A5100648679- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Plant Pathogenic Bacteria Studies
- Analytical Chemistry and Chromatography
- Plant Disease Resistance and Genetics
- Plant and Fungal Species Descriptions
- RNA regulation and disease
- Ionic liquids properties and applications
- Pulmonary Hypertension Research and Treatments
- Identification and Quantification in Food
- Circular RNAs in diseases
- Phytoplasmas and Hemiptera pathogens
- Fish biology, ecology, and behavior
- Autophagy in Disease and Therapy
- Botanical Research and Chemistry
- Genetic diversity and population structure
- Fish Biology and Ecology Studies
- MicroRNA in disease regulation
- RNA modifications and cancer
- Plant Virus Research Studies
- Research in Cotton Cultivation
- Ichthyology and Marine Biology
- Photosynthetic Processes and Mechanisms
Huazhong Agricultural University
2012-2025
Shanghai Chenshan Plant Science Research Center
2025
Center for Excellence in Molecular Plant Sciences
2025
Florida Museum of Natural History
2019-2024
University of Florida
2019-2024
Ningbo University
2024
Inner Mongolia University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2023
Chinese Academy of Sciences
2011-2023
Institute of Hematology & Blood Diseases Hospital
2022-2023
Yijun Ruan and colleagues report the draft genome of sweet orange, Citrus sinensis. Their data suggests orange originated from a cross between pummelo mandarin. Oranges are an important nutritional source for human health have immense economic value. Here we present comprehensive analysis (Citrus sinensis). The assembled sequence covers 87.3% estimated genome, which is relatively compact, as 20% composed repetitive elements. We predicted 29,445 protein-coding genes, half in heterozygous...
Abstract Gossypium hirsutum contributes the most production of cotton fibre, but G. barbadense is valued for its better comprehensive resistance and superior fibre properties. However, allotetraploid genome has not been comprehensively analysed. Here we present a high-quality assembly 2.57 gigabase , including 80,876 protein-coding genes. The double-sized A (or At) (1.50 Gb) against D Dt) (853 Mb) primarily resulted from expansion Gypsy elements, Peabody Retrosat2 subclades in Del clade...
Abstract Enhancer deregulation is a well-established pro-tumorigenic mechanism but whether it plays regulatory role in tumor immunity largely unknown. Here, we demonstrate that cell ablation of mixed-lineage leukemia 3 and 4 (MLL3 MLL4, also known as KMT2C KMT2D, respectively), two enhancer-associated histone H3 lysine (H3K4) mono-methyltransferases, increases immunogenicity promotes anti-tumor T response. Mechanistically, MLL4 attenuates the expression RNA-induced silencing complex (RISC)...
Artemisia subg. Seriphidium, one of the most species-diverse groups within Artemisia, grows mainly in arid or semi-arid regions temperate climates. Some members have considerable medicinal, ecological, and economic value. Previous studies on this subgenus been limited by a dearth genetic information inadequate sampling, hampering our understanding their phylogenetics evolutionary history. We therefore sequenced compared chloroplast genomes subgenus, evaluated phylogenetic relationships.We...
Citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all crops in world. Sweet orange accounts for more than half both fresh processed juice. We have sequenced draft genome a double-haploid sweet (C. sinensis cv. Valencia), constructed annotation project (CAP) to store visualize genomic transcriptome data. CAP provides GBrowse-based organization data, which integrates ab initio gene prediction, EST, RNA-seq RNA-paired end tag (RNA-PET)...
Ionic-liquids-based, microwave-assisted extraction (ILMAE) was successfully applied to the of two alkaloids: camptothecin (CPT) and 10-hydroxycamptothecin (HCPT) from samara Camptotheca acuminata. Ten different types 1-alkyl-3-methylimidazolium, with cations anions, were investigated in this work, 1-hexyl-3-methylimidazolium bromide ([C8MIM]Br) solution selected as optimal solvent. In addition, parameters, including ionic liquid concentration, soak time, microwave irradiation power,...
CaMKII has long been known to be a key effector for synaptic plasticity. Recent studies have shown that variety of modulators interact with the subunits regulate long-term potentiation (LTP) hippocampal neurons. However, whether non-coding RNAs modulate activity and affect plasticity is still elusive. Here, we identify previously uncharacterized RNA Carip functions as scaffold, specifically interacts CaMKIIβ, regulates phosphorylation α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid...
Phosphatidic acid (PA) functions as a cell membrane component and signaling molecule in plants. PA metabolism has multiple routes, one of which is converted into cytidine diphosphate diacylglycerol (CDP-DAG) by CDP-DAG synthases (CDSs). CDS genes are highly conserved Here, we found that knock-down the gene enhanced resistance Arabidopsis thaliana to pathogens, with growth penalty. When leaves were treated chitin or flg22, reactive oxygen species (ROS) production cds mutants was significantly...
Recently, the identification of several circular RNAs (circRNAs) as vital regulators microRNAs (miRNAs) underlines increasing complexity non-coding RNA (ncRNA)-mediated regulatory networks. This study aimed to explore effects mmu_circ_0000790 on biological behaviors pulmonary artery smooth muscle cells (PASMCs) in hypoxic hypertension (HPH). The HPH mouse model and hypoxia-induced PASMC were initially established, expression vascular tissues PASMCs was determined using quantitative reverse...
Next generation sequencing technologies have revolutionized our ability to rapidly and affordably generate vast quantities of sequence data. Once generated, raw sequences are assembled into contigs or scaffolds. However, these assemblies mostly fragmented inaccurate at the whole genome scale, largely due inability integrate additional informative datasets (e.g. physical, optical genetic maps). To address this problem, we developed a semi-automated software tool-Genome Puzzle Master...
Abstract ABC portal (http://abc.sklehabc.com) is a database and web containing 198 single-cell transcriptomic datasets of development, differentiation disorder blood/immune cells. All the were re-annotated with manually curated unified reference, especially for haematopoietic stem progenitor provides web-based interactive analysis modules, comprehensive cell-cell communication disease-related gene signature analysis. Importantly, allows customized sample selection based on combination...
The cyprinid subfamily Oxygastrinae is composed of a diverse group fishes that has been taxonomically and phylogenetically problematic. Their great variation in appearance, life histories, trophic diversity resulted uncertainty regarding their relationships, which led to historical classification across many disparate subfamilies. phylogenetic relationships are resolved based on sequence data from four loci: cytochrome b, c oxidase I, opsin, recombination activating gene 1. A combined matrix...
Hypoxia is a risk factor for severe chronic obstructive pulmonary disease, which aggravates the disease and may cause mortality by inducing hypoxic hypertension (HPH). Proliferation migration of artery smooth muscle cells (PASMCs) mediate this effect. Resveratrol phenolic compound extracted from plant has been reported to alleviate HPH, although underlying mechanisms remained be elucidated. In cancer, resveratrol abrogate phosphoinositide 3‑kinase/protein kinase B (AKT) signaling pathway,...
Agrobacterium tumefaciens strain C58 is a Gram-negative soil bacterium capable of inducing tumors (crown galls) on many dicotyledonous plants. The genome A. was re-annotated based the Z-curve method. First, all 'hypothetical genes' were re-identified, and 29 originally annotated recognized to be non-coding open reading frames (ORFs). Theoretical evidence obtained from principal component analysis, clusters orthologous groups proteins occupation, average length distribution showed that these...
This work was aimed at exploring the regulatory network of non-coding RNA (ncRNA) especially circular (circRNA) and microRNA (miRNA), in sensitivity non-small cell lung cancer (NSCLC) cells to low linear energy transfer (LET) X-ray high-LET carbon ion irradiations.The radioresistant NSCLC line A549-R11 obtained from its parental A549 through irradiation with X-rays 2.0 Gy per fraction for 30 times. The sensitivities A549, H1299 exposed ions were verified using colony formation assay. A...
An ionic liquids based simultaneous ultrasonic and microwave assisted extraction (ILs-UMAE) method has been proposed for the of rutin (RU), quercetin (QU), from velvetleaf leaves. The influential parameters ILs-UMAE were optimized by single factor central composite design (CCD) experiments. A 2.00 M 1-butyl-3-methylimidazolium bromide ([C4mim]Br) was used as experimental liquid, temperature 60°C, time 12 min, liquid-solid ratio 32 mL/g, power 534 W, a fixed 50 W. Compared to conventional...