- Advances in Cucurbitaceae Research
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Postharvest Quality and Shelf Life Management
- Cocoa and Sweet Potato Agronomy
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Powdery Mildew Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Resistance
- Tea Polyphenols and Effects
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Carcinogens and Genotoxicity Assessment
- Plant Physiology and Cultivation Studies
- Light effects on plants
- Microbial infections and disease research
- Photosynthetic Processes and Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Chinese history and philosophy
- Escherichia coli research studies
Beijing Academy of Agricultural and Forestry Sciences
2015-2024
China Agricultural University
2004-2024
China Tobacco
2023-2024
Maastricht University
2022
Beijing University of Chinese Medicine
2022
Second Military Medical University
2022
Shanghai Changzheng Hospital
2022
Boston Medical Center
2022
University Medical Center
2022
Boston University
2022
Zhangjun Fei and colleagues report the draft genome of a Chinese elite watermelon inbred line 97103 resequencing 20 diverse accessions that represent three subspecies Citrullus lunatus. Comparative genome-wide analyses identify extent genetic diversity population structure germplasm. Watermelon, lanatus, is an important cucurbit crop grown throughout world. Here we high-quality sequence east Asia cultivar (2n = 2× 22) containing 23,440 predicted protein-coding genes. genomics analysis...
The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and moschata provide evidence supporting an allotetraploidization event Cucurbita. We are able to partition into two homoeologous subgenomes based on different genetic distances melon, cucumber, watermelon Benincaseae tribe. estimate that diploid progenitors successively diverged from around 31 26 million years ago (Mya), respectively,...
Fruit characteristics of sweet watermelon are largely the result human selection. Here we report an improved reference genome and whole-genome resequencing 414 accessions representing all extant species in Citrullus genus. Population genomic analyses reveal evolutionary history Citrullus, suggesting independent evolutions amarus lineage containing lanatus mucosospermus. Our findings indicate that different loci affecting fruit size have been under selection during speciation, domestication...
The Cucurbitaceae family (cucurbit) includes several economically important crops, such as melon, cucumber, watermelon, pumpkin, squash and gourds. During the past years, genomic genetic data have been rapidly accumulated for cucurbits. To store, mine, analyze, integrate disseminate these large-scale datasets to provide a central portal cucurbit research breeding community, we developed Cucurbit Genomics Database (CuGenDB; http://cucurbitgenomics.org) using Tripal toolkit. database currently...
Abstract The Cucurbitaceae (cucurbit) family consists of about 1,000 species in 95 genera, including many economically important and popular fruit vegetable crops. During the past several years, reference genomes have been generated for >20 cucurbit species, variome transcriptome profiling data rapidly accumulated cucurbits. To efficiently mine, analyze disseminate these large-scale datasets, we developed an updated version Cucurbit Genomics Database. database, CuGenDBv2...
Abstract Background Cucumber, Cucumis sativus L., is an economically and nutritionally important crop of the Cucurbitaceae family has long served as a primary model system for sex determination studies. Recently, sequencing its whole genome been completed. However, transcriptome information this species still scarce, with total around 8,000 Expressed Sequence Tag (EST) mRNA sequences currently available in GenBank. In order to gain more insights into molecular mechanisms plant provide...
Abstract Background A number of molecular marker linkage maps have been developed for melon ( Cucumis melo L .) over the last two decades. However, these were constructed using different sets, thus, making comparative analysis among difficult. In order to solve this problem, a consensus genetic map in was primarily highly transferable anchor markers that broad potential use mapping, synteny, and quantitative trait loci (QTL) analysis, increasing breeding effectiveness efficiency via...
As part of our ongoing efforts to sequence and map the watermelon (Citrullus spp.) genome, we have constructed a high density genetic linkage map. The positioned 234 genome scaffolds (an average size 1.41 Mb) that cover about 330 Mb account for 93.5% 353 assembled genomic sequences elite Chinese line 97103 lanatus var. lanatus). was using an F8 population 103 recombinant inbred lines (RILs). RILs are derived from cross between United States Plant Introduction (PI) 296341-FR (C. citroides)...
How sugar transporters regulate accumulation in fruits is poorly understood and particularly so for species storing high-concentration Suc. Accumulation of soluble sugars watermelon (Citrullus lanatus) fruit, a major quality trait, had been selected during domestication. Still, the molecular mechanisms controlling this quantitative trait are unknown. We resequenced 96 recombinant inbred lines, derived from crossing sweet unsweet accessions, to narrow down size previously described content...
Abstract Background Cultivated watermelon [ Citrullus lanatus (Thunb.) Matsum. & Nakai var. ] is an important agriculture crop world-wide. The fruit of undergoes distinct stages development with dramatic changes in its size, color, sweetness, texture and aroma. In order to better understand the genetic molecular basis these significantly expand transcript catalog, we have selected four critical used Roche/454 next-generation sequencing technology generate a large expressed sequence tag...
Abstract Background Modern watermelon ( Citrullus lanatus L. ) cultivars share a narrow genetic base due to many years of selection for desirable horticultural qualities. Wild subspecies within C. are important potential sources novel alleles breeding, but successful trait introgression into elite has had limited success. The application marker assisted (MAS) in is yet be realized, mainly the past lack high quality maps. Recently, number useful maps have become available, however these few...
Years of selection for desirable fruit quality traits in dessert watermelon (Citrullus lanatus) has resulted a narrow genetic base modern cultivars. Development novel genomic and resources offers great potential to expand diversity improve important watermelon. Here, we report high-quality genome sequence cultivar 'Charleston Gray', principal American watermelon, complement the existing reference from '97103', an East Asian cultivar. Comparative analyses between genomes Gray' '97103'...
Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an important vegetable crop world-wide. fruit quality a complex trait determined by various factors such as sugar content, flesh color and texture. Fruit developmental process of cultivated wild watermelon are highly different. To systematically understand the molecular basis these differences, we compared transcriptome profiles tissues 97103 PI296341-FR. We identified 2,452, 826 322 differentially expressed genes in flesh, mesocarp...
Abstract There are three major dendritic cell (DC) subsets in both humans and mice, that is, plasmacytoid DCs two types of conventional (cDCs), cDC1s cDC2s. cDC2s important for polarizing CD4+ naive T cells into different subsets, including Th1, Th2, Th17, Th22, regulatory cells. In can be further divided phenotypically functionally distinct subgroups. However, human have not been reported. the present study, we showed blood CD1c+ cDCs (cDC2s) separated subpopulations according to their CD5...
Bottle gourd (Lagenaria siceraria) is an important vegetable crop as well a rootstock for other cucurbit crops. In this study, we report high-quality 313.4-Mb genome sequence of bottle inbred line, USVL1VR-Ls, with scaffold N50 8.7 Mb and the longest 19.0 Mb. About 98.3% assembled scaffolds are anchored to 11 pseudomolecules. Our comparative genomic analysis identifies chromosome-level syntenic relationships between cucurbits, lineage-specific gene family expansions in gourd. We...
Abstract Cucumber is vulnerable to many foliage diseases. Recent studies reported cloning of candidate genes for several diseases in cucumber; however, the exact defence mechanisms remain unclear. Dof have been shown play significant roles plant growth, development and responses biotic abiotic stresses. coding plant-specific transcription factors can promote large-scale expression defence-related at whole genome level. The family identified characterized species, but not cucumber. In present...
How raffinose (Raf) family oligosaccharides, the major translocated sugars in vascular bundle cucurbits, are hydrolyzed and subsequently partitioned has not been fully elucidated. By performing reciprocal grafting of watermelon (Citrullus lanatus) fruits to branch stems, we observed that Raf was fruit cultivar watermelons but backlogged wild ancestor species. Through a genome-wide association study, alkaline alpha-galactosidase ClAGA2 identified as key factor controlling stachyose...
Brassica oleracea includes several morphologically diverse, economically important vegetable crops, such as the cauliflower and cabbage. However, genetic variants, especially large structural variants (SVs), that underlie extreme morphological diversity of B. remain largely unexplored.Here we present high-quality chromosome-scale genome assemblies for two morphotypes, Direct comparison these identifies ~ 120 K high-confidence SVs. Population analysis 271 accessions using SVs clearly...
Abstract NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in fruit ripening quality. The watermelon genome encodes 80 genes, 21 of these genes are highly expressed both the flesh vascular tissues. Among ClNAC68 expression was significantly higher than rind. However, intrinsic regulatory mechanism quality is still unknown. In this study, we found that a transcriptional repressor repression domain located C-terminus. Knockout by CRISPR-Cas9 system decreased soluble solid...