A5100609678- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Bioinformatics and Genomic Networks
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Virology and Viral Diseases
- Plant Gene Expression Analysis
- Influenza Virus Research Studies
- Bacteriophages and microbial interactions
- Biopolymer Synthesis and Applications
- Postharvest Quality and Shelf Life Management
- Cancer Research and Treatments
- Nanoparticle-Based Drug Delivery
- Vector-Borne Animal Diseases
- Plant nutrient uptake and metabolism
- Animal Virus Infections Studies
- Bacterial Genetics and Biotechnology
- Plant biochemistry and biosynthesis
- Horticultural and Viticultural Research
- Viral Infections and Vectors
- S100 Proteins and Annexins
- Machine Learning in Bioinformatics
- Nematode management and characterization studies
Huazhong Agricultural University
2011-2021
Henan University
2021
Shanghai Jiao Tong University
2015-2018
Hubei Academy of Agricultural Sciences
2015
Animal Science Research Institute
2015
China Pharmaceutical University
2011
Symbiotic nitrogen fixation is an energy-expensive process, and the light available to plants has been proposed be a primary influencer. We demonstrate that light-induced soybean TGACG-motif binding factor 3/4 (GmSTF3/4) FLOWERING LOCUS T (GmFTs), which move from shoots roots, interdependently induce nodule organogenesis. Rhizobium-activated calcium- calmodulin-dependent protein kinase (CCaMK) phosphorylates GmSTF3, triggering GmSTF3–GmFT2a complex formation, directly activates expression of...
Citrus (Citrus spp.), a nonclimacteric fruit, is one of the most important fruit crops in global industry. However, biological behavior citrus ripening and postharvest senescence remains unclear. To better understand process we analyzed data sets from commercial microarrays, gas chromatography-mass spectrometry, liquid spectrometry validated physiological quality detection four main varieties genus Citrus. Network-based approaches mining modeling were used to investigate complex molecular...
Thermostable Newcastle disease virus (NDV) vaccines have been used widely to control for village poultry flocks, due their independence of cold chains delivery and storage. To explore the potential use thermostable NDV as a vaccine vector, an infectious clone avirulent strain TS09-C was developed using reverse genetics technology. The GFP gene, along with self-cleaving 2A gene foot-and-mouth ubiquitin monomer (2AUbi), were inserted immediately upstream NP (nucleocapsid protein), M (matrix...
The highly pathogenic avian influenza (HPAI) H5N1 virus is a virulent pathogen that causes respiratory diseases and death in humans other animal species worldwide. Because an enveloped virus, the entry, assembly, budding of particles are essential steps viral life cycle, relies on participation host cellular membrane proteins for all these steps. Thus, we took comparative proteomics approach by using 2-DE coupled with MALDI-TOF/TOF MS to profile involved infection at 6, 12, 24 h. Forty-two...
Sweet orange (Citrus sinensis) is one of the most important fruits world-wide. Because it a woody plant with long growth cycle, genetic studies sweet are lagging behind those other species.In this analysis, we employed ortholog identification and domain combination methods to predict protein-protein interaction (PPI) network for orange. The K-nearest neighbors (KNN) classification method was used verify filter network. final predicted PPI network, CitrusNet, contained 8,195 proteins 124,491...
As an industrial bacterium, Baccillus licheniformis DW2 produces bacitracin which is important antibiotic for many pathogenic microorganisms. Our previous study showed AbrB-knockout could significantly increase the production of bacitracin. Accordingly, it was meaningful to understand its genome features, expression differences between wild and (ΔAbrB) strains, regulation biosynthesis. Here, we sequenced, de novo assembled annotated genome, also sequenced transcriptomes in three growth...
Abstract In this study, we constructed a protein-protein interaction (PPI) network of B. licheniformis strain WX-02 with interolog method and domain-based method, which contained 15,864 edges 2,448 nodes. Although computationally predicted networks have relatively low coverage high false-positive rate, our prediction was confirmed from three perspectives: local structural features, functional similarities transcriptional correlations. Further analysis the COG heat map showed that protein...
The 2009 pandemic H1N1 influenza virus encodes an NS1 protein with 11 amino acids (aa) truncation at the C-terminus. C-terminal tail of constitutes a nucleolar localization signal (NoLS) and is binding domain cellular pre-mRNA processing protein, poly(A)-binding II (PABII). Here, our studies showed that C-terminal-truncated was inefficient blocking host gene expression, extension truncated to its full length increased inhibition expression. Mechanistically, this expression by full-length not...
The updated genome of Bacillus licheniformis WX‐02 comprises a circular chromosome 4 286 821 base‐pairs containing 4512 protein‐coding genes. We applied strand‐specific RNA‐sequencing to explore the transcriptome profiles B. under normal and high‐salt conditions (NaCl 6%). identified 2381 co‐expressed gene pairs constituting 871 operon structures. In addition, 1169 antisense transcripts 90 small RNAs were detected. Systematic comparison differentially expressed genes different revealed that...
Abstract We isolated Pseudomonas putida ( P. ) strain 1A00316 from Antarctica. This bacterium has a high efficiency against Meloidogyne incognita M. in vitro and under greenhouse conditions. The complete genome of was sequenced using PacBio single molecule real-time (SMRT) technology. A comparative genomic analysis 16 strains revealed that although belonged to , it phenotypically more similar nematicidal fluorescens strains. characterized the diversity specificity factors with genomics...
Carbamoyl-phosphate synthase plays a vital role in the carbon and nitrogen metabolism cycles. In Xanthomonas citri subsp. citri, carA carB encode small large subunits of carbamoyl-phosphate synthase, respectively. The deletion mutation coding regions revealed that did not affect any phenotypes, while played multiple roles pathogenicity. rendered loss pathogenicity host plants ability to induce hypersensitive reaction non-hosts. Quantitative reverse transcription-PCR assays indicated 11 hrp...
Xanthomonas citri subsp. (Xcc) is the major causal agent of citrus canker disease. The XAC1347 gene, which encodes a conserved membrane protein in Xcc, required for virulence during infection. However, molecular events mediated by remain unclear. In this study, we reported that gene positively regulated two component regulatory system ColRS and type III secretion function. A non-polar deletion mutant resulted Hrp minus phenotype plants reduced copper homeostasis. Real-time PCR experiments...
Background: Rapid accumulation of genomic and transcriptomic data initiates the development computational methods to identify regulation transcriptional factors (TF) genes. However, available display high false-positive rate unstable performance across different networks due their preferences for interactions with certain features. Model integration can reduce biases these improve specificity, especially pairwise whose correlations are very low. Objective: Different were compared in this...