoplasts of various fleshy fruits, awarding them with colors, aromas, and nutrients. The Orange ( CmOr ) gene controls β-carotene accumulation melon fruit by posttranslationally enhancing carotenogenesis repressing turnover chromoplasts. Carotenoid isomerase (CRTISO) isomerizes yellow prolycopene into red lycopene, a prerequisite for further metabolism β-carotene. We comparatively analyzed the developing transcriptomes...
SUMMARY Linking genotype with phenotype is a fundamental goal in biology and requires robust data for both. Recent advances plant‐genome sequencing have expedited comparisons among multiple‐related individuals. The abundance of structural genomic within‐species variation that has been discovered indicates single reference genome cannot represent the complete sequence diversity species, leading to expansion pan‐genome concept. For high‐resolution forward genetics, this unprecedented access...
Chromoplasts are plant organelles with a unique ability to sequester and store massive carotenoids. have been hypothesized enable high levels of carotenoid accumulation due enhanced sequestration or substructure formation. However, the regulators that control component formation in chromoplasts remain unknown. In melon (Cucumis melo) fruit, β-carotene is governed by ORANGE (OR), key regulator for chromoplasts. By using comparative proteomic analysis variety its isogenic line low-β mutant...
Abstract Earliness and ripening behavior are important attributes of fruits on off the vine, affect quality preference both growers consumers. Fruit is a complex physiological process that involves metabolic shifts affecting fruit color, firmness, aroma production. Melon promising model crop for study ripening, as full spectrum climacteric represented across natural variation. Using Recombinant Inbred Lines (RILs) population derived from parental lines “Dulce” (reticulatus, climacteric) “Tam...
The melon ( Cucumis melo L.) fruit is an important crop and model system for the genomic study of both fleshy development Cucurbitaceae family. To obtain accurate representation transcriptome based on expressed sequence tag (EST) abundance in 454‐pyrosequencing data, we prepared double‐stranded complementary DNA (cDNA) without usual amplification normalization steps. A purification step was also included to eliminate small fragments. Complementary DNAs were obtained from 14 individual...
Induced mutagenesis of agricultural crops creates new variation in genes, including those affecting agriculturally important traits such as plant morphology and fruit quality. Melon (Cucumis melo L.) is a diploid species (2n = 24) with small genome, estimated 450 Mb, but relatively high levels sequence shape polymorphism. We treated seeds ‘Noy Yizre'el’, ‘Galia’ melon-type parental line, the chemical mutagen EMS (ethyl methane sulfonate). The resulting M1 plants were self-pollinated to...