A5065399500- Diatoms and Algae Research
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Marine Biology and Ecology Research
- Marine and coastal plant biology
- Marine Toxins and Detection Methods
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Paleontology and Stratigraphy of Fossils
- Neonatal Respiratory Health Research
- Aquatic Invertebrate Ecology and Behavior
- Lichen and fungal ecology
- Genetic diversity and population structure
- Gene expression and cancer classification
- Parasite Biology and Host Interactions
- Methane Hydrates and Related Phenomena
- Echinoderm biology and ecology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Identification and Quantification in Food
- Coral and Marine Ecosystems Studies
- Water Quality Monitoring and Analysis
- Epigenetics and DNA Methylation
- Mollusks and Parasites Studies
- Wastewater Treatment and Nitrogen Removal
Stazione Zoologica Anton Dohrn
2015-2025
University of Groningen
1986-2023
University Medical Center Groningen
2017-2023
University of Naples Federico II
2018
Smithsonian Tropical Research Institute
1998-2005
Smithsonian Institution
1999
Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung
1996-1997
The interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack taxonomically curated reference data sets for targeted genes. Protist Ribosomal Reference database (PR2, http://ssu-rrna.org/) provides a unique access to eukaryotic small sub-unit (SSU) ribosomal RNA and DNA sequences, with taxonomy. mainly consists nuclear-encoded protistan sequences. However, metazoans, land plants, macrosporic fungi organelles (mitochondrion, plastid...
The morphology of strains Skeletonema Greville emend Sarno et Zingone was examined in LM, TEM, and SEM compared with sequence data from nuclear small subunit rDNA partial large rDNA. Eight distinct entities were identified, which four known: S. menzelii Guillard, Carpenter Reimann; pseudocostatum Medlin emend. Sarno; subsalsum (Cleve) Bethge; tropicum Cleve. other species new: dohrnii Kooistra sp. nov., grethae japonicum marinoi nov. fell into morphologically groups corresponding to lineages...
Although protists are critical components of marine ecosystems, they still poorly characterized. Here we analysed the taxonomic diversity planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) ribonucleic (RNA) from three size fractions (pico-, nano- micro/mesoplankton), as well dissolved DNA surface sediments were used templates for tag pyrosequencing V4 region 18S ribosomal DNA. Beta-diversity analyses split...
We tracked temporal changes in protist diversity at the Long Term Ecological Research (LTER) station MareChiara Gulf of Naples (Mediterranean Sea) on eight dates 2011 using a metabarcoding approach. Illumina analysis V4 and V9 fragments 18S rDNA produced 869 522 1 410 071 sequences resulting 6517 6519 OTUs, respectively. Marked compositional variations were recorded across year, with less than 2% OTUs shared among all samples similar patterns for two marker tags. Alveolata, Stramenopiles...
The genus Caulerpa consists of about 75 species tropical to subtropical siphonous green algae. To better understand the evolutionary history genus, a molecular phylogeny was inferred from chloroplast tuf A sequences 23 taxa. sequence Caulerpella ambigua included as potential outgroup. Results reveal that latter taxon is, indeed, sister all ingroup sequences. itself series relatively ancient and species‐poor lineages modern rapidly diversifying clade, containing most diversity. conflicts with...
Skeletonema comprises a planktonic genus of centric diatoms occurring in coastal and brackish waters worldwide. The most commonly reported species, costatum, was believed to be ubiquitous, morphologically plastic euryhaline. Yet, recent studies showed that this perceived taxon consists series including more narrowly defined costatum sensu stricto. Here we investigated the effect salinity on growth rate cell morphology. Ten strains belonging six species were incubated at salinities between 0...
Marine protist diversity inventories have largely focused on planktonic environments, while benthic protists received relatively little attention. We therefore hypothesize that current surveys only skimmed the surface of in marine sediments, which may harbor greater than environments. tested this by analyzing sequences hypervariable V4 18S rRNA from and communities sampled European coastal regions. Despite a similar number OTUs both realms, richness estimations indicated we recovered at...
Scrippsiella trochoidea is a widely distributed neritic dinoflagellate that produces calcareous resting cysts. We assessed the level of intraspecific diversity at molecular, morphological and physiological levels among 15 strains identified as S. isolated from Gulf Naples (Italy, Mediterranean Sea), an additional isolate Faeroe Islands. investigated morphology motile cells cysts, mating modality, encystment success, growth rates different light irradiances. The ribosomal DNA internal...
ABSTRACT. The establishment of chloroplasts as cellular organelles in the dinoflagellate, heterokont (stramenopile), haptophyte, and cryptophyte algae is widely accepted to have been result secondary endosymbiotic events, that is, uptake a photosynthetic eukaryote by phagotrophic eukaryote. However, circumstances promote such associations between two phylogenetically distinct organisms integration their genomes form single functional cell unclear. dinoflagellates Peridinium foliaceum...
Skeletonema costatum (Grev.) Cleve emend. Zingone et Sarno and S. grevillei were known only from the type material collected Hong Kong waters more than a century ago. Both species have now been as live material, their morphology phylogenetic position are investigated in this study. Eight strains isolated Florida, USA; Uruguay; Brazil attributed to , while one strain Oman is ascribed based on morphological similarity of these species. In addition, new species, ardens Zingone, described for...
Abstract The rhodophyte seaweed Asparagopsis armata Harvey is distributed in the northern and southern temperate zones, its congener taxiformis (Delile) Trevisan abounds throughout tropics subtropics. Here, we determine intraspecific phylogeographic patterns to compare potential causes of disjunctions distributions both species. We obtained specimens their ranges inferred phylogenies from hypervariable domains D1‐D3 nuclear rDNA LSU, plastid spacer between large small subunits RuBisCo...
ABSTRACT Aim Because of their broad distribution in geographical and ecological dimensions, seaweeds (marine macroalgae) offer great potential as models for marine biogeographical inquiry exploration the interface between macroecology macroevolution. This study aims to characterize evolutionary niche dynamics common green seaweed genus Halimeda , use observed insights gain understanding history predict habitats that can be targeted discovery species special interest. Location Tropical...
Abstract Marine protists have traditionally been assumed to be lowly diverse and cosmopolitan. Yet, several recent studies shown that many protist species actually consist of cryptic complexes whose members are often restricted particular biogeographic regions. Nonetheless, detection is usually hampered by sampling coverage application methods (e.g. phylogenetic trees) not well suited identify relatively divergence ongoing gene flow. In this paper, we show how these issues can overcome...
Abstract Diatoms’ bloom dynamics, with seasonal cycling of waxes and wanes cell abundance, imply frequent bottleneck expansion events that can leave signatures in the genetic structure diversity populations. We explored changes within multiple species planktonic marine diatom genus Pseudo ‐ nitzschia living sympatry Gulf Naples (Tyrrhenian Sea, Italy) over 48 sampling dates (from 2011 to 2013) by means metabarcode data. The includes complexes cryptic along morphologically distinguishable...
A phylogeny of the diatoms was inferred from comparisons nuclear-encoded small subunit ribosomal RNA coding regions using maximum likelihood, weighted parsimony, and neighbor-joining distance methods with Jukes Cantor, Kimura, Gamma, van de Peer, LogDet evolutionary models. Analyses 30 taxa in 11 orders recovered two clades (Clades I II). Neither these correspond to three classes presently recognized or traditionally radially symmetrical centric bilaterally pennate diatoms. All analyses show...
ABSTRACT Nucleotides were compared at 988 sites, spanning both internal transcribed spacers (ITS1 and ITS2) of the nuclear ribosomal DNA, among 17 isolates green alga Cladophoropsis membranacea (Hofman Bang ex C. Agardh) Boergesen two Struvea anastomosans (Harvey) Piccone Grunow. Collections made from Bonaire, Curaçao, St. Croix, Canary Islands, Cape Verde Mauritania, Syria, Red Sea, Okinawa, Hawaii. Two nucleotide substitutions found between sequenced coding regions S. anastomosans. Of 720...