A5088187016- Peanut Plant Research Studies
- Coconut Research and Applications
- Agricultural pest management studies
- Plant Virus Research Studies
- Nitrogen and Sulfur Effects on Brassica
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Entomopathogenic Microorganisms in Pest Control
- Plant Physiology and Cultivation Studies
- Plant and Fungal Interactions Research
- Insect and Arachnid Ecology and Behavior
- Plant Molecular Biology Research
- Agronomic Practices and Intercropping Systems
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Spectroscopy and Chemometric Analyses
- Insect and Pesticide Research
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Soybean genetics and cultivation
- Mycotoxins in Agriculture and Food
- Fermentation and Sensory Analysis
Northwest A&F University
2025
National Peanut Research Laboratory
2015-2024
Agricultural Research Service
2006-2023
United States Department of Agriculture
2003-2023
Auburn University
2017
U.S. Horticultural Research Laboratory
2003-2006
Appalachian Fruit Research Laboratory
2002
Parasitoid Wasp Genomes wasps, which prey on and reproduce in host insect species, play important roles plant herbivore interactions, may provide valuable tools the biological control of pest species. The Nasonia Genome Working Group (p. 343 ; see news story by Pennisi ) presents genome three very closely related species: vitripennis, N. giraulti , longicornis . findings document rapid evolution between a endosymbiont that can cause nuclear-cytoplasmic incompatibilities affect speciation.
Abstract Aphids are the leading pests in agricultural crops. A large-scale sequencing of 40,904 ESTs from pea aphid Acyrthosiphon pisum was carried out to define a catalog 12,082 unique transcripts. strong AT bias found, indicating compositional shift between Drosophila melanogaster and A. . An silico profiling analysis characterized 135 transcripts specific pea-aphid tissues (relating bacteriocytes parthenogenetic embryos). This project is first address genetics Hemiptera hemimetabolous insect.
Peanut (Arachis hypogaea L.) is an important crop economically and nutritionally, one of the most susceptible host crops to colonization Aspergillus parasiticus subsequent aflatoxin contamination. Knowledge from molecular genetic studies could help devise strategies in alleviating this problem; however, few peanut DNA sequences are available public database. In order understand basis resistance contamination, a large-scale project was conducted generate expressed sequence tags (ESTs)...
Peanut (Arachis hypogaea L.) is a grain legume crop that originated from South America and now grown around the world. growth habit affects variety's adaptability, planting patterns, mechanized harvesting, disease resistance, yield. The objective of this study was to map quantitative trait locus (QTL) associated with peanut habit-related traits by combining genome-wide association analysis (GWAS) bulked segregant sequencing (BSA-seq) methods.GWAS performed 17,223 single nucleotide...
Expressed sequence tag (EST) libraries for cultivated peanut (Arachis hypogaea L.) were developed from two cDNA constructed by means of mRNA prepared leaves line C34-24 (resistant to leaf spots and Tomato spotted wilt virus) immature pods A13 (tolerant drought stress preharvest aflatoxin contamination). Randomly selected clones partially sequenced generate a total 1825 ESTs, 769 the library 1056 library, in which 536 unique ESTs identified, respectively. Results BLASTx search showed that...
Late leaf spot disease caused by Cercosporidium personatum is one of the most destructive foliar diseases peanut (Arachis hypogaea) worldwide. The objective this research was to identify resistance genes in response using microarray and real-time polymerase chain reaction (PCR). To transcripts involved resistance, we studied gene expression profiles two genotypes, resistant or susceptible disease, cDNA containing 384 unigenes selected from expressed sequenced tag (EST) libraries challenged...
The Food and Agriculture Organization of the United Nations estimates that 25% food crops in world are contaminated with aflatoxins. That represents 100 million tons being destroyed or diverted to non-human consumption each year. Aflatoxins powerful carcinogens normally accumulated by fungi Aspergillus flavus A. parasiticus cereals, nuts, root other agricultural products. Silencing five aflatoxin-synthesis genes RNA interference (RNAi) peanut plants was used control aflatoxin accumulation...
Peanut is vulnerable to a range of foliar diseases such as spotted wilt caused by Tomato virus (TSWV), early ( Cercospora arachidicola ) and late Cercosporidium personatum leaf spots, southern stem rot Sclerotium rolfsii ), sclerotinia blight Sclerotinia minor ). In this study, we report the generation 17,376 peanut expressed sequence tags (ESTs) from tissues cultivar (Tifrunner, resistant TSWV spots) breeding line (GT‐C20, susceptible spots). After trimming vector discarding low quality...
Grape expressed sequence tags (ESTs) are a new resource for developing simple repeat (SSR) functional markers genotyping and genetic mapping. An integrated pipeline including several computational tools SSR identification annotation was developed to identify 6,447 EST-SSR sequences from total collection of 215,609 grape ESTs retrieved NCBI. The EST-SSRs were further reduced 1,701 non-redundant via clustering analysis, 1,037 them successfully designed with primer pairs flanking the motifs....
Peanut flavor is a complex and important trait affected by raw material processing technology owing to its significant impact on consumer preference. In this research, principal component analysis (PCA) 33 representative traits associated with revealed that total sugars, sucrose, tocopherols provided more information related peanut flavor. Genome-wide association studies (GWAS) using 102 U.S. mini-core accessions were performed study associations between 12,526 single nucleotide polymorphic...
Abstract Agronomic and physiological traits in peanut ( Arachis hypogaea ) are important to breeders for selecting high‐yielding resilient genotypes. However, direct measurement of these is labor‐intensive time‐consuming. This study assessed the feasibility using unmanned aerial vehicles (UAV)‐based hyperspectral imaging machine learning (ML) techniques predict three agronomic (biomass, pod count, yield) two (photosynthesis stomatal conductance) under drought stress. Two different approaches...
Abstract We report three novel small RNA viruses uncovered from cDNA libraries parasitoid wasps in the genus Nasonia . The genome of this kind virus is a positive‐sense single‐stranded with 3′ poly(A), which facilitates cloning cDNAs. Two viruses, NvitV‐1 and NvitV‐2, possess RNA‐dependent polymerase that associates them family Iflaviridae order Picornavirales A third virus, NvitV‐3, most similar to Nora Drosophila reverse transcription‐PCR method developed for indicates it persistent...
The level of oleic acid in peanut seed is one the most important factors determining quality and controlled by two pairs homeologous genes ( FAD2A FAD2B). genotypes eight F8 breeding lines were determined as AABB, aaBB, AAbb, aabb real-time polymerase chain reaction sequencing. Fresh seeds collected from five developmental stages and, after drying, used for chemical analysis. Our results showed that (1) developed, weight, oil content, significantly increased, whereas four other fatty levels...
Peanut is an important legume providing products with nutrient dense including mineral nutrition. However, the genetic basis underlying variations in composition peanut still unknown. Genome-wide association studies (GWAS) of concentrations 13 elements combined coexpression network were performed using a diverse panel 120 genotypes mainly selected from U.S. mini core collection. A total 36 significant quantitative trait loci (QTLs) associated five elemental identified phenotypic variation...
Abstract Peanut ( Arachis Hypogaea L) is an economic cash crop mainly planted in arid and semi‐arid regions where drought causes approximately 20% yield losses every year. It has been demonstrated that tolerance can be achieved by two different mechanisms plants: either saving water or extracting more from the soil (water spender genotypes). The objective of this research was to screen for these peanut. Plants were grown rainout shelters under irrigated conditions until mid‐pod filling, then...
Many plant ESTs have been sequenced as an alternative to whole genome sequences, including peanut because of the size and complexity. The US research community had historic 2004 Atlanta Genomics Workshop named EST project a main priority. As August 2011, deposited 252,832 in public NCBI database, this resource has providing valuable tools core foundations for various genome-scale experiments before sequencing project. These resources used marker development, gene cloning, microarray...
Drought can significantly limit yield and quality in peanut (Arachis hypogaea L.), depending on its timing, duration severity. The objective of this study was to identify potential molecular mechanism(s) utilising a candidate-gene approach five genotypes with contrasting drought responses. An early season stress treatment applied under environmentally controlled rain-out shelters. When water completely withheld for 3 weeks, no physical differences were observed treated plants compared their...