Guizhen Kan

ORCID: 0000-0003-3252-6106
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About
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Research Areas
  • Soybean genetics and cultivation
  • Legume Nitrogen Fixing Symbiosis
  • Plant pathogens and resistance mechanisms
  • Plant nutrient uptake and metabolism
  • Phytase and its Applications
  • Plant Stress Responses and Tolerance
  • Peanut Plant Research Studies
  • Nitrogen and Sulfur Effects on Brassica
  • Insect-Plant Interactions and Control
  • Plant Molecular Biology Research
  • Plant Parasitism and Resistance
  • Lipid metabolism and biosynthesis
  • Seed Germination and Physiology
  • Plant Micronutrient Interactions and Effects
  • Nematode management and characterization studies
  • Genetic Mapping and Diversity in Plants and Animals
  • Banana Cultivation and Research

Nanjing Agricultural University
2014-2024

Phosphorus (P) is essential for all living cells and organisms, low-P stress a major factor constraining plant growth yield worldwide. In plants, P efficiency complex quantitative trait involving multiple genes, the mechanisms underlying are largely unknown. Combining linkage analysis, genome-wide candidate-gene association analyses, transformation, we identified soybean gene related to efficiency, determined its favorable haplotypes developed valuable functional markers. First, six genomic...

10.1371/journal.pgen.1004061 article EN cc-by PLoS Genetics 2014-01-02

Increasing seed oil content is one of the most important breeding goals for soybean due to a high global demand edible vegetable oil. However, genetic improvement has been difficult in because complexity metabolism. Determining major variants and molecular mechanisms conferring accumulation critical substantial enhancement other oilseed crops. In this study, we evaluated contents 219 diverse accessions across six different environments dissected underlying mechanism using high-resolution...

10.1371/journal.pgen.1008267 article EN public-domain PLoS Genetics 2019-07-10

Salt stress is one of the major abiotic factors that affect metabolism, growth and development plants, soybean [Glycine max (L.) Merr.] germination sensitive to salt stress. Thus, ensure successful establishment productivity soybeans in saline soil, genetic mechanisms tolerance at stage need be explored. In this study, a population 184 recombinant inbred lines (RILs) was utilized map quantitative trait loci (QTLs) related tolerance. A QTL named qST-8 closely linked with marker Sat_162...

10.1371/journal.pgen.1007798 article EN cc-by PLoS Genetics 2019-01-07

Seed yield, determined mainly by seed numbers and weight, is the primary target of soybean breeding. Identifying genes underlying yield-related traits great significance. Through joint linkage mapping a genome-wide association study for 100-seed we cloned GmGA3ox1, gene encoding gibberellin 3β-hydroxylase, which key enzyme in synthesis pathway. Genome resequencing identified beneficial GmGA3ox1 haplotype contributing to high was further confirmed transformants. CRISPR/Cas9-generated gmga3ox1...

10.1111/nph.18153 article EN New Phytologist 2022-04-09

Photoperiod-sensitive plants such as soybean (Glycine max) often face threats from herbivorous insects throughout their whole growth period and especially during flowering; however, little is known about the relationship between plant flowering insect resistance. Here, we used gene editing, multiple omics, genetic diversity evolutionary analyses to confirm that calcium-dependent protein kinase GmCDPK38 plays a dual role in coordinating time regulation resistance of soybean. Haplotype 2...

10.1093/plphys/kiac260 article EN PLANT PHYSIOLOGY 2022-05-31

Wild soybean, the progenitor of cultivated is an important gene pool for ongoing soybean breeding efforts. To identify yield-enhancing quantitative trait locus (QTL) or from wild 113 soybeans accessions were phenotyped five yield-related traits and genotyped with 85 simple sequence repeat (SSR) markers to conduct association mapping. A total 892 alleles detected SSR markers, average 10.49 alleles; corresponding PIC values ranged 0.07 0.92, 0.73. The genetic diversity each marker 0.93, 0.75....

10.1270/jsbbs.63.441 article EN Breeding Science 2014-01-01

Pod dehiscence (shattering) is the main cause of serious yield loss during soybean mechanical harvesting process. A better understanding genetic architecture and molecular mechanisms pod great significance for breeding. In this study, genome-wide association analysis (GWAS) with NJAU 355K SoySNP array was performed to detect single nucleotide polymorphisms (SNPs) associated in an panel containing 211 accessions across five environments. total 163 SNPs were identified as significantly...

10.3389/fpls.2019.00811 article EN cc-by Frontiers in Plant Science 2019-06-21

Compared to β-conglycinin, glycinin contains 3-4 times the methionine and cysteine (sulfur-containing amino acids), accounting for approximately 40 30%, respectively, of total storage protein in soybean. Increasing soybean content while improving ratio β-conglycinin is great significance breeding soy food products. The objective this study analyze genetic mechanism regulating contents by using a recombinant inbred line (RIL) population derived from cross between Kefeng No. 1 Nannong 1138-2....

10.1021/acs.jafc.6b00167 article EN Journal of Agricultural and Food Chemistry 2016-04-12

ABSTRACT Increasing plant photosynthetic capacity is a promising approach to boost yields, but it particularly challenging in C 3 crops, such as soybean ( Glycine max (L.) Merr.). Here, we identified GmFtsH25 , encoding member of the filamentation temperature‐sensitive protein H protease family, major gene involved photosynthesis, using linkage mapping and genome‐wide association study. Overexpressing resulted more grana thylakoid stacks chloroplasts increased efficiency starch content,...

10.1111/jipb.13405 article EN Journal of Integrative Plant Biology 2022-11-09

Salt tolerance in soybean [Glycine max (L.) Merr.] at the seed germination stage is a critical determinant of stable stand establishment saline soil. This study examined one population 184 recombinant inbred lines (RILs, F7:11) derived from cross between Kefeng1 and Nannong1138-2 natural consisting 196 landraces. A total 11 quantitative trait loci (QTLs) 22 simple sequence repeat (SSR) associated with three salt indices were detected by linkage association mapping. The SSR marker Sat_162 was...

10.1270/jsbbs.15147 article EN Breeding Science 2016-01-01

Abstract Excess salinity is a major environmental threat to agriculture. However, the adaptive mechanisms underlying salt tolerance in soybean ( Glycine max [L.] Merr.) remain unclear. The aim of this study was investigate differences between salt‐tolerant and salt‐sensitive soybeans their physiological characteristics expression patterns 8 cation antiporters under stress. inhibitory effects stress on growth photosynthesis were stronger than those soybean. concentration phosphate most other...

10.1002/ldr.3005 article EN Land Degradation and Development 2018-05-11

Tolerance to low-phosphorus soil is a desirable trait in soybean cultivars. Previous quantitative locus (QTL) studies for phosphorus-deficiency tolerance were mainly derived from bi-parental segregating populations and few reports natural population. The objective of this study was detect QTLs that regulate using association mapping approach. Phosphorus-deficiency evaluated according five traits (plant shoot height, dry weight, phosphorus concentration, acquisition efficiency use efficiency)...

10.1270/jsbbs.66.191 article EN Breeding Science 2016-01-01

Salinity threatens soybean germination, growth and production. The germination stage is a key period in the life of soybean. Wild contains many genes related to stress resistance that are valuable resources for genetic improvement To identify loci wild active during seed under salt stress, two populations, interspecific hybrid population comprising 142 lines natural 121 accessions, were screened three germination-related traits this study. By using single-nucleotide polymorphism (SNP)...

10.1016/j.jia.2022.07.031 article EN cc-by-nc-nd Journal of Integrative Agriculture 2022-01-01

Soybean provides essential protein and amino acids for humans animals, while sulfur-containing (SAA), including methionine (Met) cysteine (Cys), are very limited. In this study, we constructed a high-density bin-map with 3420 bin markers using 676 857 SNPs of recombinant-inbred line (RIL) population derived from cross between Kefeng no. 1 Nannong 1138-2. Quantitative trait loci (QTL) mapping was performed Cys, Met, SAA, the content bin-map. Twenty-five QTLs linked to these four traits were...

10.1021/acs.jafc.9b04497 article EN Journal of Agricultural and Food Chemistry 2019-10-16

Abstract Salinity is one of the major abiotic constraints affecting growth and yield plants including soybean. In this context, previous studies have documented role mitogen‐activated protein kinase (MAPK) cascade in regulation salt signaling model plants. However, there not a systematic analysis salt‐related MAPKs Hence, study, we identified total 32 GmMAPKs via., genome‐wide reanalysis MAPK family using soybean genome v4.0. Based on transcriptome datasets public database, observed that are...

10.1111/ppl.13549 article EN Physiologia Plantarum 2021-09-06

To understand the genetic basis of soybean sulfur utilization, a genome-wide association study (GWAS) and transcriptome analysis were used to discover new utilization genes in 121 wild soybeans. The shoot accumulation (SA) concentration (SC) seedlings growing Hoagland nutrient solution for 14 days evaluated greenhouse. maximum coefficients variation SA SC 66.79% 49.74%, respectively. An variance revealed that had significant differences among materials. Compared with SC, higher heritability...

10.3390/agronomy14020292 article EN cc-by Agronomy 2024-01-29

SUMMARY Soybean is an important plant source of protein worldwide. Increasing demands for soybean can be met by improving the quality its seed protein. In this study, GmCG‐1 , which encodes β‐conglycinin α′ subunit, was identified via combined genome‐wide association study and transcriptome analysis. We subsequently knocked down paralogues GmCG‐2 GmCG‐3 with CRISPR‐Cas9 technology generated two stable multigene knockdown mutants. As a result, content decreased, whereas 11S/7S ratio, total...

10.1111/tpj.17062 article EN The Plant Journal 2024-10-09
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