A5061054593- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neurobiology and Insect Physiology Research
- DNA Repair Mechanisms
- Prostate Cancer Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Prostate Cancer Treatment and Research
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Proteomics Techniques and Applications
- Animal Genetics and Reproduction
- Plant Genetic and Mutation Studies
- Plant and animal studies
- Cancer Genomics and Diagnostics
- Evolution and Genetic Dynamics
- Genetic Mapping and Diversity in Plants and Animals
- Gene expression and cancer classification
- Signaling Pathways in Disease
- Genetics, Bioinformatics, and Biomedical Research
- BRCA gene mutations in cancer
- Single-cell and spatial transcriptomics
Cabrini Hospital
2013-2019
Monash University
2013-2019
Australian National University
1988-1998
Neurosciences Institute
1996-1998
University of Hawaii System
1995-1998
University of Hawaiʻi at Mānoa
1998
Kyoto Institute of Technology
1997
John Jay College of Criminal Justice
1997
Baylor College of Medicine
1995
Université Libre de Bruxelles
1993
A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion human genome was generated by whole-genome shotgun sequencing method. The 14.8-billion bp DNA over 9 months from 27,271,853 high-quality reads (5.11-fold coverage genome) both ends plasmid clones made five individuals. Two assembly strategies—a and a regional chromosome assembly—were used, each combining data Celera publicly funded effort. public were shredded into 550-bp segments to create 2.9-fold those regions...
ABSTRACT By combining elements of two Y-autosome translocations with displaced autosomal breakpoints, it is possible to produce zygotes heterozygous for a deficiency the region between and also, as complementary product, carrying duplication precisely same region. A set appropriately positioned therefore, can in principle be used generate non-overlapping deficiencies duplications entire complement.—Using this method, we have succeeded examining segmental aneuploids 85% chromosomes 2 3 order...
The high degree of similarity between the mouse and human genomes is demonstrated through analysis sequence chromosome 16 (Mmu 16), which was obtained as part a whole-genome shotgun assembly genome. genome about 10% smaller than genome, owing to lower repetitive DNA content. Comparison structure protein-coding potential Mmu with that homologous segments identifies regions conserved synteny chromosomes (Hsa) 3, 8, 12, 16, 21, 22. Gene content order are highly syntenic blocks Of 731 predicted...
Saccharomyces cerevisiae contains 62 molecules per cell of a class covalently closed circular DNA termed omicron ( o DNA) buoyant density 1.701 g/cm 3 with major size 1.9 μm. The localization has been investigated in respiratory deficient mutant 2eρ −1 containing altered mitochondrial (ρDNA) the form circles. There are 81 ρDNA and they range from 0.05 μm to 1.6 have 1.676 . When this mini‐circular is used as marker cytoplasmic contamination, we find that purified nuclei do not contain DNA....
Mutations at the flightless-I locus (fliI) of Drosophila melanogaster cause flightlessness or, when severe, incomplete cellularization during early embryogenesis, with subsequent abnormalities in mesoderm invagination and gastrulation. After chromosome walking, deficiency mapping, transgenic analysis, we have isolated characterized cDNAs, enabling prediction complete amino acid sequence 1256-residue protein. Data base searches revealed a homologous gene Caenorhabditis elegans, corresponding...
Microdissection and microcloning of the euchromatin-heterochromatin transition region Drosophila melanogaster polytene X chromosome part euchromatin 4 reveals that they share certain features characteristic beta-heterochromatin, which is morphologically defined as loosely textured material at bases some arms. Both are mosaics many different middle-repetitive DNA sequences interspersed with single-copy sequences. Sixty percent cloned inserts derived from division 20 about 40 subdivisions 19EF...
We have sequenced the region of DNA adjacent to and including flightless (fli) gene Drosophila melanogaster molecularly characterized four transcription units within it, which we named tweety (twe), (fli), dodo (dod), penguin (pen). performed deletion transgenic analysis determine consequences quadruple removal. Only is vital organism; simultaneous absence other three allows overriding majority individuals develop adulthood fly normally. These results are evaluated in context redundancy...
Mutations in the small optic lobes (sol) gene of Drosophila melanogaster cause specific cells to degenerate developing lobes, resulting absence certain classes columnar neurons. These neuronal defects lead alterations behavioral characteristics, particularly during flight and walking maneuvers. We have isolated wild-type sol locus by microcloning chromosomal established its genetic molecular limits. Two major transcripts 5.8 5.2 kilobases are produced from this alternative splicing present...
Certain gene mutations in Drosophila melanogaster cause sluggish motor activity. We have localized the transcription unit of sluggish-A to a 14.7-kb region at base X chromosome and cloned corresponding cDNAs. The predicted protein product has significant sequence similarity Saccharomyces cerevisiae proline oxidase (EC 1.5.99.8), mitochondrial enzyme which catalyzes first step conversion glutamate. In mutant fly, activity is reduced kinetic properties different from those wild type, providing...