A5035939771- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- COVID-19 Clinical Research Studies
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Long-Term Effects of COVID-19
- Genomics and Chromatin Dynamics
- Respiratory Support and Mechanisms
- Cancer Mechanisms and Therapy
- Chronic Obstructive Pulmonary Disease (COPD) Research
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Air Quality and Health Impacts
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Energy, Environment, Economic Growth
- Peptidase Inhibition and Analysis
- Energy Efficient Wireless Sensor Networks
- Environmental Impact and Sustainability
- Management of metastatic bone disease
- Sepsis Diagnosis and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Congenital Diaphragmatic Hernia Studies
Zhongshan Hospital
2020-2025
Fudan University
2020-2025
Shanghai Jiao Tong University
2021-2025
Sun Yat-sen University
2021-2024
The First Affiliated Hospital, Sun Yat-sen University
2021-2024
Shanghai Institute of Hematology
2024
National Institute for Communicable Disease Control and Prevention
2024
National Institute for Viral Disease Control and Prevention
2023
KU Leuven
2018-2021
Wuhan No.9 Hospital
2020-2021
<h3>Importance</h3> Coronavirus disease 2019 (COVID-19) is an emerging infectious that was first reported in Wuhan, China, and has subsequently spread worldwide. Risk factors for the clinical outcomes of COVID-19 pneumonia have not yet been well delineated. <h3>Objective</h3> To describe characteristics patients with who developed acute respiratory distress syndrome (ARDS) or died. <h3>Design, Setting, Participants</h3> Retrospective cohort study 201 confirmed admitted to Wuhan Jinyintan...
Abstract Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of embryonic origin offer potential to generate patient-specific cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, iPSCs (hiPSCs), restricting their post-implantation development modeling. Hence, there is a need for hiPSCs resembling preimplantation naive epiblast. Here, we...
Background. Particulate matter (PM) exposure is related to mitochondria dysfunction and airway inflammation. Antioxidant drug edaravone (EDA) reported improve the occurrence development of oxidative stress-related diseases. At present, there no data on whether EDA can alleviate lung inflammation caused by PM. Methods. The anti-inflammatory effects were investigated in urban PM-induced human bronchial epithelial cells (HBECs) C57/BL6J mouse models. In vitro, its production intracellular...
Tudor staphylococcal nuclease (Tudor-SN) is a multifunctional protein implicated in variety of cellular processes. In the present study, we identified Tudor-SN as novel regulator cell cycle. was abundant proliferating cells whereas barely expressed terminally differentiated cells. Functional analysis indicated that ectopic overexpression promoted G1/S transition, knockdown caused G1 arrest. Moreover, live-cell time-lapse experiment demonstrated cycle MEF(-/-) (knock-out mouse embryonic...
Airway mucus hypersecretion contributes to the morbidity and mortality in patients with chronic inflammatory lung diseases. Reducing production is crucial for improving patients' quality of life. The transcription factor SAM-pointed domain-containing Ets-like (SPDEF) plays a critical role regulation and, therefore, represents potential therapeutic target. This study aims reduce epithelial by targeted silencing SPDEF using novel strategy, epigenetic editing. Zinc fingers CRISPR/dCas platforms...
Abstract Background The impact of corticosteroid therapy on outcomes patients with coronavirus disease 2019 (COVID-19) is highly controversial. We aimed to compare the risk death between COVID-19-related ARDS treatment and those without. Methods In this single-center retrospective observational study, caused by COVID-19 January 20, 2020, February 24, were enrolled. primary outcome was 60-day in-hospital death. exposure prescribed systemic corticosteroids or not. Time-dependent Cox regression...
Induction and reversal of chromatin silencing is critical for successful development, tissue homeostasis, the derivation induced pluripotent stem cells (iPSCs). X-Chromosome inactivation (XCI) reactivation (XCR) in female represent chromosome-wide transitions between active inactive states. Although XCI has long been studied, providing important insights into gene regulation, dynamics mechanisms underlying stable X-linked genes are much less understood. Here, we use allele-specific...
Particulate matter (PM) has become the main risk factor for public health, being linked with an increased of respiratory diseases. However, potential mechanisms underlying PM-induced lung injury have not been well elucidated. In this study, we systematically integrated metabolomics, lipidomics, and transcriptomics data obtained from human bronchial epithelial cells (HBECs) exposed to PM reveal metabolic disorders in injury. We identified 170 differentially expressed metabolites (82...
A dramatic difference in global DNA methylation between male and female cells characterizes mouse embryonic stem (ESCs), unlike somatic cells. We analyzed changes during reprogramming of resulting induced pluripotent (iPSCs). At an intermediate stage, pluripotency enhancers are targeted for partial demethylation. Demethylation within often occurs at ESC binding sites transcription factors. Late reprogramming, hypomethylation is a female-specific manner. Genome-wide affects many genomic...
Goblet cell metaplasia, a common feature of chronic obstructive pulmonary disease (COPD), is associated with mucus hypersecretion which contributes to the morbidity and mortality among patients. Transcription factors SAM-pointed domain-containing Ets-like factor (SPDEF) forkhead box protein A2 (FOXA2) regulate goblet differentiation. This study aimed (1) investigate DNA methylation expression SPDEF FOXA2 during differentiation (2) compare this in airway epithelial cells from patients COPD...
Stress granules (SGs) and processing bodies (PBs) comprise the main types of cytoplasmic RNA foci during stress. Our previous data indicate that knockdown human Tudor staphylococcal nuclease (Tudor-SN) affects aggregation SGs. However, precise molecular mechanism has not been determined fully. In present study, we demonstrate Tudor-SN binds colocalizes with many core components SGs, such as poly(A)(+) mRNA binding protein 1, T-cell internal antigen-1-related mRNA, SG/PB sharing proteins...
Highlights•X-chromosome dosage modulates the pluripotent chromatin accessibility landscape•Increased X-chromosome slows down growth•Dusp9 heterozygous female ESCs display pluripotency exit delaySummaryReprogramming mouse somatic cells into induced stem (iPSCs) leads to reactivation. The extent which increased (X-dosage) in iPSCs compared with male differences properties of is still unclear. We show that modulated by X-dosage. Specific sets transcriptional regulator motifs are enriched XX or...
Smoking-related lung tumors are characterized by profound epigenetic changes including scrambled patterns of DNA methylation, deregulated histone acetylation, altered gene expression levels, distorted microRNA profiles, and a global loss cytosine hydroxymethylation marks. Here, we employed an enhanced version bisulfite sequencing (RRBS/oxRRBS) followed next generation to separately map marks 5-methyl-dC 5-hydroxymethyl-dC in genomic isolated from lungs A/J mice exposed whole-body...
Glucocorticoid (GC) insensitivity is an important feature of severe and fatal asthma. Oxidative stress can induce phosphoinositide-3-kinase (PI3K) activation, contributing to the development GC in chronic airway diseases. However, underlying molecular mechanism PI3K pathogenesis asthma remains unknown.We isolated peripheral blood mononuclear cells (PBMCs) from 34 participants (12 patients with mild/moderate asthma, 10 12 control subjects). H2O2 was used stimulate human macrophage line U937...
Abstract Background Precise gene dosage of the X chromosomes is critical for normal development and cellular function. In mice, XX female somatic cells show transcriptional chromosome upregulation their single active chromosome, while other inactive. Moreover, inactive reactivated during in inner cell mass germ through reactivation, which can be studied vitro by reprogramming to pluripotency. How chromatin processes regulatory networks evolved regulate state reactivation remains unclear....
Introduction To investigate the risk factors for death in patients with COVID-19 type 2 diabetes mellitus (T2DM). Research design and methods We retrospectively enrolled inpatients from Wuhan Jinyintan Hospital (Wuhan, China) between December 25, 2019, March 3, 2020. The epidemiological clinical data were compared non-T2DM T2DM or survivors non-survivors. Univariable multivariable Cox regression analyses used to explore effect of complications on in-hospital death. Results A total 1105...
Abstract Introduction COVID‐19 has spread rapidly worldwide and been declared a pandemic. Objectives To delineate clinical features of patients with different severities prognoses clarify the risk factors for disease progression death at an early stage. Methods Medical history, laboratory findings, treatment outcome data from 214 hospitalised pneumonia admitted to Eastern Campus Renmin Hospital, Wuhan University in China were collected 30 January 2020 20 February 2020, associated...
ABSTRACT Staphylococcal nuclease domain containing 1 (SND1) is a ubiquitously expressed multifunctional protein involved in transcriptional regulation, RNA splicing and metabolism. Ectopic expression of SND1 has been observed various tumors including colon cancer, breast prostate cancer hepatocellular carcinoma (HCC), indicating positive role tumor initiation progression. However, the exact cancers not thoroughly investigated. In present study, we investigated HCC. Immunohistochemistry...