A5101744500- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Genomics and Rare Diseases
- PARP inhibition in cancer therapy
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Connective tissue disorders research
- Traditional and Medicinal Uses of Annonaceae
- Lipid metabolism and disorders
- Dermatological and Skeletal Disorders
- Diet and metabolism studies
- Hemoglobinopathies and Related Disorders
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- DNA and Nucleic Acid Chemistry
- Frailty in Older Adults
- Berberine and alkaloids research
- Central Venous Catheters and Hemodialysis
- Wnt/β-catenin signaling in development and cancer
- Traditional Chinese Medicine Studies
- Alkaloids: synthesis and pharmacology
Tianjin Medical University Cancer Institute and Hospital
2021-2024
First Affiliated Hospital of Zhengzhou University
2024
Shanghai Jiao Tong University
2016-2024
Nanchong Central Hospital
2024
Shanghai Ninth People's Hospital
2024
Tianjin Medical University
2019-2023
Shanghai Clinical Research Center
2021-2022
Tianjin Medical University General Hospital
2021
Shanghai Sixth People's Hospital
2021
Nanyang Institute of Technology
2021
The impaired differentiation ability of resident cells and disordered immune microenvironment in periodontitis pose a huge challenge for bone regeneration. Herein, we construct piezoelectric hydrogel to rescue the osteogenic capability rebuild regenerative through bioenergetic activation. Under local mechanical stress, generated piezopotential that initiates inflammatory periodontal ligament stem (PDLSCs) via modulating energy metabolism promoting adenosine triphosphate (ATP) synthesis....
Epigenetic regulation has emerged to be the critical steps for tumorigenesis and metastasis. Multiple histone methyltransferase demethylase have been implicated as tumor suppressors or oncogenes recently. But key epigenomic events in cancer cell transformation still remain poorly understood. A breast model was established via stably expressing three primary epithelial cells. Chromatin immunoprecipitation followed by next-generation sequencing of methylations performed determine epigenetic...
PHF8-promoted non-histone methylation switch plays a critical role in replication stress response and genome integrity.
MicroRNAs (miRNAs) exhibit aberrant expression in the initiation and progression of a variety human cancers, including colorectal cancer (CRC). However, exact mechanisms are not well defined. miRNA profiles were characterized by microarrays CRC samples, 18b (miR-18b) was increased significantly tumor tissues. The miR-18b confirmed cell lines SW480 HCT116 44 clinical specimens quantitative real-time PCR (qRT-PCR). Multiple linear regression analysis showed strong correlation with lymph node...
Rationale: A number of guanine nucleotide exchange factors (GEFs) including epithelial cell transforming factor ECT2 are believed to drive carcinogenesis through activating distinct oncogenic GTPases. Yet, whether GEF-independent activity also plays a role in tumorigenesis remains unclear. Methods: Immunohistochemical (IHC) staining, colony formation and xenograft assays were used examine the breast carcinogenesis. Co-immunoprecipitation, immunofluorescent stainings, vivo deubiquitination...
Genetic skeletal dysplasias (GSDs) are a type of disease with complex phenotype and high heterogeneity, characterized by cartilage bone growth abnormalities. The variable phenotypes GSD make clinical diagnosis difficult. To explore the utility targeted exome sequencing (TES) in GSD, 223 probands suspected were enrolled for TES panel 322 known disease-causing genes. After bioinformatics analysis, all candidate variants prioritized pathogenicity. Sanger was used to verify parents trace source...
Iron is essential for rapidly dividing spermatocytes during normal mammalian spermatogenesis. Decreased transferrin and receptor levels were observed in seminal plasma from idiopathic azoospermia (IA) patients, suggesting disturbed iron metabolism IA testes. However, how Sertoli cells (SCs) contribute to the homoeostasis still unclear. In this study, we analysed 30 age-matched obstructive (OA) patients undergoing testicular sperm aspiration (TESA). SCs hyperplasia was indicated by higher SC...
Spondylo-epi-metaphyseal dysplasia (SEMD) is a heterogeneous group of disorders with different modes inheritance and characterized by disproportionate or proportionate short stature. To date, more than 30 disease-causing genes have been identified, types SEMD exhibit greatly overlapping clinical features, which usually complicate the diagnosis. This study was performed to expand molecular spectrum among Chinese subjects explore their potential phenotype–genotype relations. We enrolled seven...
During the onset of nonalcoholic fatty liver disease (NAFLD), original deficiency is root cause its occurrence, and actual condition basic for occurrence. Therefore, correlation between law NAFL syndrome formation, pathogenesis, development, change. Based on this, purpose this article to study analyze intervention NAFLD from perspective true standard. In article, TCM physical fitness determination related risk factors investigation were conducted qualified patients collected outpatient...
Appropriate DNA end synapsis, regulated by core components of the synaptic complex including KU70-KU80, LIG4, XRCC4, and XLF, is central to non-homologous joining (NHEJ) repair chromatinized double-strand breaks (DSBs). However, it remains enigmatic whether chromatin modifications can influence formation NHEJ at ends, if so, how this achieved. Here, we report that mitotic deacetylase (MiDAC), serves as a key regulator synapsis during repair. Mechanistically, MiDAC removes combinatorial...
Abstract Although it is well established that the epithelial-mesenchymal transition (EMT) generates cells with properties of stem cells, molecularmechanisms responsible for this change remain largely undefined. We set out to identify mechanisms by examining expression cell markers as microRNAs in MCF-10A during EMT induction. By immunostaining and FACS analysis, we found induction either TGF-β treatment or overexpression KLF8, both CD44high/CD24low activity aldehyde dehydrogenase (ALDH) were...
The checkpoint kinase ATR is a master regulator of DNA damage response. Yet, how activity regulated remains to be investigated. We report here that histone demethylase PHF8 critically required for activation and cellular response ATR-activating lesions. With combined approach structural biochemical analysis, we unveiled an extraordinary feature the association/dissociation with TOPBP1, essential allosteric activator ATR. removes TOPBP1 K118 mono-methylation facilitate association RAD9, thus...
Adefovir dipivoxil (ADV) is widely used for chronic hepatitis B therapy in China. To explore the clinical features and prognosis of ADV-induced osteomalacia to analyze association between genetic variants 51 drug transporters genes. Clinical follow-up data ADV-treated patients were collected. Target capture sequencing was identify variations transporter A total 193 treated with ADV enrolled, whom 140 had osteomalacia. The other 53 without included control group. median duration treatment...
Homologous recombination (HR) is a high-fidelity, template-dependent repair pathway of DNA double-strand breaks (DSBs). The efficiency HR system tightly associated with tumor response to chemotherapy. Yet, how activity regulated remains be investigated. Here, we report that the RNA binding protein HTATSF1 physically Topoisomerase II-binding 1 (TOPBP1) in cell-cycle and phosphorylation dependent manner. In specific, S-phase enriched phosphorylated by CK2 kinase at Ser748 mark read N-terminal...