A5100764105- Advances in Cucurbitaceae Research
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Postharvest Quality and Shelf Life Management
- Plant Physiology and Cultivation Studies
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
- Genetic Mapping and Diversity in Plants and Animals
- Plant-Microbe Interactions and Immunity
- Powdery Mildew Fungal Diseases
- Cocoa and Sweet Potato Agronomy
- Plant Pathogenic Bacteria Studies
- Phytochemistry and Biological Activities
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Fungal Biology and Applications
- Food Quality and Safety Studies
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Plant Reproductive Biology
Ganzhou People's Hospital
2025
Shanghai Tenth People's Hospital
2025
Shandong University of Technology
2025
Beijing Academy of Agricultural and Forestry Sciences
2015-2024
Gansu Academy of Agricultural Sciences
2024
Institute of Plant Protection
2024
Sichuan Agricultural University
2020-2024
Dezhou University
2013-2024
Nanjing Brain Hospital
2024
Nanjing Medical University
2024
Zhangjun Fei and colleagues report the draft genome of a Chinese elite watermelon inbred line 97103 resequencing 20 diverse accessions that represent three subspecies Citrullus lunatus. Comparative genome-wide analyses identify extent genetic diversity population structure germplasm. Watermelon, lanatus, is an important cucurbit crop grown throughout world. Here we high-quality sequence east Asia cultivar (2n = 2× 22) containing 23,440 predicted protein-coding genes. genomics analysis...
The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and moschata provide evidence supporting an allotetraploidization event Cucurbita. We are able to partition into two homoeologous subgenomes based on different genetic distances melon, cucumber, watermelon Benincaseae tribe. estimate that diploid progenitors successively diverged from around 31 26 million years ago (Mya), respectively,...
Fruit characteristics of sweet watermelon are largely the result human selection. Here we report an improved reference genome and whole-genome resequencing 414 accessions representing all extant species in Citrullus genus. Population genomic analyses reveal evolutionary history Citrullus, suggesting independent evolutions amarus lineage containing lanatus mucosospermus. Our findings indicate that different loci affecting fruit size have been under selection during speciation, domestication...
Abstract Background A number of molecular marker linkage maps have been developed for melon ( Cucumis melo L .) over the last two decades. However, these were constructed using different sets, thus, making comparative analysis among difficult. In order to solve this problem, a consensus genetic map in was primarily highly transferable anchor markers that broad potential use mapping, synteny, and quantitative trait loci (QTL) analysis, increasing breeding effectiveness efficiency via...
As part of our ongoing efforts to sequence and map the watermelon (Citrullus spp.) genome, we have constructed a high density genetic linkage map. The positioned 234 genome scaffolds (an average size 1.41 Mb) that cover about 330 Mb account for 93.5% 353 assembled genomic sequences elite Chinese line 97103 lanatus var. lanatus). was using an F8 population 103 recombinant inbred lines (RILs). RILs are derived from cross between United States Plant Introduction (PI) 296341-FR (C. citroides)...
How sugar transporters regulate accumulation in fruits is poorly understood and particularly so for species storing high-concentration Suc. Accumulation of soluble sugars watermelon (Citrullus lanatus) fruit, a major quality trait, had been selected during domestication. Still, the molecular mechanisms controlling this quantitative trait are unknown. We resequenced 96 recombinant inbred lines, derived from crossing sweet unsweet accessions, to narrow down size previously described content...
Abstract Background Cultivated watermelon [ Citrullus lanatus (Thunb.) Matsum. & Nakai var. ] is an important agriculture crop world-wide. The fruit of undergoes distinct stages development with dramatic changes in its size, color, sweetness, texture and aroma. In order to better understand the genetic molecular basis these significantly expand transcript catalog, we have selected four critical used Roche/454 next-generation sequencing technology generate a large expressed sequence tag...
Abstract Background Modern watermelon ( Citrullus lanatus L. ) cultivars share a narrow genetic base due to many years of selection for desirable horticultural qualities. Wild subspecies within C. are important potential sources novel alleles breeding, but successful trait introgression into elite has had limited success. The application marker assisted (MAS) in is yet be realized, mainly the past lack high quality maps. Recently, number useful maps have become available, however these few...
Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an important vegetable crop world-wide. fruit quality a complex trait determined by various factors such as sugar content, flesh color and texture. Fruit developmental process of cultivated wild watermelon are highly different. To systematically understand the molecular basis these differences, we compared transcriptome profiles tissues 97103 PI296341-FR. We identified 2,452, 826 322 differentially expressed genes in flesh, mesocarp...
How raffinose (Raf) family oligosaccharides, the major translocated sugars in vascular bundle cucurbits, are hydrolyzed and subsequently partitioned has not been fully elucidated. By performing reciprocal grafting of watermelon (Citrullus lanatus) fruits to branch stems, we observed that Raf was fruit cultivar watermelons but backlogged wild ancestor species. Through a genome-wide association study, alkaline alpha-galactosidase ClAGA2 identified as key factor controlling stachyose...
Abstract NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in fruit ripening quality. The watermelon genome encodes 80 genes, 21 of these genes are highly expressed both the flesh vascular tissues. Among ClNAC68 expression was significantly higher than rind. However, intrinsic regulatory mechanism quality is still unknown. In this study, we found that a transcriptional repressor repression domain located C-terminus. Knockout by CRISPR-Cas9 system decreased soluble solid...
Watermelon (Citrullus lanatus, 2n = 2x 22) is an economically important Cucurbitaceae species that widely cultivated worldwide. Commercial watermelon cultivars are usually F1 hybrids due to harness heterosis, which used pure lines as parents. However, obtaining inbred requires at least eight generations of self-pollinating, time-consuming, resource-intensive and cost-prohibitive. By contrast, doubled haploid (DH) technology provides homozygous within two generations. Parthenogenesis the...
Pumpkin (Cucurbita maxima Duch.) is an economically important crop belonging to the Cucurbitaceae family. However, very few genomic and genetic resources are available for this species. As part of our ongoing efforts sequence pumpkin genome, high-density map essential anchoring orienting assembled scaffolds. In addition, a saturated can facilitate quantitative trait locus (QTL) mapping. A set 186 F2 plants derived from cross inbred lines Rimu SQ026 were genotyped using...
Red-fleshed watermelons (Citrullus lanatus) that accumulate lycopene in their flesh cells have been selected and domesticated from pale-fleshed ancestors. However, the molecular basis of this trait remains poorly understood. Using map-based cloning transgenic analysis, we identified a β-cyclase (ClLCYB) gene controls color watermelon. Down-regulation ClLCYB caused to change pale yellow red, overexpression red-fleshed line orange. Analysis single-nucleotide polymorphisms using 211 watermelon...
Abstract Abscisic acid (ABA) is a critical regulator of seed development and germination. β-glucosidases (BGs) have been suggested to be contributors increased ABA content because they catalyze the hydrolysis ABA-glucose ester release free ABA. However, whether BGs are involved in unclear. In this study, candidate gene, ClBG1 , watermelon was selected for targeted mutagenesis via CRISPR/Cas9 system. Seed size weight were significantly reduced Clbg1 -mutant lines, which mainly attributed...
Chromoplast development plays a crucial role in controlling carotenoid content watermelon flesh. Modern cultivated watermelons with colorful flesh are believed to originate from pale-colored and no-sweet progenitors. But the molecular basis of color formation regulation is poorly understood. More chromoplasts released globules were observed red-fleshed fruit 97103 cultivar than fruits PI296341-FR line. Transcriptome profiles these two materials identified Cla017962, predicted as ClPHT4;2,...
Summary To understand sex determination in watermelon ( Citrullus lanatus ), a spontaneous gynoecious mutant, XHBGM, was selected from the monoecious wild type XHB. Using map‐based cloning, resequencing and fluorescence situ hybridization analysis, unique chromosome translocation between 2 3 found XHBGM. Based on breakpoint location 2, putative C2H2 zinc finger transcription factor gene, ClWIP1 (gene ID Cla008537 an orthologue of melon gynoecy gene CmWIP1 , disrupted. clustered regularly...
Unloading sugar from sink phloem by transporters is complex and much remains to be understood about this phenomenon in the watermelon fruit. Here, we report a novel vacuolar transporter (ClVST1) identified through map-based cloning association study, whose expression fruit associated with accumulation of sucrose (Suc) ClVST197 knockout lines show decreased content total biomass, whereas overexpression increases Suc content. Population genomic subcellular localization analyses strongly...
The study of yield traits can reveal the genetic architecture grain for improving maize production. In this study, an association panel comprising 362 inbred lines and a recombinant line population derived from X178 × 9782 were used to identify candidate genes nine traits. High-priority overlap (HPO) genes, which are prioritized in genome-wide (GWAS), investigated using coexpression networks. GWAS identified 51 environmentally stable SNPs two environments 36 pleiotropic SNPs, including three...
The NAC transcription factor NONRIPENING (NOR) is a master regulator of climacteric fruit ripening. Melon (Cucumis melo L.) has and non-climacteric ripening varieties an ideal model to study Two natural CmNAC-NOR variants, the haplotype CmNAC-NORS,N CmNAC-NORA,S , have effects on ripening; however, their regulatory mechanisms not been elucidated. Here, we report that mutation in transcriptional activation domain contributes melon knockout climacteric-type cultivar "BYJH" completely inhibited...
Arbuscular mycorrhizal fungi (AMF) perform a vital role in terrestrial ecosystems.To investigate the diversity of AMF communities on western slope Helan Mountain at different altitudes and their influence factors, high-throughput sequencing was used to study structure soil under environments interrelationships between environmental factors.The results revealed that there were significant differences (p < 0.05) physical chemical properties along altitudes. A total 1,145 OTUs obtained by...