A5060341139- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Advances in Cucurbitaceae Research
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- CRISPR and Genetic Engineering
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Microbial Metabolites in Food Biotechnology
- Cocoa and Sweet Potato Agronomy
- Islanding Detection in Power Systems
- Banana Cultivation and Research
- Insect Resistance and Genetics
- Powdery Mildew Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Protein Tyrosine Phosphatases
- Potato Plant Research
- Horticultural and Viticultural Research
- Sugarcane Cultivation and Processing
Beijing Academy of Agricultural and Forestry Sciences
2016-2024
China Agricultural University
2012
The phytohormone ethylene regulates multiple aspects of plant growth and development responses to environmental stress. However, the exact role in freezing stress remains unclear. Here, we report that negatively Arabidopsis thaliana. Freezing tolerance was decreased overproducer1 by application precursor 1-aminocyclopropane-1-carboxylic acid but increased addition biosynthesis inhibitor aminoethoxyvinyl glycine or perception antagonist Ag+. Furthermore, ethylene-insensitive mutants,...
Fruit characteristics of sweet watermelon are largely the result human selection. Here we report an improved reference genome and whole-genome resequencing 414 accessions representing all extant species in Citrullus genus. Population genomic analyses reveal evolutionary history Citrullus, suggesting independent evolutions amarus lineage containing lanatus mucosospermus. Our findings indicate that different loci affecting fruit size have been under selection during speciation, domestication...
How sugar transporters regulate accumulation in fruits is poorly understood and particularly so for species storing high-concentration Suc. Accumulation of soluble sugars watermelon (Citrullus lanatus) fruit, a major quality trait, had been selected during domestication. Still, the molecular mechanisms controlling this quantitative trait are unknown. We resequenced 96 recombinant inbred lines, derived from crossing sweet unsweet accessions, to narrow down size previously described content...
How raffinose (Raf) family oligosaccharides, the major translocated sugars in vascular bundle cucurbits, are hydrolyzed and subsequently partitioned has not been fully elucidated. By performing reciprocal grafting of watermelon (Citrullus lanatus) fruits to branch stems, we observed that Raf was fruit cultivar watermelons but backlogged wild ancestor species. Through a genome-wide association study, alkaline alpha-galactosidase ClAGA2 identified as key factor controlling stachyose...
Watermelon (Citrullus lanatus, 2n = 2x 22) is an economically important Cucurbitaceae species that widely cultivated worldwide. Commercial watermelon cultivars are usually F1 hybrids due to harness heterosis, which used pure lines as parents. However, obtaining inbred requires at least eight generations of self-pollinating, time-consuming, resource-intensive and cost-prohibitive. By contrast, doubled haploid (DH) technology provides homozygous within two generations. Parthenogenesis the...
Abstract Abscisic acid (ABA) is a critical regulator of seed development and germination. β-glucosidases (BGs) have been suggested to be contributors increased ABA content because they catalyze the hydrolysis ABA-glucose ester release free ABA. However, whether BGs are involved in unclear. In this study, candidate gene, ClBG1 , watermelon was selected for targeted mutagenesis via CRISPR/Cas9 system. Seed size weight were significantly reduced Clbg1 -mutant lines, which mainly attributed...
Summary To understand sex determination in watermelon ( Citrullus lanatus ), a spontaneous gynoecious mutant, XHBGM, was selected from the monoecious wild type XHB. Using map‐based cloning, resequencing and fluorescence situ hybridization analysis, unique chromosome translocation between 2 3 found XHBGM. Based on breakpoint location 2, putative C2H2 zinc finger transcription factor gene, ClWIP1 (gene ID Cla008537 an orthologue of melon gynoecy gene CmWIP1 , disrupted. clustered regularly...
Unloading sugar from sink phloem by transporters is complex and much remains to be understood about this phenomenon in the watermelon fruit. Here, we report a novel vacuolar transporter (ClVST1) identified through map-based cloning association study, whose expression fruit associated with accumulation of sucrose (Suc) ClVST197 knockout lines show decreased content total biomass, whereas overexpression increases Suc content. Population genomic subcellular localization analyses strongly...
The NAC transcription factor NONRIPENING (NOR) is a master regulator of climacteric fruit ripening. Melon (Cucumis melo L.) has and non-climacteric ripening varieties an ideal model to study Two natural CmNAC-NOR variants, the haplotype CmNAC-NORS,N CmNAC-NORA,S , have effects on ripening; however, their regulatory mechanisms not been elucidated. Here, we report that mutation in transcriptional activation domain contributes melon knockout climacteric-type cultivar "BYJH" completely inhibited...
Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is a non-climacteric fruit. The modern sweet-dessert watermelon the result of years cultivation and selection for fruits with desirable qualities. To date, mechanisms fruit ripening, role abscisic acid (ABA) in this process, has not been well understood. We quantified levels free conjugated ABA contents cultivated (97103; C. subsp. vulgaris), semi-wild germplasm (PI179878; mucosospermus), wild (PI296341-FR; lanatus). Results showed that...
There has been no systematic research on the effect of plant growth regulators and silver nitrate treatments control sex expression in watermelon. In this study, we tested responses four watermelon forms (monoecism, gynoecism, andromonoecism, hermaphrodite) to gibberellin, ethephon treatments. Results have shown that, monoecious plants, gibberellins (GA3) reduced percentage female flowers delayed occurrence first flower, while induced formation bisexual flowers. gynoecious both transformed...
Although it has been reported previously that ethylene plays a critical role in sex determination cucurbit species, how the andromonoecy carries both male and hermaphroditic flowers is determined watermelon still unknown. Here we showed gene 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4), expressed specifically carpel primordia, determines watermelon. Among four single nucleotide polymorphism (SNPs) one InDel identified coding region of CitACS4, C364W mutation located conserved box 6...
In this study, we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower. Acetolactate synthase (ALS) and Centromere-specific histone H3 variant (CENH3) genes were selected as base-editing targets hypocotyls of cauliflower explants. For ALS gene, a C-to-T conversion Pro182 codon (CCT) can alter encoded amino acid, likely resulting herbicide resistance, mutation Leu133 (CTT) CENH3 gene may haploid inducer. Results indicated that transformation efficiency was...
Watermelon (Citrullus lanatus) as non-climacteric fruit is domesticated from the ancestors with inedible fruits. We previously revealed that abscisic acid (ABA) signaling pathway gene ClSnRK2.3 might influence watermelon ripening. However, molecular mechanisms are unclear. Here, we found selective variation of resulted in lower promoter activity and expression level cultivated watermelons than ancestors, which indicated be a negative regulator Overexpression (OE) significantly delayed...
Pericarp color is a crucial commercial trait influencing consumer preferences for bitter gourds. However, until now, the gene responsible this has remained unidentified. In study, we identified (McAPRR2) controlling pericarp via genome-wide association study (GWAS) utilizing resequencing data of 106 gourd accessions. McAPRR2 exhibits three primary haplotypes: Hap1 wild type with green pericarp, Hap2 SA (South Asian) and SEA (Southeast Asia) Hap3 primarily light pericarp. The haplotype...
Watermelon fruit undergoes distinct development stages with dramatic changes during ripening. To date, the molecular mechanics of watermelon ripening remain unclear. Genetic and transcriptome evidences suggested that ethylene response factor (ERF) gene ClERF069 may be an important candidate affecting dissect roles in ripening, structure phylogenetic analysis were performed using amplified full-length sequence. Normal-ripening 97103, non-ripening PI296341-FR RIL population used to analyze...
Fruit ripening is a highly complicated process, which modulated by phytohormones, signal regulators and environmental factors playing in an intricate network that regulates ripening-related genes expression. Although transcriptomics effective tool to predict protein levels, abundances are also extensively affected post-transcriptional post-translational regulations. Here, we used RNA sequencing (RNA-seq) tandem mass tag (TMT)-based quantitative proteomics study the comprehensive mRNA...
The regulation of non-climacteric fruit ripening by the transcription factor NON-RIPENING (NOR) is poorly understood. Here, we identified that NOR homolog in watermelon (Citrullus lanatus) was located within selective sweep and sweetness quantitative trait locus selected during domestication from landraces to cultivars. ClNOR knockout substantially delayed ripening, fruits plants had lower abscisic acid (ABA) levels, lighter colored flesh, were less sweet compared wild type. Transcriptome...
Fruit ripening is a highly complicated process that accompanied by the formation of fruit quality. In recent years, series studies have demonstrated post-transcriptional control play important roles in and quality formation. Till now, mechanisms for watermelon not been comprehensively studied. this study, we conducted PacBio single-molecule long-read sequencing to identify genome-wide alternative splicing (AS), polyadenylation (APA) long non-coding RNAs (lncRNAs) fruit. total, 6,921,295...