A5005159202- Cancer Mechanisms and Therapy
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Epigenetics and DNA Methylation
- Asthma and respiratory diseases
- Mechanisms of cancer metastasis
- Reproductive System and Pregnancy
- Reproductive tract infections research
- Cancer Cells and Metastasis
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Circular RNAs in diseases
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Extracellular vesicles in disease
- Macrophage Migration Inhibitory Factor
- Phagocytosis and Immune Regulation
- interferon and immune responses
Tianjin Medical University
2015-2025
Sun Yat-sen University
2021-2025
Sun Yat-sen University Cancer Center
2025
Wuhan No.1 Hospital
2021-2025
Zhongnan Hospital of Wuhan University
2025
Wuhan University
2005-2025
Hubei University
2025
Jiangsu University
2021-2025
Ministry of Education of the People's Republic of China
2009-2024
Tianjin Medical University Cancer Institute and Hospital
2021-2024
Specific populations of highly tumorigenic cells are thought to exist in many human tumors, including pancreatic adenocarcinoma. However, the clinical significance these tumor-initiating (ie, cancer stem) remains unclear. Aldehyde dehydrogenase (ALDH) activity can identify and normal stem from several tissues. We examined prognostic functional features ALDH expression adenocarcinoma.ALDH was analyzed by immunohistochemistry 269 primary surgical specimens adenocarcinoma for association with...
There are currently two distinct models proposed to explain why both MDM2 and MDMX required in p53 control, with a key difference centered on whether these inhibitors work together or independently. To test competing models, we generated knockin mice expressing point mutation mutant (C462A) that is defective binding. This approach allowed targeted disassociation of the MDM2/MDMX heterocomplex without affecting ability bind p53, while leaving protein itself completely untouched....
Abstract Highly tumorigenic cancer cell (HTC) populations have been identified for a variety of solid tumors and assigned stem properties. Strategies identifying HTCs in primarily empirical rather than rational, particularly epithelial tumors, which are responsible 80% deaths. We report evidence spatially restricted bladder (urothelial) differentiation program primary urothelial cancers (UCs) UC xenografts. highly compartment that resembles benign cells (basal cells), co-expresses the 67-kDa...
Transcription and pre-mRNA splicing are the key nuclear processes in eukaryotic gene expression, identification of factors common to both has suggested that they functionally coordinated. p100 protein been shown function as a transcriptional co-activator for several transcription factors. consists staphylococcal nuclease (SN)-like Tudor-SN (TSN) domains which SN-like have transcription, but TSN domain remained elusive. Here we identified interaction between small ribonucleoproteins (snRNP)...
Abstract SND1 is an AEG-1/MTDH/LYRIC-binding protein that upregulated in numerous human cancers, where it has been assigned multiple functional roles. In this study, we report its association with the TGFβ1 signaling pathway, which promotes epithelial–mesenchymal transition (EMT) breast cancer. was cancer tissues, particular primary invasive ductal carcinomas. Transcriptional activation of gene controlled by TGFβ1/Smad specifically Smad2/Smad3 complex. The promoter region contained several...
Abstract Enhancer deregulation is a well-established pro-tumorigenic mechanism but whether it plays regulatory role in tumor immunity largely unknown. Here, we demonstrate that cell ablation of mixed-lineage leukemia 3 and 4 (MLL3 MLL4, also known as KMT2C KMT2D, respectively), two enhancer-associated histone H3 lysine (H3K4) mono-methyltransferases, increases immunogenicity promotes anti-tumor T response. Mechanistically, MLL4 attenuates the expression RNA-induced silencing complex (RISC)...
STAT6 is a critical regulator of transcription for interleukin-4 (IL-4)-induced genes. Activation gene expression involves recruitment coactivator proteins that function as bridging factors connecting sequence-specific to the basal machinery, and chromatin-modifying enzymes. Coactivator CBP/p300 have been implicated in regulation all STATs. CBP also required STAT6-mediated activation, but underlying molecular mechanisms are still elusive. In this study we investigated by which recruits...
Abstract Background Cancer stem cells (CSCs) are thought to be capable of surviving conventional chemotherapeutic treatments because the have more resistant anticancer drugs than common cancer cells. Most in vitro studies experimental been done a two-dimensional (2D) monocultures, while accumulating evidence suggests that behave differently when they grown within three-dimensional (3D) culture system. Results The CD44 + CD117 isolated from human epithelial ovarian SKOV-3 cell line using...
This study aims to explore the effects of exercise on postmenopausal osteoporosis and mechanisms by which affects bone remodeling. Sixty-three Wistar female rats were randomly divided into five groups: (1) control group, (2) sham-operated (3) OVX (Ovariectomy) (4) DES-OVX (Diethylstilbestrol-OVX) (5) Ex-OVX (Exercise-OVX) group. The rat model was established through ovariectomy. made run 251.2 meters every day, 6 d/wk for 3 months in a running wheel. Trabecular volume (TBV%), total...
The 5 year survival rate of lung cancer is <20%, with most patients dying from distant metastasis. However, the molecular mechanisms underlying invasion and metastasis have not been fully characterized. In this study, we found that fibulin-3, a fibulin family extracellular matrix protein, functions as suppressor Fibulin-3 was downregulated in large fractions tumors cell lines, inhibited expression metalloproteinase-7 (MMP-7), promoter invasion. levels fibulin-3 MMP-7 were inversely...
STAT6 participates in classical IL-4/IL-13 signaling and stimulator of interferon genes-mediated antiviral innate immune responses. Aberrations STAT6-mediated are linked to development asthma diseases the system. In addition, remains constitutively active multiple types cancer. Therefore, targeting is an attractive proposition for treating related diseases. Although a lot known about role transcriptional regulation, molecular details on how recognizes binds specific segments DNA exert its...
Tudor staphylococcal nuclease (Tudor-SN) is a multifunctional protein implicated in variety of cellular processes. In the present study, we identified Tudor-SN as novel regulator cell cycle. was abundant proliferating cells whereas barely expressed terminally differentiated cells. Functional analysis indicated that ectopic overexpression promoted G1/S transition, knockdown caused G1 arrest. Moreover, live-cell time-lapse experiment demonstrated cycle MEF(-/-) (knock-out mouse embryonic...
Background: Radiotherapy is one of the most effective therapeutic strategies for cervical cancer patients, although radioresistance-mediated residual and recurrent tumors are main cause treatment failure. However, mechanism tumor radioresistance still elusive. DNA damage response pathways key determinants radioresistance. The purpose this study was to investigate role SND1 in cancer. Methods: A stable HeLa cell line with knockout (HeLa-KO) generated through a modified CRISPR/Cas9...
The RNA N6-methyladenosine (m6A) modification is a critical regulator of various biological processes, but precise and dynamic control m6A remains challenge. In this work, we present red/far-red light-inducible editing system that enables efficient reversible modulation levels with minimal off-target effects. By engineering the CRISPR dCas13 protein sgRNA two pairs heterodimerizing proteins, ΔphyA/FHY1 Bphp1/PspR2, achieved targeted recruitment effectors. This significantly enhances writing...
Several liver diseases have been associated with the Tudor staphylococcal nuclease (Tudor‐SN) protein. Our previous results demonstrated that, in comparison to wild‐type (WT) mice, systemic overexpression of Tudor‐SN transgenic (Tg) mice (Tudor‐SN‐Tg) ameliorates obesity‐induced insulin resistance and hepatic steatosis. In this study, we observed an inverse correlation expression levels profibrogenic factors, such as alpha‐smooth muscle actin (α‐SMA) collagen alpha‐1(I) chain (COL1A1),...
Signal transducer and activator of transcription 5 (Stat5) plays a critical role in prolactin (PRL)-induced several milk protein genes. Stat5-mediated gene regulation is modulated by cooperation Stat5 with cell type- promoter-specific factors as well interaction transcriptional coregulators. Recently, the expression tudor staphylococcal nuclease-like domains containing p100 was found to be increased mammary epithelial cells during lactation response lactogenic hormones. initially identified...