A5083509921- Advances in Cucurbitaceae Research
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant Disease Management Techniques
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Nematode management and characterization studies
- Plant Molecular Biology Research
- Powdery Mildew Fungal Diseases
- Berry genetics and cultivation research
- Genomics and Phylogenetic Studies
- Cocoa and Sweet Potato Agronomy
- Plant Reproductive Biology
- Agronomic Practices and Intercropping Systems
- Insect-Plant Interactions and Control
- Plant Physiology and Cultivation Studies
- Potato Plant Research
- Seed Germination and Physiology
- Genetic diversity and population structure
- Nuts composition and effects
- Peanut Plant Research Studies
- Plant-Microbe Interactions and Immunity
U.S. Vegetable Laboratory
2016-2025
United States Department of Agriculture
2012-2025
Agricultural Research Service - Southeast Area
2025
Agricultural Research Service
2015-2024
Clemson University
2001-2017
Agricultural Research Organization
2006-2016
College of Charleston
2015
North Carolina State University
2001-2014
West Virginia State University
2006-2014
Universitat de Lleida
2014
Zhangjun Fei and colleagues report the draft genome of a Chinese elite watermelon inbred line 97103 resequencing 20 diverse accessions that represent three subspecies Citrullus lunatus. Comparative genome-wide analyses identify extent genetic diversity population structure germplasm. Watermelon, lanatus, is an important cucurbit crop grown throughout world. Here we high-quality sequence east Asia cultivar (2n = 2× 22) containing 23,440 predicted protein-coding genes. genomics analysis...
Fruit characteristics of sweet watermelon are largely the result human selection. Here we report an improved reference genome and whole-genome resequencing 414 accessions representing all extant species in Citrullus genus. Population genomic analyses reveal evolutionary history Citrullus, suggesting independent evolutions amarus lineage containing lanatus mucosospermus. Our findings indicate that different loci affecting fruit size have been under selection during speciation, domestication...
The Cucurbitaceae family (cucurbit) includes several economically important crops, such as melon, cucumber, watermelon, pumpkin, squash and gourds. During the past years, genomic genetic data have been rapidly accumulated for cucurbits. To store, mine, analyze, integrate disseminate these large-scale datasets to provide a central portal cucurbit research breeding community, we developed Cucurbit Genomics Database (CuGenDB; http://cucurbitgenomics.org) using Tripal toolkit. database currently...
Abstract The Cucurbitaceae (cucurbit) family consists of about 1,000 species in 95 genera, including many economically important and popular fruit vegetable crops. During the past several years, reference genomes have been generated for >20 cucurbit species, variome transcriptome profiling data rapidly accumulated cucurbits. To efficiently mine, analyze disseminate these large-scale datasets, we developed an updated version Cucurbit Genomics Database. database, CuGenDBv2...
Vegetable grafting began in the 1920s using resistant rootstock to control soilborne diseases. This process is now common Asia, parts of Europe, and Middle East. In Japan Korea, most cucurbits tomatoes ( Lycopersicon esculentum Mill.) grown are grafted. practice rare United States, there have been few experiments determine optimal production practices for different geographical climatic regions America. beginning change as a result phase out methyl bromide. The U.S. cucurbit tomato...
The primary purpose of grafting vegetables worldwide has been to provide resistance soilborne diseases. potential loss methyl bromide as a soil fumigant combined with pathogen commonly used pesticides will make pathogens even more important in the future. major disease problems addressed by include fusarium wilt, bacterial verticillium monosporascus root rot, and nematodes. Grafting also shown some instances increase tolerance foliar fungal diseases, viruses, insects. If area devoted...
As part of our ongoing efforts to sequence and map the watermelon (Citrullus spp.) genome, we have constructed a high density genetic linkage map. The positioned 234 genome scaffolds (an average size 1.41 Mb) that cover about 330 Mb account for 93.5% 353 assembled genomic sequences elite Chinese line 97103 lanatus var. lanatus). was using an F8 population 103 recombinant inbred lines (RILs). RILs are derived from cross between United States Plant Introduction (PI) 296341-FR (C. citroides)...
How sugar transporters regulate accumulation in fruits is poorly understood and particularly so for species storing high-concentration Suc. Accumulation of soluble sugars watermelon (Citrullus lanatus) fruit, a major quality trait, had been selected during domestication. Still, the molecular mechanisms controlling this quantitative trait are unknown. We resequenced 96 recombinant inbred lines, derived from crossing sweet unsweet accessions, to narrow down size previously described content...
Years of selection for desirable fruit quality traits in dessert watermelon (Citrullus lanatus) has resulted a narrow genetic base modern cultivars. Development novel genomic and resources offers great potential to expand diversity improve important watermelon. Here, we report high-quality genome sequence cultivar 'Charleston Gray', principal American watermelon, complement the existing reference from '97103', an East Asian cultivar. Comparative analyses between genomes Gray' '97103'...
Bottle gourd (Lagenaria siceraria) is an important vegetable crop as well a rootstock for other cucurbit crops. In this study, we report high-quality 313.4-Mb genome sequence of bottle inbred line, USVL1VR-Ls, with scaffold N50 8.7 Mb and the longest 19.0 Mb. About 98.3% assembled scaffolds are anchored to 11 pseudomolecules. Our comparative genomic analysis identifies chromosome-level syntenic relationships between cucurbits, lineage-specific gene family expansions in gourd. We...
Color and pigment contents are important aspects of fruit quality consumer acceptance cucurbit crops. Here, we describe the independent mapping cloning a common causative APRR2 gene regulating accumulation in melon watermelon. We initially show that transcription factor is for qualitative difference between dark light green rind both Further analyses establish link sequence or expression level variations CmAPRR2 content flesh mature fruits. A genome-wide association study (GWAS) young color...
Genetic diversity and relatedness were assessed among 46 American cultivars of watermelon ( Citrullus lanatus var. ), 12 U.S. Plant Introduction accessions (PIs) sp. using 25 randomly amplified polymorphic DNA (RAPD) primers. These primers produced 288 distinct reproducible bands that could be scored with high confidence PIs. Based on the RAPD data, genetic similarity coefficients calculated a dendrogram was constructed unweighted pair-group method arithmetic average (UPGMA). The C. PIs...
Four bottle gourd (Lagenaria siceraria) cultivars, one squash (Cucurbita moschata × C. maxima) hybrid, five wild watermelon (Citrullus lanatus var. citroides) germplasm lines, and commercial (C. cultivar were evaluated as rootstocks for cultivated lanatus) in fields infested with the southern root-knot nematode (Meloidogyne incognita) Charleston, SC 2007 2008, Quincy, FL 2008. Commercial 'Fiesta' (diploid seeded) 'Tri-X 313' (triploid seedless) scions grafted onto respectively. In 2007,...
A large single nucleotide polymorphism (SNP) dataset was used to analyze genome-wide diversity in a diverse collection of watermelon cultivars representing globally cultivated, genetic diversity. The marker density required for conducting successful association mapping depends on the extent linkage disequilibrium (LD) within population. Use genotyping by sequencing reveals numbers SNPs that turn generate opportunities and marker-assisted selection, even crops such as which few genomic...
Melon (Cucumis melo L.) is a phenotypically diverse eudicot diploid (2n = 2x 24) has climacteric and non-climacteric morphotypes show wide variation for fruit firmness, an important trait transportation shelf life. We generated 13,789 SNP markers using genotyping-by-sequencing (GBS) anchored them to chromosomes understand genome-wide fixation indices (Fst) between various melon genomewide linkage disequilibrium (LD) decay. The FST accessions of cantalupensis inodorus was 0.23. agrestis in...
Abstract Background Cultivated watermelon form large fruits that are highly variable in size, shape, color, and content, yet have extremely narrow genetic diversity. Whereas a plethora of genes involved cell wall metabolism, ethylene biosynthesis, fruit softening, secondary metabolism during development ripening been identified other plant species, little is known the these processes watermelon. A microarray quantitative Real-Time PCR-based study was conducted [ Citrullus lanatus (Thunb.)...
Fusarium wilt (FW) is a major disease of watermelon in North America and around the world. Control this difficult because soilborne causal agent oxysporum f. sp. niveum ( Fon ) produces chlamydospores that remain infectious soil for many years. Although various levels resistance to Races 0 1 exist cultivars, no Race 2 or 3 has been reported. In study, we used seed seedling inoculation procedures screen 110 U.S. PIs wild Citrullus lanatus var. citroides FW. Of these accessions, 15 showed...
The plant microbiome is a key determinant of health and productivity, changes in the can alter tolerance to biotic abiotic stresses quality end produce. Little known about microbial diversity its effect on carbohydrate metabolism ripe fruits. In this study, we aimed understand function microorganisms relation watermelon We used 16S metagenomics RNAseq metatranscriptomics for analysis red (PI459074, Congo, SDRose) yellow fruit-flesh cultivars (PI227202, PI435990, JBush) geographically...
The exploitation of synthetic polyploids for producing seedless fruits is well known in watermelon. Tetraploid progenitors triploid watermelon plants, compared with their diploid counterparts, exhibit wide phenotypic differences. Although many factors modulate alternative splicing (AS) the effects autopolyploidization on AS are still unknown. In this study, we used tissues leaf, stem, and fruit tetraploid sweet to understand changes gene expression occurrence AS. RNA-sequencing analysis was...
When infecting a host plant, the fungus Fusarium oxysporum secretes several effector proteins into xylem tissue to promote virulence. However, in plant with an innate immune system involving analogous resistance proteins, may trigger resistance, rather than promoting Identity of genes f. sp. niveum (Fon) races that affect watermelon (Citrullus lanatus) are currently unknown. In this study, SIX6 (secreted protein 6) gene was identified Fon 0 and 1 but not more virulent race 2. Disrupting...