A5101723472- Photosynthetic Processes and Mechanisms
- Food composition and properties
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Biofuel production and bioconversion
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- DNA Repair Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Plant and animal studies
- Molecular Biology Techniques and Applications
- Phytochemicals and Antioxidant Activities
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Insect Resistance and Genetics
- Cellular transport and secretion
- Carcinogens and Genotoxicity Assessment
- Endoplasmic Reticulum Stress and Disease
- Biochemical Analysis and Sensing Techniques
- RNA Interference and Gene Delivery
Chinese Academy of Agricultural Sciences
2022-2025
Ministry of Agriculture and Rural Affairs
2022-2025
Wuhan Botanical Garden
2025
Oil Crops Research Institute
2025
Shanxi University
2015-2024
Institute of Crop Sciences
2023-2024
Sweet Potato Research Institute
2013-2023
Beijing Forestry University
2014-2023
Jiangnan University
2023
State Key Laboratory of Food Science and Technology
2023
A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion human genome was generated by whole-genome shotgun sequencing method. The 14.8-billion bp DNA over 9 months from 27,271,853 high-quality reads (5.11-fold coverage genome) both ends plasmid clones made five individuals. Two assembly strategies—a and a regional chromosome assembly—were used, each combining data Celera publicly funded effort. public were shredded into 550-bp segments to create 2.9-fold those regions...
Wheat (Triticum aestivum L.) lines T1, T4, and T6 were genetically modified to increase glycine betaine (GB) synthesis by introduction of the BADH (betaine aldehyde dehydrogenase, BADH) gene from mountain spinach (Atriplex hortensis L.). These transgenic WT wheat (T. used study effect increased GB on tolerance salt stress. Salt stress due 200 mmol L− 1 NaCl impaired photosynthesis four lines, as indicated declines in net photosynthetic rate (Pn), stomatal conductance (Gs), maximum...
Brassica oleracea includes several morphologically diverse, economically important vegetable crops, such as the cauliflower and cabbage. However, genetic variants, especially large structural variants (SVs), that underlie extreme morphological diversity of B. remain largely unexplored.Here we present high-quality chromosome-scale genome assemblies for two morphotypes, Direct comparison these identifies ~ 120 K high-confidence SVs. Population analysis 271 accessions using SVs clearly...
Abstract In cereal grains, starch is synthesized by the concerted actions of multiple enzymes on surface granules within amyloplast. However, little known about how starch-synthesizing access granules, especially for amylopectin biosynthesis. Here, we show that rice (Oryza sativa) floury endosperm9 (flo9) mutant defective in biosynthesis, leading to grains exhibiting a endosperm with hollow core. Molecular cloning revealed FLO9 encodes plant-specific protein homologous Arabidopsis...
Summary Endosperm is the main storage organ in cereal grain and determines yield quality. The molecular mechanisms of heat shock proteins regulating starch biosynthesis endosperm development remain obscure. Here, we report a rice floury mutant flo24 that develops abnormal grains central starchy cells. Map‐based cloning complementation test showed FLO24 encodes protein HSP101, which localized plastids. mutated T296I dramatically lost its ability to hydrolyze ATP rescue thermotolerance defects...
DNA interstrand cross-links (ICLs) block the strand separation necessary for essential functions such as transcription and replication and, hence, represent an important class of lesion. Since both strands double helix are affected in cross-linked DNA, it is likely that conservative recombination using undamaged homologous regions a donor may be required to repair ICLs error-free manner. However, Escherichia coli yeast, recombination-independent mechanisms ICL have been identified addition...
The starch properties of the storage root (SR) affect quality sweet potato (Ipomoea batatas (L.) Lam.). Although numerous studies have analyzed accumulation and in SRs, transcriptomic variation associated with SR has not been quantified. In this study, we measured sugar contents transcriptome profiles SRs harvested from potatoes high, medium, extremely low contents, at five developmental stages (65, 80, 95, 110, 125 days after transplanting (DAP)). We found that differences both water...
Abstract Crape myrtle (Lagerstroemia indica) is a globally used ornamental woody plant and the representative species of Lagerstroemia. However, studies on evolution genomic breeding L. indica have been hindered by lack reference genome. Here we assembled first high-quality genome using PacBio combined with Hi-C scaffolding to anchor 329.14-Mb assembly into 24 pseudochromosomes. We detected previously undescribed independent whole-genome triplication event occurring 35.5 million years ago in...
CYP78A, a cytochrome P450 subfamily that includes rice (Oryza sativa L.) BIG GRAIN2 (BG2, CYP78A13) and Arabidopsis thaliana KLUH (KLU, CYP78A5), generate an unknown mobile growth signal (referred to as CYP78A-derived signal) increases grain (seed) size. However, the mechanism by which CYP78A pathway size remains elusive. Here, we characterized small mutant, grain4 (smg4), with smaller grains than its wild type due restricted cell expansion proliferation in spikelet hulls. SMG4 encodes...
Flavonol synthase (FLS) is a key enzyme of the flavonoid biosynthetic pathway, which catalyzes conversion dihydroflavonols into flavonols. In this study, FLS gene IbFLS1 was cloned and characterized from sweet potato. The resulting protein showed high similarity with other plant FLSs. conserved amino acids (HxDxnH motifs) binding ferrous iron residues (RxS 2-oxoglutarate were found in at positions, as FLSs, suggesting that belongs to 2-oxoglutarate-dependent dioxygenases (2-ODD) superfamily....
It is known that red light irradiation enhances the biosynthesis of (E)-β-caryophyllene in plants. However, underlying mechanism connecting to remains elusive. This study reveals a molecular cascade involving phyB-PIF4-MYC2 module, which regulates response signal Arabidopsis thaliana. In this phyB positively under light, whereas PIF4 negatively it; both regulations require involvement MYC2, transcription factor can bind directly promoter TPS21 gene encodes synthase. Importantly,...
In the past five years, number of articles related to ultrasonic mineral flotation has increased by about 50 per year, and overall trend is on rise. The most recent developments in ultrasonics for process intensification are reviewed herein, including effects ultrasound treatment an aqueous slurry, improvement methods technological processes, device development tracking, application engineering. At this point time, there pilot-scale tests evaluate feasibility pretreatment technology...
The phytohormone cytokinin (CK) positively regulates the activity of inflorescence meristem (IM). Cytokinin oxidase 2/Grain number 1a (OsCKX2/Gn1a)-mediated degradation CK in rice (Oryza sativa L.) negatively panicle grain number, whereas DENSE AND ERECT PANICLE 1 (DEP1) per (GNP). However, detailed regulatory mechanism between DEP1 and OsCKX2 remains elusive. Here, we report GRAS (GIBBERELLIN ACID INSENSITIVE, REPRESSOR OF GA1, SCARECROW) transcription factor GRAIN NUMBER ASSOCIATED (GNA),...
Abstract Ion mobility spectrometer (IMS) has been widely deployed for on-site detection of explosives. The common nitro-based explosives are usually detected by negative IMS while the emerging peroxide-based better positive IMS. In this study, a fast polarity-switchable was constructed to detect these two explosive species in single measurement. As large traditional Faraday detector would cause trailing reactant ion peak (RIP), with focusing vicinity developed reducing radius 3.3 mm and...
ABSTRACT Autophagy is a highly conserved cellular program in eukaryotic cells which mediates the degradation of cytoplasmic components through lysosome, also named vacuole plants. However, molecular mechanisms underlying fusion autophagosomes with remain unclear. Here, we report functional characterization rice ( Oryza sativa ) mutant defects storage protein transport endosperm and accumulation numerous root cells. Cytological immunocytochemical experiments showed that this exhibits defect...
This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery placenta mesenchymal stem cells (PMSCs). By using disulfide exchange between SPDP-activated PEI-β-CyD and peptide, TAT-PEI-β-CyD polymer was fabricated success of this confirmed by presence characteristic peaks PEI (at δ 2.8–3.2 ppm), CyD 5.2, 3.8–4.0 3.4–3.6 ppm) 1.6–1.9 6.8–7.2 in 1H NMR spectrum TAT-PEI-β-CyD. The polymer–plasmid–DNA polyplex could condense at an N/P ratio...
Grain physicochemical properties determine the table quality of fresh waxy maize. Two maize varieties, Suyunuo 5 (shading tolerant) and FHN003 sensitive), were used to estimate effect shading (plants received 30% less radiation than control) during grain filling (from 0 d 23 after pollination) on grain. Shading decreased weight by 8.4 19.1%, respectively. increased water content FHN003, whereas that was not affected. In both varieties for treatment, soluble sugar, starch protein contents...