A5022565043- Sepsis Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Multiple Myeloma Research and Treatments
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Machine Learning in Healthcare
- Cardiac Arrest and Resuscitation
- Emergency and Acute Care Studies
- Trauma and Emergency Care Studies
- Cell death mechanisms and regulation
- Peptidase Inhibition and Analysis
- Respiratory Support and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Topic Modeling
- Acute Ischemic Stroke Management
- Protein Degradation and Inhibitors
- Cardiac, Anesthesia and Surgical Outcomes
- Hemodynamic Monitoring and Therapy
- Bacterial Identification and Susceptibility Testing
- Ubiquitin and proteasome pathways
- Nuclear Structure and Function
- Non-Invasive Vital Sign Monitoring
- Neuroblastoma Research and Treatments
- Clinical Reasoning and Diagnostic Skills
- Opioid Use Disorder Treatment
University of Wisconsin–Madison
2022-2025
University of Chicago
2014-2023
Society of Critical Care Medicine
2022
Ottawa University
2022
Anna Needs Neuroblastoma Answers
2020
Christus Health
2019
Molecular Oncology (United States)
2018
University of California, Los Angeles
2018
Lurie Children's Hospital
2017
Indiana University Bloomington
2010-2014
Parasitoid Wasp Genomes wasps, which prey on and reproduce in host insect species, play important roles plant herbivore interactions, may provide valuable tools the biological control of pest species. The Nasonia Genome Working Group (p. 343 ; see news story by Pennisi ) presents genome three very closely related species: vitripennis, N. giraulti , longicornis . findings document rapid evolution between a endosymbiont that can cause nuclear-cytoplasmic incompatibilities affect speciation.
Abstract Background Data generated from liquid chromatography coupled to high-resolution mass spectrometry (LC-MS)-based studies of a biological sample can contain large amounts biologically significant information in the form proteins, peptides, and metabolites. Interpreting this data involves inferring masses abundances biomolecules injected into instrument. Because inherent complexity spectral patterns produced by these biomolecules, analysis is significantly enhanced using visualization...
Multisubunit RNA polymerases IV and V (Pols V) mediate RNA-directed DNA methylation transcriptional silencing of retrotransposons heterochromatic repeats in plants. We identified genomic sites Pol occupancy parallel with siRNA deep sequencing methylcytosine mapping, comparing wild-type plants mutants defective for IV, V, or both Pols V. Approximately 60% V-associated regions encompass 24-nucleotide (nt) complementarity cytosine methylation, consistent being guided by base-pairing...
Abstract Summary: DeconMSn accurately determines the monoisotopic mass and charge state of parent ions from high-resolution tandem spectrometry data, offering significant improvement for LTQ_FT LTQ_Orbitrap instruments over commercially delivered Thermo Fisher Scientific's extract_msn tool. Optimal ion tolerance values can be determined using accurate information, thus improving peptide identifications high-mass measurement accuracy experiments. For low-resolution data LCQ LTQ instruments,...
Importance The US heart allocation system prioritizes medically urgent candidates with a high risk of dying without transplant. current therapy-based 6-status is susceptible to manipulation and has limited rank ordering ability. Objective To develop validate candidate score that incorporates clinical, laboratory, hemodynamic data. Design, Setting, Participants A registry-based observational study adult transplant (aged ≥18 years) from the listed between January 1, 2019, December 31, 2022,...
A high-throughput approach and platform using 15 min reversed-phase capillary liquid chromatography (RPLC) separations in conjunction with ion mobility spectrometry-mass spectrometry (IMS-MS) measurements was evaluated for the rapid analysis of complex proteomics samples. To test separation quality short LC gradient, a sample prepared by spiking 20 reference peptides at varying concentrations from 1 ng/mL to 10 microg/mL into tryptic digest mouse blood plasma analyzed both LC-Linear Ion Trap...
Glycosylation is an important protein modification that involves enzymatic attachment of sugars to amino acid residues. Understanding the structure these and effects glycosylation are vital for developing indicators disease development progression. Although computational methods based on mass spectrometric data have proven be effective in monitoring changes glycome, such glycoproteome challenging, largely due inherent complexity simultaneously studying glycan structures with their...
Protein glycosylation is one of the most common post‐translational modifications, estimated to occur in over 50% human proteins. Mass spectrometry (MS)‐based approaches involving different fragmentation mechanisms have been frequently used detect and characterize protein N‐linked glycosylations. In addition popular Collision‐Induced Dissociation (CID), high‐energy C‐trap dissociation (HCD) fragmentation, which a feature linear ion trap orbitrap hybrid mass spectrometer (LTQ Orbitrap), has...
Objectives: To describe the deployment of pediatric Calculated Assessment Risk and Triage (pCART), a machine learning (ML) model to predict risk direct ward ICU transfer within 12 hours, associated improved outcomes among hospitalized children. Design: Pre- vs. post-implementation study. Setting: An urban, tertiary-care, academic hospital. Patients: Pediatric (age < 18 yr) admissions from May 1, 2019, April 30, 2023. Interventions: None. Measurements Main Results: Patients were divided...
Objective: Implementing machine learning models to identify clinical deterioration on the wards is associated with improved outcomes. However, these have high false positive rates and only use structured data. Therefore, we aim compare without information from notes for predicting deterioration. Materials Methods: Adults admitted at University of Chicago (development cohort) Wisconsin-Madison (external validation were included. Predictors consisted unstructured variables extracted as Concept...
Objective: Overdose Fatality Review (OFR) is a public health process in which cases of fatal overdose are carefully reviewed to identify prevention strategies. Current OFR requires review multiple unconnected data sources, manually intensive process. We aimed use human factors design principles develop comprehensive dashboard that could facilitate enhanced processes support OFR. Materials and Methods: first surveyed leaders Wisconsin using the NASA Task Load Index (NASA-TLX) conducted...
<sec> <title>BACKGROUND</title> Implementing machine learning models to identify clinical deterioration on the wards is associated with improved outcomes. However, these have high false positive rates and only use structured data. </sec> <title>OBJECTIVE</title> We aim compare without information from notes for predicting deterioration. <title>METHODS</title> Adults admitted at University of Chicago (development cohort) Wisconsin-Madison (external validation were included. Predictors...
We investigated the combination of weak anion exchange (WAX) fractionation and on-line reversed-phase liquid chromatography (RPLC) separation using a 12 T FTICR mass spectrometer for detection intact proteins from Shewanella oneidensis MR-1 cell lysate. This work aimed at optimizing protein profiling level that incorporates their modification state. A total 715 were detected, combined results WAX fractions unfractionated lysate aligned LC-MS features to facilitate abundance measurements....
As a common post-translational modification, protein glycosylation plays an important role in many biological processes, and it is known to be associated with human diseases. Mass spectrometry (MS)-based glycomic profiling techniques have been developed measure the abundances of glycans complex samples applied discovery putative glycan biomarkers. To automate annotation profiles liquid chromatography-MS (LC-MS) data, we present here user-friendly software tool, MultiGlycan, implemented C# on...
Prostate specific antigen (PSA) is currently used as a biomarker to diagnose prostate cancer. PSA testing has been widely detect and screen However, in the diagnostic gray zone, test does not clearly distinguish between benign hypertrophy cancer due their overlap. To develop more sensitive candidate biomarkers for cancer, an in-depth understanding of biochemical characteristics (such glycosylation) needed. single glycosylation site at Asn69, with glycans constituting approximately 8% protein...
Exportin1 (XPO1; also known as chromosome maintenance region 1, or CRM1) controls nucleo-cytoplasmic transport of most tumor suppressors and is overexpressed in many cancers, including multiple myeloma, functionally impairing suppressive function via target mislocalization. Selective inhibitor nuclear export (SINE) compounds block XPO1-mediated escape by disrupting cargo protein binding, leading to retention suppressors, induction cancer cell death, sensitization other drugs. Combined...