A5081728024- Microbial metabolism and enzyme function
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial bioremediation and biosurfactants
- Enzyme Catalysis and Immobilization
- Orthopedic Infections and Treatments
- Orthopaedic implants and arthroplasty
- Metal complexes synthesis and properties
- Glaucoma and retinal disorders
- Organometallic Complex Synthesis and Catalysis
- Corrosion Behavior and Inhibition
- Electrochemical Analysis and Applications
- Catalysis for Biomass Conversion
- Marine Biology and Environmental Chemistry
- Electrochemical sensors and biosensors
- Radioactive element chemistry and processing
- Ionic liquids properties and applications
- Asymmetric Hydrogenation and Catalysis
- Total Knee Arthroplasty Outcomes
- Organometallic Compounds Synthesis and Characterization
- Biofuel production and bioconversion
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Analytical Chemistry and Chromatography
- Chromium effects and bioremediation
- bioluminescence and chemiluminescence research
Sheffield Hallam University
2015-2024
University of Oxford
2024
Cystic Fibrosis Trust
2021
Auritec Pharmaceuticals (United States)
2011-2017
Czech Academy of Sciences, Institute of Microbiology
2017
Harvard University
1982-2013
University of Kentucky
1987-2011
Oak Crest Institute of Science
2011
Oxford University Press (United Kingdom)
2010
Athlone Institute of Technology
2008-2010
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe synthesis, redox properties, and ligand binding of heterobinuclear transition-metal macrocyclic complexes. Measurement an apparent delocalization energy in a mixed-valent copper(I)copper(II) complexR. R. Gagne, C. L. Spiro, T. J. Smith, A. Hamann, W. Thies, D. ShiemkeCite this: Am. Chem. Soc. 1981, 103, 14, 4073–4081Publication Date (Print):July 1, 1981Publication History Published online1 May 2002Published inissue 1 July...
Novel solid acid catalysts for esterification have recently been described that are made by incomplete carbonization of carbohydrates followed sulfonation. Herein, such a 'sugar catalyst' is prepared from D-glucose and its catalytic properties structure investigated in detail. This type sugar catalyst is, the first time, applied effective production biodiesel waste oils. Our results indicate highly effective, minimally polluting re-usable suited to oils with high value.
Cholinium amino acid ionic liquids ([Ch][AA] ILs), which are wholly composed of renewable biomaterials, have recently been demonstrated to very promising properties for applications in organic synthesis and biomass pretreatment. In this work, the toxicity these ILs toward enzymes bacteria was assessed, effect anion on is discussed. The inhibitory potentials type acetylcholinesterase were weaker approximately an order magnitude than traditional IL 1-butyl-3-methylimidazolium...
Abstract Cholinium amino acids ionic liquids ([Ch][AA] ILs), a novel type of bio‐ILs that can easily be prepared from renewable biomaterials, were investigated for pretreatment rice straw by selective extraction lignin this abundant lignocellulosic biomass material. Of the eight ILs examined, most demonstrated to excellent solvents. Upon using these ILs, initial saccharification rates residues substantially improved as well extent which polysaccharides could digested (>90% cellulose and...
The nano-/microscale UiO-66-NH2 metal–organic framework (MOF) materials were successfully prepared with a uniform size of about 350–400 nm and structurally characterized. Soybean epoxide hydrolase (SEH), useful for synthesis valuable vicinal diols, was the first time efficiently immobilized onto MOF. resulting novel nano-/microbiocatalyst SEH@UiO-66-NH2 manifested high SEH loading (87.3 mg/g) enzyme activity recovery (88.0%). greatly surpassed free resepct to pH stability, thermostability,...
The high degree of similarity between the mouse and human genomes is demonstrated through analysis sequence chromosome 16 (Mmu 16), which was obtained as part a whole-genome shotgun assembly genome. genome about 10% smaller than genome, owing to lower repetitive DNA content. Comparison structure protein-coding potential Mmu with that homologous segments identifies regions conserved synteny chromosomes (Hsa) 3, 8, 12, 16, 21, 22. Gene content order are highly syntenic blocks Of 731 predicted...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBinuclear complexes of macrocyclic ligands. Electrochemical and spectral properties homobinuclear CuIICuII, CuIICuI, CuICuI species including an estimated intramolecular electron transfer rateRobert R. Gagne, Carl A. Koval, Thomas J. Smith, Marc C. CimolinoCite this: Am. Chem. Soc. 1979, 101, 16, 4571–4580Publication Date (Print):August 1, 1979Publication History Published online1 May 2002Published inissue 1 August...
Human lung tryptase, a mast cell-derived trypsin-like serine protease, has been isolated from whole human tissue obtained at autopsy. Increased yields this purification process have allowed extensive characterization of the enzyme. One critical steps in scheme is use linear heparin gradient to elute active material cellulose phosphate. Gel filtration studies 1.0 M NaCl yielded an apparent Mr = 135,000, and subsequent electrophoresis on sodium dodecyl sulfate-polyacrylamide gels demonstrated...
Methane-oxidizing bacteria are ubiquitous in the environment and globally important oxidizing potent greenhouse gas methane. It is also well recognized that they have wide potential for bioremediation of organic chlorinated pollutants, thanks to substrate ranges methane monooxygenase enzymes produce. Here we demonstrated characterized model methanotroph Methylococcus capsulatus (Bath) able bioremediate chromium(VI) pollution over a range concentrations (1.4−1000 mg L−1 Cr6+), thus extending...
Abstract A novel bagasse‐based solid acid catalyst was successfully prepared through sulfonation of incompletely carbonized bagasse. range conditions for producing the were investigated, and optimized catalyst, produced under carbonization at 648 K 0.5 h 423 15 h, showed excellent catalytic activity resulted in around 95 % yield methyl oleate. Its not only substantially greater than that niobic Amberlyst‐15, but also comparable to or superior catalysts made from pure starch glucose,...
Summary: A set of isogenic mutants Bacillus subtilis 168, insertionally inactivated in the genes encoding a number lytic enzymes and sigma factor (σ D , which controls expression autolysins) was constructed. Phenotypic analysis determined individual combined roles autolysins vegetative growth. The major B. LytC (50 kDa amidase) LytD (90 glucosaminidase), were shown to have cell separation, wall turnover, antibiotic-induced lysis motility. also role general induced by sodium azide. Renaturing...
Vancomycin is commonly added to acrylic bone cement during revision arthroplasty surgery. Proprietary preparations containing vancomycin are available, but significantly more expensive. We investigated whether the elution of antibiotic from 'home-made' was comparable with expensive commercially available impregnated cement.A total 18 discs either proprietary CopalG+V; or CopalR+G by hand, were made. Each disc contained same amount (0.5 g gentamycin, 2 vancomycin) and immersed in ammonium...
In this work, we report on the development of an electrochemical biosensor for high selectivity and rapid detection Hg2+ Pb2+ ions using DNA-based specific aptamer probes labeled with ferrocene (or methylene blue) thiol groups at their 5′ 3′ termini, respectively. Aptamers were immobilized onto surface screen-printed gold electrodes via SH (thiol) groups, then cyclic voltammetry impedance spectra measurements performed in buffer solutions addition HgCl2 PbCl2 salts different concentrations....
The aim of this study was to develop a single-layer hybrid organic-inorganic sol-gel coating that is capable controlled antibiotic release for cementless hydroxyapatite (HA)-coated titanium orthopaedic prostheses.Coatings containing gentamicin at concentration 1.25% weight/volume (wt/vol), similar found in commercially available antibiotic-loaded bone cement, were prepared and tested the laboratory for: kinetics release; activity against planktonic biofilm bacterial cultures;...
A new sucralose-derived solid acid catalyst (SUCRA-SO3H), containing -Cl and -SO3H functional groups, has been shown to be highly effective for hydrolyzing β-1,4-glucans, completely cellobiose (1) into glucose (2) in 3 h converting the microcrystalline cellulose pretreated by ionic liquid with a yield of around 55% selectivity 98% within 24 at relatively moderate temperature (393K). The enhanced adsorption capacity that glucan virtue presence chloride groups act as cellulose-binding sites...
Uropathogenic Escherichia coli (UPEC) is a frequent cause of catheter-associated urinary tract infection (CAUTI). Biocides have been incorporated into catheter coatings to inhibit bacterial colonization while, ideally, exhibiting low cytotoxicity and mitigating the selection resistant populations. We compared effects long-term biocide exposure on susceptibility, biofilm formation, relative pathogenicity in eight UPEC isolates. MICs, minimum bactericidal concentrations (MBCs), eradication...
Methane-oxidizing bacteria are well known for their role in the global methane cycle and potential microbial transformation of wide range hydrocarbon chlorinated pollution. Recently, it has also emerged that methane-oxidizing interact with inorganic pollutants environment. Here, we report what believe to be first study interaction pure strains selenite. Results indicate commonly used laboratory model bacteria, Methylococcus capsulatus (Bath) Methylosinus trichosporium OB3b, both able reduce...
Tantalum (Ta) trabecular metal components are increasingly used to reconstruct major bone defects in revision arthroplasty surgery. It is known that some metals such as silver have antibacterial properties. Recent reports raised the question regarding whether Ta protective against infection This laboratory study aimed establish has intrinsic properties planktonic bacteria, or ability inhibit biofilm formation.Equal-sized pieces of and titanium (Ti) acetabular were sterilised incubated with a...