A5065441101- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Plant responses to water stress
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Plant nutrient uptake and metabolism
- Microtubule and mitosis dynamics
- Postharvest Quality and Shelf Life Management
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Plant Surface Properties and Treatments
- Plant Parasitism and Resistance
- Mitochondrial Function and Pathology
- Plant biochemistry and biosynthesis
- Antioxidant Activity and Oxidative Stress
- Plant Pathogens and Fungal Diseases
- Machine Learning in Bioinformatics
- Circular RNAs in diseases
- Metabolomics and Mass Spectrometry Studies
Huazhong Agricultural University
2015-2025
First Affiliated Hospital of Xinxiang Medical University
2022-2025
Shanghai Zhangjiang Laboratory
2022-2025
South China Agricultural University
2020-2025
Henan Institute of Science and Technology
2023-2024
Wenzhou City People's Hospital
2024
Shandong University
2023
Nanjing Agricultural University
2021-2023
Second Hospital of Shandong University
2023
Weifang Maternity and Child Care Hospital
2020
Abstract Flowering plants have evolved numerous intraspecific and interspecific prezygotic reproductive barriers to prevent production of unfavourable offspring 1 . Within a species, self-incompatibility (SI) is widely utilized mechanism that rejects self-pollen 2,3 avoid inbreeding depression. Interspecific restrain breeding between species often follow the SI × self-compatible (SC) rule, is, pollen unilaterally incompatible (UI) on pistils but compatible (UC) SC 1,4–6 The molecular...
Summary Auxin is a major growth hormone in plants, and recent studies have elucidated many of the molecular mechanisms underlying its action, including transport, perception signal transduction. However, gaps remain our knowledge auxin biosynthetic control, partly due to complexity probable redundancy multiple pathways that involve YUCCA family flavin‐dependent mono‐oxygenases. This study reveals differential localization YUCCA4 alternative splice variants endoplasmic reticulum cytosol,...
Abstract The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both homologues yeast ARP2/3 activator, Pan1p. Here, we show that these also involved in actin cytoskeleton regulated autophagy. AtEH/Pan1 localise to plasma membrane autophagosomes. Upon induction autophagy, recruit TPC AP-2 subunits, clathrin, Increased expression boosts autophagosome formation, suggesting independent redundant pathways...
The endoplasmic reticulum (ER) is connected to the plasma membrane (PM) through plant-specific NETWORKED protein, NET3C, and phylogenetically conserved vesicle-associated protein-associated proteins (VAPs). Ten VAP homologues (VAP27-1 27-10) can be identified in Arabidopsis genome divided into three clades. Representative members from each clade were tagged with fluorescent protein expressed Nicotiana benthamiana. Proteins clades I III localized ER as well ER/PM contact sites (EPCSs),...
Primary plasmodesmata (PD) arise at cytokinesis when the new cell plate forms. During this process, fine strands of endoplasmic reticulum (ER) are laid down between enlarging Golgi-derived vesicles to form nascent PD, each pore containing a desmotubule, membranous rod derived from cortical ER. Little is known about forces that model ER during formation. Here, we show members reticulon (RTNLB) family ER-tubulating proteins in Arabidopsis (Arabidopsis thaliana) may play role formation...
Arabidopsis synaptotagmin 1 (SYT1) is localized on the endoplasmic reticulum-plasma membrane (ER-PM) contact sites in leaf and root cells. The ER-PM localization of SYT1 resembles that extended synaptotagmins (E-SYTs) animal In mammals, E-SYTs have been shown to regulate calcium signaling, lipid transfer, endocytosis. was reported be essential for maintaining cell integrity virus movement. This study provides detailed insight into subcellular VAP27-1, another ER-PM-tethering protein. VAP27-1...
Abstract Carotenoids play vital roles in the coloration of plant tissues and organs, particularly fruits; however, regulation carotenoid metabolism fruits during ripening is largely unknown. Here, we show that red light promotes fruit by inducing accelerated degreening accumulation kumquat fruits. Transcriptome profiling revealed a NAC (NAM/ATAF/CUC2) family transcription factor, FcrNAC22, specifically induced light-irradiated FcrNAC22 localizes to nucleus, its gene expression up-regulated...
Abstract ER-mitochondrial contact sites (EMCSs) are important for mitochondrial function. Here, we have identified a EMCS complex, comprising family of uncharacterised outer membrane proteins, TRB1, TRB2, and the ER protein, VAP27-1. In Arabidopsis, there three TraB isoforms trb1/trb2 double mutant exhibits abnormal morphology, strong starch accumulation, impaired energy metabolism, indicating that these proteins essential normal Moreover, TRB1 TRB2 also interact with ATG8 in order to...
The SCAR/WAVE complex is required for ARP2/3-mediated actin nucleation, and these complexes are highly conserved in plants animals [1Davidson A.J. Insall R.H. Actin-based motility: WAVE regulatory structure reopens old SCARs.Curr. Biol. 2011; 21: R66-R68Abstract Full Text PDF PubMed Scopus (19) Google Scholar, 2Deeks M.J. Hussey P.J. Arp2/3 SCAR: move to the fore.Nat. Rev. Mol. Cell 2005; 6: 954-964Crossref (58) Scholar]. Proteins from have been found be membrane associated [3Dyachok J. Shao...
Abstract Although abscisic acid (ABA) is a vital regulator of fruit ripening and several transcription factors have been reported to regulate ABA biosynthesis, reports the effect on citrus regulation its biosynthesis by multiple-transcription-factor complex are scarce. In present study, systematic metabolic, cytological, transcriptome analysis an ABA-deficient mutant (MT) Citrus reticulata cv. Suavissima confirmed positive process. The profiles indicated that CrNAC036 played important role...
Abstract Although multiple microscopic techniques have been applied to horticultural research, few studies of individual organelles in living fruit cells reported date. In this paper, we established an efficient system for the transient transformation citrus fruits using Agrobacterium -mediated method. Kumquat ( Fortunella crassifolia Swingle) was used; it exhibits higher efficiency than all that tested and a prolonged-expression window. Fruits were transformed with fluorescent reporters,...
Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles citrus, including long juvenility, polyembryony, and male/female sterility. The protoplast-based transient gene expression system is a powerful tool for functional characterization CRISPR/Cas9 genome editing higher plants, but it not citrus. In this study, polyethylene glycol (PEG)-mediated method was optimized citrus callus protoplast transfection, with an improved transfection efficiency of 68.4%....
Abstract Tomato (Solanum lycopersicum) fruit shape is related to microtubule organization and the activity of microtubule-associated proteins (MAPs). However, insights into mechanism formation from a cell biology perspective remain limited. Analysis tissue expression profiles different regulators revealed that functionally distinct classes MAPs, including members plant-specific MICROTUBULE-ASSOCIATED PROTEIN 70 (MAP70) IQ67 DOMAIN (IQD, also named SUN in tomato) families, are differentially...
Recent studies indicate that early postnatal period is a critical window for gut microbiota manipulation to optimise the immunity and body growth. This study investigated effects of maternal faecal orally administered neonatal piglets after birth on growth performance, selected microbial populations, intestinal permeability development mucosal immune system. In total, 12 litters crossbred newborn were in this study. Litter size was standardised 10 piglets. On day 1, each litter randomly...
A wide range of hemocyte types exist in insects but a full definition the different subclasses is not yet established. The current knowledge classification silkworm hemocytes mainly comes from morphology rather than specific markers, so our understanding detailed classification, lineage and functions very incomplete. Bombyx mori nucleopolyhedrovirus (BmNPV) representative member baculoviruses major pathogen that specifically infects silkworms ( ) causes serious losses sericulture industry....