A5052879526- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Peanut Plant Research Studies
- Nematode management and characterization studies
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant pathogens and resistance mechanisms
- Lipid metabolism and biosynthesis
- Plant Genetic and Mutation Studies
- Coconut Research and Applications
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Cassava research and cyanide
- Nitrogen and Sulfur Effects on Brassica
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Agricultural pest management studies
- Plant Reproductive Biology
- Biofuel production and bioconversion
- Seed Germination and Physiology
Donald Danforth Plant Science Center
2014-2024
United States Department of Agriculture
2006-2022
Agricultural Research Service - Midwest Area
2020-2022
Agricultural Research Service
2006-2021
Seed protein, oil content and yield are highly correlated agronomically important traits that essentially account for the economic value of soybean. The underlying molecular mechanisms selection these seed during soybean domestication are, however, less known. Here, we demonstrate a CCT gene, POWR1, underlies large-effect protein/oil QTL. A causative TE insertion truncates its domain substantially increases content, weight, while decreasing protein content. POWR1 pleiotropically controls...
Abstract Seed development is programmed by expression of many genes in plants. maturation an important developmental process to soybean seed quality and yield. DNA methylation a major epigenetic modification regulating gene expression. However, little known about the dynamic nature its effects on during plant development. Through whole-genome bisulfite sequencing, we showed that went through changes maturation. An average 66% CG, 45% CHG 9% CHH contexts was methylated cotyledons. levels...
Increasing seed oil content is one of the most important breeding goals for soybean due to a high global demand edible vegetable oil. However, genetic improvement has been difficult in because complexity metabolism. Determining major variants and molecular mechanisms conferring accumulation critical substantial enhancement other oilseed crops. In this study, we evaluated contents 219 diverse accessions across six different environments dissected underlying mechanism using high-resolution...
Soybean [ Glycine max (L.) Merr.] was domesticated from wild soybean ( G . soja Sieb. and Zucc.) has been further improved as a dual-use seed crop to provide highly valuable oil protein for food, feed, industrial applications. However, the underlying genetic molecular basis remains less understood. Having combined high-confidence bi-parental linkage mapping with high-resolution association analysis based on 631 whole sequenced genomes, we mapped major QTLs chromosome15 sugar transporter gene...
Variation in seed oil composition and content among soybean varieties is largely attributed to differences transcript sequences and/or accumulation of production related genes seeds. Discovery analysis sequence expression variations these will accelerate quality improvement.In an effort identify variations, we sequenced the transcriptomes seeds from nine lines varying total content. Our results showed that 69,338 distinct transcripts 32,885 annotated were expressed A 8,037 polymorphisms...
With advances in next-generation sequencing technologies, an unprecedented amount of soybean accessions has been sequenced by many individual studies and made available as raw reads for post-genomic research.To develop a consolidated user-friendly genomic resource research, we the resequencing data 1465 genomes public 91 highly diverse wild newly sequenced. These altogether provided collection 1556 1501 (1.5 K). The comprises wild, landraces elite cultivars that were grown East Asia or major...
North American soybean breeders have successfully developed a large number of elite cultivars with diverse maturity groups (MG) from small ancestral landraces. To understand molecular and genetic basis underlying the variation in their flowering times, we integrated pedigree data 166 representing breeding. Network analysis visualization relationships revealed clear separation southern northern breeding programs, suggesting that little exchange occurred between (MG 0–IV) V–VIII). We also...
Glutathione peroxidases (GPXs) protect cells against damage caused by reactive oxygen species (ROS) and play key roles in regulating many biological processes. Here, five GPXs were identified the Ricinus communis genome. Phylogenetic analysis displayed that categorized into groups. Conserved domain gene structure analyses showed from different plant harbored four highly similar motifs conserved exon-intron arrangement patterns, indicating their function may have been during evolution....
Cereal aleurone has been established as a model system to investigate giberrellin (GA) and abscisic acid (ABA) responses. Using Barley 1 GeneChip, we examined the mRNA accumulation of over 22 000 genes in de-embryonated barley treated with GA ABA. We observed that 328 had more than threefold change response treatment, whereas 206 ABA treatment. Interestingly, approximately 2.5-fold were up-regulated down-regulated by Eighty-three differentially regulated both Most subject antagonistic...
The level of oleic acid in peanut seed is one the most important factors determining quality and controlled by two pairs homeologous genes ( FAD2A FAD2B). genotypes eight F8 breeding lines were determined as AABB, aaBB, AAbb, aabb real-time polymerase chain reaction sequencing. Fresh seeds collected from five developmental stages and, after drying, used for chemical analysis. Our results showed that (1) developed, weight, oil content, significantly increased, whereas four other fatty levels...
Seeds are the economic basis of oilseed crops, especially soybeans, most widely cultivated crop worldwide. Seed development is accompanied by a multitude diverse cellular processes, and revealing underlying regulatory activities critical for seed improvement.In this study, we profiled transcriptomes developing seeds at 20, 25, 30, 40 days after flowering (DAF), as these stages represent time points from early to full development. We identified set highly abundant genes highlighted importance...
Identification and characterization of a 254-kb genomic deletion on duplicated chromosome segment that resulted in low level palmitic acid soybean seeds using transcriptome sequencing. A large number genotypes varying seed oil composition content have been identified. Understanding the molecular mechanisms underlying these variations is important for breeders to effectively utilize them as genetic resource. Through design application bioinformatics approach, we identified nine co-regulated...
Cis-regulatory elements (CREs) are integral to the spatiotemporal and quantitative expression dynamics of target genes, thus directly influencing phenotypic variation evolution. However, many these CREs become highly susceptible transcriptional silencing when in a transgenic state, particularly organised as tandem repeats. We investigated mechanism this phenomenon found that three six selected flower-specific were prone context. determined was caused by ectopic non-coding RNAs (ncRNAs),...
This strategic plan summarizes the major accomplishments achieved in last quinquennial by soybean [Glycine max (L.) Merr.] genetics and genomics research community outlines key priorities for next 5 years (2024-2028). work is result of deliberations among over 50 researchers during a 2-day workshop St Louis, MO, USA, at end 2022. The divided into seven traditional areas/disciplines: Breeding, Biotic Interactions, Physiology Abiotic Stress, Functional Genomics, Biotechnology, Genomic...
Abstract Screening a transposon‐mutagenized soybean population led to the discovery of recessively inherited chlorotic phenotype. This “y24” phenotype results in smaller stature, weaker stems, and root system. Genome sequencing identified 15 candidate genes with mutations likely result loss function. Amplicon segregating was then used narrow list single mutation, single‐base change Glyma.07G102300 that disrupts splicing second intron. Single cell transcriptomic profiling indicates this gene...
Abstract Screening a transposon-mutagenized soybean population led to the discovery of recessively inherited chlorotic phenotype. This “vir1” phenotype results in smaller stature, weaker stems, and root system with nodules. Genome sequencing identified 15 candidate genes mutations likely result loss function. Amplicon segregating was then used narrow list single mutation, single-base change Glyma.07G102300 that disrupts splicing second intron. Single cell transcriptomic profiling indicates...
Summary With the advance of next-generation sequencing technologies, over 15 terabytes raw soybean genome data were generated and made available in public. To develop a consolidated, diverse, user-friendly genomic resource to facilitate post-genomic research, we sequenced 91 highly diverse wild genomes representing entire US collection accessions increase genetic diversity genomes. Having integrated analyzed with public data, identified annotated 32 million single nucleotide polymorphisms...
ABSTRACT Some crops inhibit shoot growth but maintain root under water-deficit conditions. Unraveling the molecular mechanisms of plasticity water deficit conditions in plants remains a major challenge. We developed an efficient platform for identifying core transcription factors (TFs) that collectively regulate each other and/or themselves response to stress, and exploring their interconnected regulatory circuitry involved maintenance soybean. performed multi-species phylogenetic...
Abstract Background: Seeds are the economic basis of oilseed crops, especially for soybean, thus far most widely cultivated crop worldwide. Seed development is accompanied with a multitude diverse cellular processes and revealing underlying regulatory activities critical seed improvement. Results: Here, we profiled transcriptomes developing seeds (20, 25, 30, 40 days after flowering) representing key points from early to full development. We identified set highly-abundant genes highlighted...