A5012574725- Plant tissue culture and regeneration
- Soybean genetics and cultivation
- Lipid metabolism and biosynthesis
- Transgenic Plants and Applications
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Legume Nitrogen Fixing Symbiosis
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
- Biodiesel Production and Applications
- Biofuel production and bioconversion
- Plant responses to elevated CO2
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Food composition and properties
- HIV Research and Treatment
- Plant Water Relations and Carbon Dynamics
- HIV/AIDS drug development and treatment
- Nitrogen and Sulfur Effects on Brassica
- Genetically Modified Organisms Research
- Coconut Research and Applications
University of Nebraska–Lincoln
2016-2025
Metabolon (United States)
2012
Sorbonne Université
2012
Cornell University
2012
Institut de Biologie Physico-Chimique
2012
Colegio de Postgraduados
2012
South Valley University
2001
Sohag University
2001
United States Department of Agriculture
2001
Meta-analyses and theory show that with rising atmospheric [CO 2 ], Rubisco has become the greatest limitation to light-saturated leaf CO assimilation rates ( A sat ) in C 4 crops. So would transgenically increasing increase result increased productivity field? Here, we successfully overexpressed small subunit RbcS accumulation factor 1 Raf1 both sorghum sugarcane, resulting significant increases content of 13 25% up 90% respectively. 12 15% enzyme activity ~40% three independent transgenic...
An emerging topic in plant biology is whether plants display analogous elements of mammalian programmed cell death during development and defense against pathogen attack. In many plant-pathogen interactions, occurs both susceptible resistant host responses. For example, specific recognition responses trigger formation the hypersensitive response activation mechanisms, resulting restriction growth disease development. Several studies indicate that involves a plant-encoded pathway for death....
Phenotypic characterization of soybean event 335-13, which possesses oil with an increased oleic acid content (> 85%) and reduced palmitic (< 5%), was conducted across multiple environments during 2004 2005. Under these conditions, the stability novel fatty profile not influenced by environment. Importantly, 335-13 compromised in yield both irrigated non-irrigated production schemes. Moreover, seed characteristics, including total protein, as well amino profile, were altered a result large...
Abstract Stable transformation of plants is a powerful tool for hypothesis testing. A rapid and reliable evaluation method the transgenic allele copy number homozygosity vital in analysing these transformations. Here suitability Southern blot analysis, thermal asymmetric interlaced (TAIL‐)PCR, quantitative (q)PCR digital droplet (dd)PCR to estimate T‐DNA number, locus complexity were compared tobacco. analysis ddPCR on three generations offspring with contrasting zygosity entirely...
Predictions suggest that current crop production needs to double by 2050 meet global food and energy demands. Based on theory experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected enhance C3 photosynthesis yields. Here we test how expression cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation seed yield (SY) in a major (soybean, Glycine max). For three growing...
Soybean [Glycine max (L.) Merr.] is a unique crop species because it has high levels of both protein and oil in its seed. Of the many quantitative trait loci (QTL) controlling soybean seed content, alleles cqSeed protein-003 QTL on chromosome 20 exert greatest additive effect. The high-protein allele exists cultivated wild (Glycine soja Siebold & Zucc.) germplasm. Our objective was to fine map this enable positional-based cloning underlying causative gene(s). Fine mapping achieved by...
Summary Seed oils have proved recalcitrant to modification for the production of industrially useful lipids. Here, we demonstrate successful metabolic engineering and subsequent field an oilseed crop with highest accumulation unusual oil achieved so far in transgenic plants. Previously, expression E uonymus alatus diacylglycerol acetyltransferase ( a DA c T ) gene wild‐type A rabidopsis seeds resulted 45 mol% 3‐acetyl‐1,2‐diacyl‐ sn ‐glycerols (acetyl‐ TAG s) seed (Durrett et al ., 2010 PNAS...
Summary Losses of floral pigmentation represent one the most common evolutionary transitions in flower color, yet genetic basis for these changes has been elucidated only a handful cases. Here we used crossing studies, bulk‐segregant RNA sequencing, phylogenetic analyses and functional tests to identify gene(s) responsible transition white flowers Iochroma loxense . Crosses between I. its blue‐flowered sister species, cyaneum , suggested that single locus controls color difference allele...
Summary Central metabolism produces amino and fatty acids for protein lipids that establish seed value. Biosynthesis of storage reserves occurs in multiple organelles exchange central intermediates including two essential metabolites, malate, pyruvate are linked by malic enzyme. Malic enzyme can be active subcellular compartments, partitioning carbon reducing equivalents anabolic catabolic requirements. Prior studies based on isotopic labeling steady‐state metabolic flux analyses indicated...
Abstract Multicellular eukaryotes demonstrate nongenetic, heritable phenotypic versatility in their adaptation to environmental changes. This inclusive inheritance is composed of interacting epigenetic, maternal, and factors. Yet-unidentified maternal effects can have a pronounced influence on plant changing conditions. To explore the control phenotypy higher plants, we examined effect single nuclear gene expression transmission variability Arabidopsis (Arabidopsis thaliana). MutS HOMOLOG1...
Sorghum prolamins, termed kafirins, are categorized into subgroups α, β, and γ. The kafirins co-translationally translocated to the endoplasmic reticulum (ER) where they assembled discrete protein bodies that tend be poorly digestible with low functionality in food feed applications. As a means address issues surrounding digestibility sorghum, we employed biotechnology approach is designed alter body structure, concomitant synthesis of co-protein endosperm fraction grain. Wherein...
Nitrogen is essential for plant growth and development. Improving the ability of plants to acquire assimilate nitrogen more efficiently a key agronomic parameter that will augment sustainability in agriculture. A transcription factor approach was pursued address improvement use efficiency two major commodity crops. To this end, Zea mays Dof1 (ZmDof1) expressed both wheat (Triticum aestivum) sorghum (Sorghum bicolor) either constitutively, UBI4 promoter from sugarcane, or tissue specific...
Abstract Enhancing crop water use efficiency (WUE) is a key target trait for climatic resilience and expanding cultivation on marginal lands. Engineering lower stomatal density to reduce conductance (gs) has improved WUE in multiple C3 species. However, reducing gs species often reduces photosynthetic carbon gain. A different response expected C4 plants because they possess specialized anatomy biochemistry which concentrates CO2 at the site of fixation. This modifies relationship...
Through a single desaturation step, the Borago officinalis L. Δ 6 desaturase can convert linoleic acid and α‐linolenic to γ‐linolenic (GLA) stearidonic (STA), respectively. Both GLA STA are of interest pharmaceutical nutraceutical industries. Production these fatty acids is costly. One potential strategy reduce production cost would be generate them in major oilseed crop. To this end, cDNA B. ‐desaturase gene was cloned downstream embryo‐specific promoter β‐conglycinin. The resultant...