A5100351902- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- Cell death mechanisms and regulation
- Molecular Biology Techniques and Applications
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Cancer Mechanisms and Therapy
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- RNA Research and Splicing
- Lung Cancer Treatments and Mutations
- Circular RNAs in diseases
- PARP inhibition in cancer therapy
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Treatments and Studies
- PI3K/AKT/mTOR signaling in cancer
- Cancer Genomics and Diagnostics
- Peptidase Inhibition and Analysis
- Forensic and Genetic Research
- Cytokine Signaling Pathways and Interactions
- RNA Interference and Gene Delivery
Jining Traditional Chinese Medicine Hospital
2025
University of Southern California
2006-2025
Wuxi People's Hospital
2024-2025
Nanjing Medical University
2010-2025
Sichuan University
2015-2025
Henan Agricultural University
2024-2025
Affiliated Hospital of Hangzhou Normal University
2025
Zhongnan Hospital of Wuhan University
2018-2025
Wuhan University
2018-2025
Fourth Affiliated Hospital of Harbin Medical University
2016-2025
The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis gene expression (SAGE), that allows quantitative and simultaneous a large number transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, cloned. Manual sequencing 1000 revealed pattern characteristic pancreatic function. New transcripts corresponding to novel identified. SAGE should provide broadly...
Exposure of colorectal cancer (CRC) cells to ionizing radiation results in a cell-cycle arrest G1 and G2. The is due p53-mediated induction the cyclin-dependent kinase inhibitor p21WAF1/CIP1/SDI1, but basis for G2 unknown. Through quantitative analysis gene expression patterns CRC cell lines, we have discovered that 14-3-3sigma strongly induced by gamma irradiation other DNA-damaging agents. mediated p53-responsive element located 1.8 kb upstream its transcription start site. Exogenous...
As a step toward understanding the complex differences between normal and cancer cells in humans, gene expression patterns were examined gastrointestinal tumors. More than 300,000 transcripts derived from at least 45,000 different genes analyzed. Although extensive similarity was noted profiles, more 500 that expressed significantly levels neoplastic identified. These data provide insight into extent of underlying malignancy reveal may prove useful as diagnostic or prognostic markers.
We have analyzed the set of genes expressed from yeast genome, herein called transcriptome, using serial analysis gene expression. Analysis 60,633 transcripts revealed 4,665 genes, with expression levels ranging 0.3 to over 200 per cell. Of these 1981 had known functions, while 2684 were previously uncharacterized. The integration positional information data allowed for generation chromosomal maps identifying physical regions transcriptional activity and identified that not been predicted by...
We have developed an in vitro method for amplifying a large fraction of the DNA sequences present single haploid cell by repeated primer extensions using mixture 15-base random oligonucleotides. studied 12 genetic loci and estimate that probability any sequence genome to minimum 30 copies is not less than 0.78 (95% confidence). Whole amplification beginning with cell, or other samples very small amounts DNA, has significant implications multipoint mapping sperm oocyte typing possibly disease...
To assess the role of BAX in drug-induced apoptosis human colorectal cancer cells, we generated cells that lack functional genes. Such were partially resistant to apoptotic effects chemotherapeutic agent 5-fluorouracil, but was not abolished. In contrast, absence completely abolished response chemopreventive sulindac and other nonsteroidal anti-inflammatory drugs (NSAIDs). NSAIDs inhibited expression antiapoptotic protein Bcl-X L , resulting an altered ratio subsequent mitochondria-mediated...
Ferroptosis is a form of regulated cell death that may facilitate the selective elimination tumor cells. The suppressor p53 (TP53) has been demonstrated to promote ferroptosis via transcription-dependent mechanism. Here, we show TP53 limits erastin-induced by blocking dipeptidyl-peptidase-4 (DPP4) activity in transcription-independent manner. Loss prevents nuclear accumulation DPP4 and thus facilitates plasma-membrane-associated DPP4-dependent lipid peroxidation, which finally results...
Although several genes that might mediate p53-induced apoptosis have been proposed, none previously shown to play an essential role in this process through a rigorous gene disruption approach. We used gene-targeting approach evaluate p53-mediated death human colorectal cancer cells. Expression of p53 these cells induces growth arrest transcriptional activation the cyclin-dependent kinase inhibitor p21 . If is disrupted via targeting, die apoptosis. PUMA also such cells, prevented. The...
Using gene targeting in embryonic stem cells, we have derived mice with a null mutation DNA mismatch repair homolog, PMS2. We observed microsatellite instability the male germline, tail, and tumor of PMS2-deficient animals. therefore conclude that PMS2 is involved variety tissues. animals appear prone to sarcomas lymphomas. males are infertile, producing only abnormal spermatozoa. Analysis axial element synaptonemal complex formation during prophase meiosis I indicates abnormalities...
Sequence-specific transactivation by p53 is essential to its role as a tumor suppressor. A modified tetracycline-inducible system was established search for transcripts that were activated soon after induction. Among 9,954 unique identified serial analysis of gene expression, 34 increased more than 10-fold; 31 these had not previously been known be regulated p53. The transcription patterns genes, well described p53-regulated evaluated and classified in panel widely studied colorectal cancer...
Gene knockout is the most powerful tool for determining gene function or permanently modifying phenotypic characteristics of a cell. Existing methods disruption are limited by their efficiency, time to completion, and/or potential confounding off-target effects. Here, we demonstrate rapid single-step approach targeted in mammalian cells, using engineered zinc-finger nucleases (ZFNs). ZFNs can be designed target chosen locus with high specificity. Upon transient expression these first cleaved...
Abstract microRNAs (miRNA) are small noncoding RNAs that participate in diverse biological processes by suppressing target gene expression. Altered expression of miR-21 has been reported cancer. To gain insights into its potential role tumorigenesis, we generated knockout colon cancer cells through targeting. Unbiased microarray analysis combined with bioinformatics identified cell cycle regulator Cdc25A as a target. suppressed defined sequence 3′-untranslated region. We found is induced...
Abstract Background High invasion and metastasis are the primary factors causing poor prognosis of patients with hepatocellular carcinoma (HCC). However, molecular mechanisms underlying these biological behaviors have not been completely elucidated. In this study, we investigate mechanism by which hypoxia promotes HCC through inducing epithelial-mesenchymal transition (EMT). Methods The expression EMT markers was analyzed immunohistochemistry. Effect on induction ability cell migration were...
Abstract The balance between apoptosis (“programmed cell death”) and autophagy survival”) is important in tumor development response to therapy. Here, we show that high mobility group box 1 (HMGB1) p53 form a complex regulates the death survival. We knockout of HCT116 cells increases expression cytosolic HMGB1 induces autophagy. Conversely, mouse embryonic fibroblasts localization decreases thus negative regulator HMGB1/Beclin complex, promotes setting diminished p53. HMGB1-mediated survival...
We simulate and analyze the mode properties propagation effects of orbital angular momentum (OAM) modes in a ring fiber. A fiber with 0.05 up-doping is designed simulation to support up 10 OAM while maintaining single-mode condition radially. With multiple-ring fiber, tens can be potentially multiplexed greatly enhance system capacity spectral efficiency. The index difference maintained above <sup xmlns:mml="http://www.w3.org/1998/Math/MathML"...