A5056534103- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Advances in Cucurbitaceae Research
- Genomics and Phylogenetic Studies
- Plant Pathogens and Resistance
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Antioxidant Activity and Oxidative Stress
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Horticultural and Viticultural Research
- Nematode management and characterization studies
- Light effects on plants
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- RNA modifications and cancer
- RNA Research and Splicing
- Turfgrass Adaptation and Management
Huazhong Agricultural University
2016-2025
Guizhou University
2025
First People’s Hospital of Zunyi
2024
Zunyi Medical University
2024
Zhengzhou Fruit Research Institute
2020-2021
Chinese Academy of Agricultural Sciences
2020-2021
Kunming Institute of Zoology
2012
Chinese Academy of Sciences
2012
Plant Gene Expression Center
2005-2008
University of California, Berkeley
2005-2008
Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and central to global security. It clonally propagated, highly heterozygous, autotetraploid, suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone sequence assemble 86% of 844-megabase genome. We predict 39,031 protein-coding genes present evidence for at least two genome duplication events indicative palaeopolyploid origin. As first an asterid, reveals 2,642 specific this...
Abstract The Arabidopsis genome contains ∼200 genes that encode proteins with similarity to the nucleotide binding site and other domains characteristic of plant resistance proteins. Through a reiterative process sequence analysis reannotation, we identified 149 NBS-LRR–encoding in (ecotype Columbia) genomic sequence. Fifty-six these were corrected from earlier annotations. At least 12 are predicted be pseudogenes. As described previously, two distinct groups sequences identified: those...
Yijun Ruan and colleagues report the draft genome of sweet orange, Citrus sinensis. Their data suggests orange originated from a cross between pummelo mandarin. Oranges are an important nutritional source for human health have immense economic value. Here we present comprehensive analysis (Citrus sinensis). The assembled sequence covers 87.3% estimated genome, which is relatively compact, as 20% composed repetitive elements. We predicted 29,445 protein-coding genes, half in heterozygous...
Zhangjun Fei and colleagues report the draft genome of a Chinese elite watermelon inbred line 97103 resequencing 20 diverse accessions that represent three subspecies Citrullus lunatus. Comparative genome-wide analyses identify extent genetic diversity population structure germplasm. Watermelon, lanatus, is an important cucurbit crop grown throughout world. Here we high-quality sequence east Asia cultivar (2n = 2× 22) containing 23,440 predicted protein-coding genes. genomics analysis...
Sequencing and analysing the diploid genome transcriptome of Aegilops tauschii provide new insights into role this in enabling adaptation bread wheat are a step towards understanding very large complicated hexaploid genomes species. The Triticum aestivum, designated AABBDD, evolved as result hybridization between three ancestral grasses. Two papers published issue Nature present sequences analysis two these progenitors. First, sequence wild T. urartu (ancestor A genome), which resembles...
Late blight, caused by the oomycete pathogen Phytophthora infestans, is most devastating potato disease in world. Control of late blight United States and other developed countries relies extensively on fungicide application. We previously demonstrated that wild diploid species Solanum bulbocastanum highly resistant to all known races P. infestans. Potato germplasm derived from S. has shown durable effective resistance field. Here we report cloning major gene RB using a map-based approach...
Summary Comparative genomics provides a tool to utilize the exponentially increasing sequence information from model plants clone agronomically important genes less studied crop species. Plant disease resistance ( R ) loci frequently lack synteny between related species of cereals and crucifers but appear be positionally well conserved in Solanaceae. In this report, we adopted local RGA approach using genomic Solanaceous plant tomato isolate R3a , potato gene that confers race‐specific late...
High expression of plant nucleotide binding site leucine-rich repeat (NBS-LRR) defense genes is often lethal to cells, a phenotype perhaps associated with fitness costs. Plants implement several mechanisms control the transcript level NBS-LRR genes. As negative transcriptional regulators, diverse miRNAs target NBS-LRRs in eudicots and gymnosperms. To understand evolutionary benefits this miRNA-NBS-LRR regulatory system, we investigated 70 land plants, coupling analysis extensive small RNA...
Abstract Resistance Gene Candidate2 (RGC2) genes belong to a large, highly duplicated family of nucleotide binding site–leucine rich repeat (NBS-LRR) encoding disease resistance located at single locus in lettuce (Lactuca sativa). To investigate the genetic events occurring during evolution this locus, ∼1.5- 2-kb 3′ fragments 126 RGC2 from seven genotypes were sequenced three species Lactuca, and 107 additional sequences obtained 40 wild accessions Lactuca spp. The copy number varied 12 32...
Miniature inverted–repeat transposable elements (MITEs) are predicted to play important roles on genome evolution. We developed a BLASTN-based approach for de novo identification of MITEs and systematically analyzed in rice genome. The cultivar Nipponbare (Oryza sativa ssp. japonica) harbors 178,533 MITE-related sequences classified into 338 families. Pairwise nucleotide diversity phylogenetic tree analysis indicated that individual MITE families were resulted from one or multiple rounds...
Small RNAs regulate the genome by guiding transcriptional and post-transcriptional silencing machinery to specific target sequences, including genes transposable elements (TEs). Although miniature inverted-repeat (MITEs) are closely associated with euchromatic genes, broader functional impact of these short TE insertions in is largely unknown. We identified 22 families MITEs Solanaceae ( MiS1 – MiS22 ) found abundant MiS genomic DNA expressed sequence tags (EST). Several generate changes...
Miniature inverted-repeat transposable elements (MITEs) are prevalent in eukaryotic species including plants. MITE families vary dramatically and usually cannot be identified based on homology. In this study, we de novo MITEs from 41 plant species, using computer programs Digger, MITE-Hunter and/or Repetitive Sequence with Precise Boundaries (RSPB). were found all, but one (Cyanidioschyzon merolae), species. Combined the previously rice genome, >2.3 million sequences 3527 obtained general,...
Abiotic stresses are a major cause of crop loss. Ascorbic acid (AsA) promotes stress tolerance by scavenging reactive oxygen species (ROS), which accumulate when plants experience abiotic stress. Although the biosynthesis and metabolism AsA well established, genes that regulate these pathways remain largely unexplored. Here, we report on novel regulatory gene from tomato (Solanum lycopersicum) named SlZF3 encodes Cys2/His2-type zinc-finger protein with an EAR repression domain. The...
Abstract Betula L. (birch) is a pioneer hardwood tree species with ecological, economic, and evolutionary importance in the Northern Hemisphere. We sequenced platyphylla genome assembled sequences into 14 chromosomes. The lacks evidence of recent whole-genome duplication has same paleoploidy level as Vitis vinifera Prunus mume. Phylogenetic analysis lignin pathway genes coupled tissue-specific expression patterns provided clues for understanding formation higher ratios syringyl to guaiacyl...
Anthocyanins protect plants from biotic and abiotic stressors provide great health benefits to consumers. In this study, we cloned four genes (Red Lettuce Leaves 1 4: RLL1 RLL4) that contribute colour variations in lettuce. The gene encodes a bHLH transcription factor, 5-bp deletion some cultivars abolishes its function activate the anthocyanin biosynthesis pathway. RLL2 an R2R3-MYB which was derived duplication followed by mutations promoter region. RLL3 R2-MYB down-regulates through...
Massive resistance (R) gene stacking is considered to be one of the most promising approaches provide durable potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in an example natural R stacking, because it contains two closely linked genes (R3a R3b) with distinct specificities Phytophthora infestans. Here, we report about positional cloning R3b. Both transient stable transformations susceptible tobacco plants showed...
Abstract The proper use of resistance genes (R genes) requires a comprehensive understanding their genomics and evolution. We analyzed encoding nucleotide-binding sites leucine-rich repeats in the genomes rice (Oryza sativa), maize (Zea mays), sorghum (Sorghum bicolor), Brachypodium distachyon. Frequent deletions translocations R generated prevalent presence/absence polymorphism between different accessions/species. were caused by unequal crossover, homologous repair, nonhomologous or other...
Cultivated and wild potatoes contain a major disease-resistance cluster on the short arm of chromosome V, including R1 resistance (R) gene against potato late blight. To explore functional evolutionary significance clustering in generation novel genes, we constructed three approximately 1 Mb physical maps region, one for each genomes (haplotypes) allohexaploid Solanum demissum, progenitor locus. Totals 691, 919 559 kb were sequenced haplotype, distinct resistance-gene families identified,...
The sequenced genomes of cucumber, melon and watermelon have relatively few R-genes, with 70, 75 55 copies only, respectively. mechanism for low copy number R-genes in Cucurbitaceae remains unknown.Manual annotation the species showed that approximately half them are pseudogenes. Comparative analysis frequent loss R-gene loci different species. Phylogenetic analysis, data mining PCR cloning using degenerate primers indicated has limited lineages (subfamilies). Comparison between from those...
SUMMARY Folate (vitamin B9) is important for plant root development, but the mechanism largely unknown. Here we characterized a defective mutant, folb2 , in Arabidopsis, which has severe developmental defects primary root. The apical meristem of mutant impaired, and adventitious roots are frequently found at root–hypocotyl junction. Positional cloning revealed that 61‐bp deletion present predicted junction region promoter 5′ untranslated AtFolB2 gene encoding dihydroneopterin aldolase...
Abstract In experiments to detect heart disease on cardiac magnetic resonance imaging (MRI) medical images, existing object detection models face several challenges including low accuracy and unreliable results. To tackle these issues, this article proposes an innovative method for Object Detection in MRI images called SA-YOLO. This is based the YOLOv8 model but introduces key modifications. Firstly, standard Spatial Pyramid Pooling Fast module replaced with a Multi-Channel module. Secondly,...
<sec> <title>BACKGROUND</title> Online questionnaires are widely used for large-scale mental health screening. However, careless responses from participants can compromise data validity, ultimately undermining the reliability of screening outcomes. While prior studies have primarily focused on effects individual and environmental factors responding, role questionnaire type remains underexplored. Furthermore, few examined responding within contexts. </sec> <title>OBJECTIVE</title> This study...