A5074178578- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Plant and animal studies
- Neurobiology and Insect Physiology Research
- Biofuel production and bioconversion
- Epigenetics and DNA Methylation
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Enzyme Production and Characterization
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- Retinal Development and Disorders
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Yeasts and Rust Fungi Studies
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Signaling Pathways in Disease
- Fermentation and Sensory Analysis
- Insect-Plant Interactions and Control
- Food Quality and Safety Studies
Australian National University
2015-2024
Google (United States)
2016-2017
May Institute
2010
In-Q-Tel
2010
Australian Research Council
2006-2007
Institut d'Investigació Biomédica de Bellvitge
2006
University of Illinois Urbana-Champaign
2006
Texas A&M University
2006
The University of Sydney
2006
Institut Català d'Oncologia
2006
Fertile queens and sterile workers are alternative forms of the adult female honeybee that develop from genetically identical larvae following differential feeding with royal jelly. We show silencing expression DNA methyltransferase Dnmt3, a key driver epigenetic global reprogramming, in newly hatched led to jelly–like effect on larval developmental trajectory; majority Dnmt3 small interfering RNA–treated individuals emerged as fully developed ovaries. Our results suggest methylation Apis is...
Parasitoid Wasp Genomes wasps, which prey on and reproduce in host insect species, play important roles plant herbivore interactions, may provide valuable tools the biological control of pest species. The Nasonia Genome Working Group (p. 343 ; see news story by Pennisi ) presents genome three very closely related species: vitripennis, N. giraulti , longicornis . findings document rapid evolution between a endosymbiont that can cause nuclear-cytoplasmic incompatibilities affect speciation.
In honey bees (Apis mellifera) the behaviorally and reproductively distinct queen worker female castes derive from same genome as a result of differential intake royal jelly are implemented in concert with DNA methylation. To determine if these very different diet-controlled phenotypes correlate unique brain methylomes, we conducted study to methyl cytosine (mC) distribution brains queens workers at single-base-pair resolution using shotgun bisulfite sequencing technology. The whole-genome...
The first generation of genome sequence assemblies and annotations have had a significant impact upon our understanding the biology sequenced species, phylogenetic relationships among study populations within across informed humans. As only few Metazoan genomes are approaching finished quality (human, mouse, fly worm), there is room for improvement most assemblies. honey bee (Apis mellifera) genome, published in 2006, was noted its bimodal GC content distribution that affected assembly some...
In honey bees ( Apis mellifera ), the development of a larva into either queen or worker depends on differential feeding with royal jelly and involves epigenomic modifications by DNA methyltransferases. To understand role methylation in this process we sequenced larval methylomes both queens workers. We show that number differentially methylated genes (DMGs) head is significantly increased relative to adult brain (2,399 vs. 560) more than 80% DMGs up-methylated larvae. Several highly...
DNA methylation systems are well characterized in vertebrates, but Drosophila melanogaster and other invertebrates remains controversial. Using the recently sequenced honey bee genome, we present a bioinformatic, molecular, biochemical characterization of functional system an insect. We report on catalytically active orthologs vertebrate methyltransferases Dnmt1 Dnmt3a b, two isoforms that contain methyl-DNA binding domain, genomic 5-methyl-deoxycytosine, CpG-methylated genes. The provides...
The remarkable olfactory power of insect species is thought to be generated by a combinatorial action two large protein families, G protein-coupled receptors (ORs) and odorant binding proteins (OBPs). In sensilla, OBPs deliver hydrophobic airborne molecules ORs, but their expression in nonolfactory tissues suggests that they also may function as general carriers other developmental physiological processes. Here we used bioinformatic experimental approaches characterize the OBP-like gene...
The genomic architecture underlying the evolution of insect social behavior is largely a mystery. Eusociality, defined by overlapping generations, parental brood care, and reproductive division labor, has most commonly evolved in Hymenopteran insects, including honey bee Apis mellifera . In this species, Major Royal Jelly Protein (MRJP) family required for all major aspects eusocial behavior. Here, using data obtained from A. genome sequencing project, we demonstrate that MRJP encoded nine...
Abstract Background In honeybees, differential feeding of female larvae promotes the occurrence two different phenotypes, a queen and worker, from identical genotypes, through incremental alterations, which affect general growth, character state alterations that result in presence or absence specific structures. Although previous studies revealed link between expression physiometabolic genes, molecular changes accompanying remain unknown. Results By using cDNA microarray analyses >6,000...
SummaryFrom studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With genome sequence hand, much improved genetic tools, are now an even more appealing target answering the major questions evolutionary population structure, social organization. At same time, agricultural incentives to understand how fall prey disease, or evade survive their pests pathogens,...
Honey bees communicate the location and desirability of valuable forage sites to their nestmates through an elaborate, symbolic “dance language.” The dance language is a uniquely complex communication system in invertebrates, neural mechanisms that generate dances are largely unknown. Here we show treatments with controlled doses biogenic amine neuromodulator octopamine selectively increased reporting resource value by forager bees. Oral topical modulated aspects related profitability...
Abstract Background Epigenetic modification of DNA via methylation is one the key inventions in eukaryotic evolution. It provides a source for switching gene activities, maintenance stable phenotypes and integration environmental genomic signals. Although this process widespread among eukaryotes, both patterns their relevant biological roles not only vary noticeably different lineages, but often are poorly understood. In addition, evolutionary origins multicellular organisms remain...
AbstractThe discovery of a family highly conserved DNA cytosine methylases in honey bees and other insects suggests that, like mammals, invertebrates possess mechanism for storing epigenetic information that controls heritable states gene expression. Recent data also show silencing methylation young larvae mimics the effects nutrition on early developmental processes determine reproductive fate bee females. We evaluate impact these findings future studies environmentally-driven phenotypic...
The cellular mechanisms employed by some organisms to produce contrasting morphological and reproductive phenotypes from the same genome remains one of key unresolved issues in biology. Honeybees (Apis mellifera) use differential feeding a haplodiploid sex determination system generate three distinct organismal outcomes genome. Here we investigate honeybee female male caste-specific microRNA transcriptomic molecular signatures during critical time larval development. Both previously...
The capacity of the honey bee to produce three phenotypically distinct organisms (two female castes; queens and sterile workers, haploid male drones) from one genotype represents most remarkable examples developmental plasticity in any phylum. queen–worker morphological reproductive divide is environmentally controlled during post-embryonic development by differential feeding. Previous studies implicated metabolic flux acting via epigenetic regulation, particular DNA methylation microRNAs,...
Abstract Carbohydrate‐metabolizing enzymes may have particularly interesting roles in the honey bee, Apis mellifera , because this social insect has an extremely carbohydrate‐rich diet, and nutrition plays important caste determination socially mediated behavioural plasticity. We annotated a total of 174 genes encoding carbohydrate‐metabolizing 28 lipid‐metabolizing enzymes, based on orthology to their counterparts fly, Drosophila melanogaster, mosquito, Anopheles gambiae . found that number...
Abstract The cyanobacterium Synechocystis sp. strain PCC6803 possesses three modes of inorganic carbon (Ci) uptake that are inducible under Ci stress and dramatically enhance the efficiency CO2-concentrating mechanism (CCM). effects limitation on mRNA transcript abundance these systems physiological expression CCM were investigated in detail this cyanobacterium. Transcript was assessed with semiquantitative real-time reverse transcriptase-polymerase chain reaction techniques. Cells aerated...